Microbial deodorant and preparation method thereof

A deodorant and microbial technology, applied in the field of microbial deodorant and its preparation, can solve the problems of inability to completely remove odorous substances, absorbents that cannot be reused, and secondary pollution of the environment, achieving high validity and low cost , the effect of improving the environment

Active Publication Date: 2014-08-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method absorbs the odorous substance into the micropores of the absorbent through the absorbent, but it is difficult to desorb the absorbent after absorbing the odorous substance, which makes the absorbent unable to be reused and causes secondary pollution to the environment
The chemical method reduces the odor by using chemical deodorants and fragrances. The use of chemical deodorants cannot completely remove the odorous substances. Fragrances will bring unpleasant odors due to the strong smell of spices.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] One of the present embodiment is used to remove NH 3 and H 2 The microbial deodorant of S malodorous gas, its preparation method comprises the following steps:

[0024] (1) Pseudomonas YF1, Sinorhizobium YS2 and Bacillus lysinica J2 were first activated, and then inoculated into the seed medium, and expanded at 28°C to 32°C until the strain concentration reached 1×10 8 Seed solutions of Pseudomonas YF1, Sinorhizobium YS2 and Bacillus lysinica J2 were obtained in the order of magnitude above individual / ml;

[0025] (2) The seed liquid of step (1) is inoculated respectively in the fermentation medium, and the inoculum amount is the seed liquid of every 100ml fermentation medium inoculation 2ml, and fermentation culture reaches 1 * 10 to strain concentration 8 The order of magnitude above individual / ml is obtained to obtain the fermentation broth of Pseudomonas YF1, Sinorhizobium YS2 and Bacillus lysinica J2;

[0026] Among them, the fermentation culture conditions of P...

Embodiment 2

[0031] In the present embodiment, the microbial deodorant is composed of Pseudomonas YF1 and Bacillus lysinica J2, and the preparation method comprises the following steps:

[0032] (1) Pseudomonas YF1 and Bacillus lysinica J2 were respectively activated first, then inoculated into the seed medium, and expanded at 28°C to 32°C until the strain concentration reached 1×10 8 The order of magnitude above individual / ml is obtained to obtain the seed liquid of Pseudomonas YF1 and Bacillus lysinica J2;

[0033] (2) The seed liquid of step (1) is inoculated respectively in the fermentation medium, and the inoculum amount is the seed liquid of every 100ml fermentation medium inoculation 2ml, and fermentation culture reaches 1 * 10 to strain concentration 8 Individual / ml above the order of magnitude, obtain the fermentation broth of Pseudomonas YF1 and Bacillus lysinica J2;

[0034] Among them, the fermentation and cultivation conditions of Pseudomonas YF1 are: temperature 28°C, pH 7.0, ...

Embodiment 3

[0038] In the present embodiment, the microbial deodorant is composed of Sinorhizobium YS2 and Bacillus lysinica J2, and the preparation method comprises the following steps:

[0039] (1) Activate Sinorhizobium YS2 and Bacillus lysinica J2 respectively, then inoculate them into the seed medium, and expand the cultivation at 28°C to 32°C until the strain concentration reaches 1×10 8 Individual / ml above the order of magnitude, to obtain the seed liquid of Sinorhizobium YS2 and Bacillus lysinica J2;

[0040] (2) The seed liquid of step (1) is inoculated respectively in the fermentation medium, and the inoculum amount is the seed liquid of every 100ml fermentation medium inoculation 2ml, and fermentation culture reaches 1 * 10 to strain concentration 8 The order of magnitude above individual / ml is obtained to obtain the fermentation broth of Sinorhizobium YS2 and Bacillus lysinica J2;

[0041] Among them, the conditions for the fermentation and cultivation of Sinorhizobium YS2 ar...

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PUM

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Abstract

The invention discloses a microbial deodorant and a preparation method thereof. The microbial deodorant is specially used for removing putrid odors generated by NH3 and H2S and comprises lysinibacillus fusiformis J2 which can absorb and utilize H2S and a bacterial strain with nitrosation ability. The bacterial strain with nitrosation ability is one of pseudomonas YF1 or sinorhizobium fredii YS2 or a mixed bacterial strain of YF1 and YS2. The bacterial strain concentrations of the pseudomonas YF1, sinorhizobium fredii YS2 and lysinibacillus fusiformis J2 are above order of magnitudes of 1*10<8>/ml. The deodorant disclosed by the invention has the advantages that the microbial deodorant has the utilizing effect to NH3 and H2S which generate putrid odors, and meanwhile further can obstruct emission of putrid odors and has a remarkable deodorizing effect. The preparation method of the microbial deodorant is simple, flow in process flow, low in cost and convenient to use, and the microbial deodorant can be stored at normal temperature with a long period of validity.

Description

technical field [0001] The invention relates to a deodorant, in particular to a microbial deodorant and a preparation method thereof. Background technique [0002] With the rapid development of large-scale and intensive farms in my country, the concentrated discharge of poultry manure has brought great pressure to the environment. Malodorous gas will be produced during the stacking of chicken manure, in which NH 3 and H 2 S is the main malodorous gas. Odor pollution to the environment is increasing, and it poses a great threat to poultry and human senses and health. Odor pollution has attracted more and more attention from all over the world. Therefore, the development and application of deodorants has become a research hotspot. [0003] Deodorants are mainly divided into physical deodorants, chemical deodorants, microbial deodorants, plant-based deodorants and compound deodorants. The physical method absorbs the odorous substance into the micropores of the absorbent thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L9/01C12N1/20C12R1/01A61L101/52
Inventor 李吕木甘晓徐雅芫
Owner ANHUI AGRICULTURAL UNIVERSITY
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