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Compound microbial agent for kitchen oil and wastewater treatment, and preparation method and applications of compound microbial agent

A compound microbial agent and wastewater treatment technology, which is applied in the field of microbial degradation, can solve the problems of oil pollution and other problems, and achieve the effects of stable performance, high oil degradation rate and reasonable formula

Inactive Publication Date: 2020-06-19
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to improve the treatment efficiency of grease degradation in view of the serious environmental pollution caused by grease pollutants

Method used

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  • Compound microbial agent for kitchen oil and wastewater treatment, and preparation method and applications of compound microbial agent
  • Compound microbial agent for kitchen oil and wastewater treatment, and preparation method and applications of compound microbial agent
  • Compound microbial agent for kitchen oil and wastewater treatment, and preparation method and applications of compound microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 adopts the composite bacterial agent of the present invention to degrade the grease wastewater of different concentrations

[0044] The specific operation steps are as follows:

[0045] 1. Preparation of oil-degrading compound bacterial agent:

[0046]Bacillus subtilis, Bacillus amyloliquefaciens, Citrobacter, Pseudomonas stutzeri, Pseudomonas fluorescens, Pseudomonas aeruginosa were added to the beef extract peptone medium, Penicillium was inoculated in the potato medium, For the expanded culture of the above-mentioned microorganisms, after the growth conditions of various strains are stable, according to Bacillus subtilis, Bacillus amyloliquefaciens, Citrobacter, Pseudomonas stutzeri, Pseudomonas fluorescens, Pseudomonas aeruginosa, The volume ratio of Penicillium polonium is 3: 2: 2: 1: 2: 2: 3 inoculated into QMM medium for seed expansion. After the growth of the bacteria is stable, the OD of the bacterial suspension is adjusted. 600 is 0.8±0.1, and t...

Embodiment 2

[0048] Embodiment 2 Oil-degrading bacterial agent is applied to the aerobic biological treatment of kitchen oil and fat wastewater

[0049] The specific operation steps are as follows:

[0050] 1. Bacillus subtilis, Bacillus amyloliquefaciens, Citrobacter, Pseudomonas stutzeri, Pseudomonas fluorescens, Pseudomonas aeruginosa, Penicillium polonium, Enterobacter cloacae, Serratia marcescens, Klebsiella mutans and Klebsiella pneumoniae were respectively inoculated into the corresponding medium for 2-4 days, and the microorganisms were expanded for culturing. 2: 4: 1: 2: 1: 2: 3: 2: 1: 2: 3 were inoculated into QMM medium for seed expansion. After the growth of the bacteria was stable, adjust the OD of the inoculum 600 is 0.8±0.1, that is, the oil-degrading compound bacterial agent is obtained, which is ready for use.

[0051] 2. The test kitchen grease wastewater was taken from the canteen of Southwest Jiaotong University and nearby kitchen enterprises. The grease wastewater wa...

Embodiment 3

[0052] Example 3 Anaerobic biological treatment of oil-degrading bacterial agent applied to kitchen waste oil and wastewater

[0053] The specific operation steps are as follows:

[0054] 1. Preparation of oil-degrading bacterial agent

[0055] Bacillus subtilis, Bacillus amyloliquefaciens, Citrobacter, Pseudomonas stutzeri, Pseudomonas fluorescens, Pseudomonas aeruginosa, Penicillium polonium, Enterobacter cloacae, Serratia marcescens, Klebsiella pneumoniae and Klebsiella pneumoniae were respectively inoculated into the corresponding medium for 2-4 days, and the microorganisms were expanded and cultured. After the growth of each strain was stable, the volume ratio was 6:4:3 : 1: 1: 2: 4: 2: 4: 5: 3 were inoculated into QMM medium for seed expansion, and after the growth of the bacteria was stable, adjust the OD of the inoculum 600 is 0.8±0.1, that is, the oil-degrading compound bacterial agent is obtained, which is ready for use.

[0056] 2. Treatment of kitchen waste oil ...

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Abstract

The invention belongs to the technical field of microbial degradation, and specifically relates to a compound microbial agent for kitchen oil and wastewater treatment, and a preparation method and applications of the compound microbial agent. In order to reduce the pollution of oil pollutants to environment, the compound microbial agent for kitchen oil and wastewater treatment is provided. The compound microbial agent includes Bacillus, Citrobacter and Pseudomonas, and the effective viable count ratio of each bacterium is 2-10: 1-4: 3-9. The preparation method of the compound microbial agent and applications of the compound microbial agent in oil degradation are also provided. Degradation can be performed by inoculating the compound microbial agent in oily wastewater or activated sludge during using according to the inoculating amount of 1.0-15%. The compound microbial agent is suitable for the treatment of kitchen wastewater, can reduce odor emission and can be industrially applied ona large scale; and the compound microbial agent is small in investment and high in efficiency and has no secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation, and in particular relates to a compound microbial inoculant for the treatment of kitchen grease wastewater and a preparation method and application thereof. [0002] technical background [0003] Grease wastewater is mainly from the kitchen industry and is an important component of domestic sewage. Because of its large output, it is easy to cause blockage of sewage treatment facilities and escape of foul odors. At present, the treatment methods of oily wastewater mainly include physical method, chemical method and biological method. Among them, the process paths involved in physical and chemical methods are generally complex, and are often prone to secondary pollution, and the investment cost is relatively high. The biological method has the advantages of stable treatment effect, low treatment cost, no secondary pollution, etc., and has broad application prospects. [0004] With th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/07C12R1/38C12R1/39C12R1/385C12R1/80C12R1/185C12R1/425C12R1/22C02F101/34
CPCC02F3/34C02F3/343C02F3/347C12N1/14C12N1/20
Inventor 赵锐邱忠平邹美慧徐柳彭传海毛继龙陶逸倩
Owner SOUTHWEST JIAOTONG UNIV
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