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A kind of spindle-shaped lysine bacillus strain and its application

A lysine bacillus, spindle-shaped technology, applied in the spindle-shaped lysine bacillus strain and its application field, can solve the problems of toxic substances flowing into the soil, occupying land resources, heavy metal pollution, etc., and achieve improved ability and improved Phosphorus absorption coefficient, stress-relieving effect

Active Publication Date: 2022-05-20
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All coal production areas basically store coal gangue in the open air, and the massive accumulation of coal gangue has caused many hazards: coal gangue hills occupy a large amount of land resources; weathering and spontaneous combustion of coal gangue will release toxic gases, generate dust, and cause heavy metal pollution. Pollution; the rain washes the gangue, causing the toxic substances in the gangue to flow into the soil and pollute the water quality
In short, the massive accumulation of coal gangue has caused waste of resources and serious environmental pollution, so it is of great significance to develop new methods of resource utilization of coal gangue

Method used

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  • A kind of spindle-shaped lysine bacillus strain and its application
  • A kind of spindle-shaped lysine bacillus strain and its application
  • A kind of spindle-shaped lysine bacillus strain and its application

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Experimental program
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Effect test

Embodiment

[0027] The separation of spindle-shaped lysine bacillus GZU-Lys01 comprises the following steps:

[0028] 1. Isolation and screening of functional strain GZU-Lys01

[0029] 1) Collection of samples

[0030] (1) The sample was collected from a coal gangue hill in a coal production area, and the fully weathered gangue sample and nearby soil samples were selected, and the collected samples were put into a collection bag;

[0031] (2) crush and sieve the sample collected, and collect a 100-mesh sample;

[0032] (3) Store the samples in a refrigerator at 4°C for later use.

[0033] 2) Screening of functional strains

[0034] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water;

[0035] (2) Shake in a constant temperature shaker at 180r / min, 27°C for 30min;

[0036] (3) After shaking for 30 minutes, take the supernatant and dilute to 1.0×10 -2 , 1.0×10 -3 , 1.0×10 -4 , 1.0×10 -5 , 1.0×10 -6 , 1.0×10 -7 .

[0037] (4) ...

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Abstract

The invention discloses a spindle-shaped lysine bacillus and an application thereof, which belongs to the genus Lysine bacillus and is named as a spindle-shaped lysine bacillus ( Lysinibacillus fusiformis ) GZU‑Lys01, which was deposited in China Center for Type Culture Collection on December 31, 2019, with the preservation number CCTCC NO: M20191140. Spindle-shaped lysine bacillus GZU-Lys01 of the present invention can effectively dissociate insoluble and / or insoluble phosphorus, potassium, nitrogen and other components in coal gangue, and transform them into available phosphorus, available potassium, hydrolyzed nitrogen and other plant absorption nutrient composition. GZU‑Lys01 strain makes it possible to reuse coal gangue as a resource.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a spindle-shaped lysine bacillus strain and its application. Background technique [0002] my country is a large country of coal production, and coal occupies an important position in my country's energy structure. The coal gangue produced in the process of coal mining and washing has become the largest industrial solid waste because it has not been effectively utilized. All coal production areas basically store coal gangue in the open air, and the massive accumulation of coal gangue has caused many hazards: coal gangue hills occupy a large amount of land resources; weathering and spontaneous combustion of coal gangue will release toxic gases, generate dust, and cause heavy metal pollution. Pollution: Rainwater scours coal gangue, causing toxic substances in coal gangue to flow into the soil and pollute water quality. In short, the massive accumulation of coal gangue ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/40C12R1/01
CPCC12N1/20C05G3/44C12R2001/01C12N1/205
Inventor 谢承卫高弦王应兰吉俐李小军董丽敏
Owner GUIZHOU UNIV
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