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Lysinibacillus fusiformis for rhizoctonia solani prevention and treatment

A technology of Bacillus lysinus and rice sheath blight bacteria, applied in the field of microbial control of rice sheath blight, can solve the problem of lack of new microbial pesticides for rice sheath blight

Inactive Publication Date: 2018-02-13
SHENYANG AGRI UNIV
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Problems solved by technology

[0004] In recent years, research on biological control of rice sheath blight using antagonistic microorganisms has been widely carried out in major rice regions in the world. The main products developed are Bacillus subtilis biocontrol agents. Most of the biocontrol strains of rice sheath blight are In the stage of laboratory research, there is still a lack of new microbial pesticides for the control of rice sheath blight in production

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  • Lysinibacillus fusiformis for rhizoctonia solani prevention and treatment
  • Lysinibacillus fusiformis for rhizoctonia solani prevention and treatment
  • Lysinibacillus fusiformis for rhizoctonia solani prevention and treatment

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Embodiment Construction

[0019] The present invention will be described in detail below with specific examples.

[0020] Screening, isolation and identification of strains

[0021] Isolation of strains: Isolation and purification were carried out by soil dilution separation method.

[0022] Screening of bacterial strains: using three-point plate confrontation culture method, mycelial growth rate method and sclerotia germination inhibition method, comprehensive screening of biocontrol bacteria that have biocontrol effects on rice sheath blight.

[0023] 1 plate confrontation culture method

[0024] Using the three-point plate confrontation culture method, select the rice sheath blight bacterial colony that has been activated and cultivated for 48 hours, and use a 5mm punch to make a fungus cake and place it in the center of the PDA plate. The 3 points were displaced for testing bio-control bacteria. Incubate at a constant temperature of 27°C for 72h. Observe the formation of the zone of inhibition ...

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Abstract

The invention discloses lysinibacillus fusiformis for rhizoctonia solani inhibition. A microbial strain and agricultural chemical products thereof are provided and are nontoxic to human and animals, environmentally friendly and effective in rhizoctonia solani prevention. Microbial bactericides for rhizoctonia solani prevention and treatment are diversified.

Description

technical field [0001] The invention relates to the technical field of microbial control of rice sheath blight, in particular to a spindle-shaped lysine bacillus for preventing and treating rice sheath blight. Background technique [0002] Rice sheath blight (Rhizoctonia solani) is one of the most serious and difficult fungal diseases at home and abroad. It is widely distributed and seriously damaged, causing huge economic losses to agricultural production every year. Due to the strong saprophytic ability and wide range of hosts of sheath blight, no high resistance varieties to the disease have been found at home and abroad. At present, Jinggangmycin and some chemical pesticides are mainly used to prevent and control rice sheath blight in my country. The long-term repeated use of antibiotics and chemical agents has caused the pathogenic bacteria to produce obvious drug resistance, resulting in a decrease or even loss of control effect. The long-term large-scale use of pestic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A61P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 魏松红罗文芳王海宁李思博李帅
Owner SHENYANG AGRI UNIV
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