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Lysinibacillus fusiformis preparation for degrading uric acid as well as preparation method and application of lysinibacillus fusiformis preparation

A technology of Bacillus lysinus and Bacillus, applied in the biological field, can solve problems such as side effects, uric acid drugs or food effects are not significant

Active Publication Date: 2021-12-14
UNIV OF SCI & TECH BEIJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to solve the problem that the medicine or food that degrades and removes uric acid in the prior art has no significant effect and side effects, and provides a method for Bacillus that biodegrades uric acid. The bacterial cells, spores and the enzymes produced by this strain are safe for the human body, and can efficiently biodegrade uric acid. It has important application prospects in the biodegradation and removal of uric acid.

Method used

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  • Lysinibacillus fusiformis preparation for degrading uric acid as well as preparation method and application of lysinibacillus fusiformis preparation
  • Lysinibacillus fusiformis preparation for degrading uric acid as well as preparation method and application of lysinibacillus fusiformis preparation
  • Lysinibacillus fusiformis preparation for degrading uric acid as well as preparation method and application of lysinibacillus fusiformis preparation

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Experimental program
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preparation example Construction

[0072] The third aspect of the present invention provides a method for preparing the uric acid-degrading spindle-shaped lysine bacillus preparation as described in the second aspect of the present invention, which includes:

[0073] Step B, inoculate the fermentation strain into the fermentation medium, and carry out fermentation and culture for 3-5 days at 18-40°C, preferably 36-38°C, at a shaker speed of 100-300 rpm, to obtain Bacillus fermentation culture;

[0074] Step C, performing centrifugation on the fermentation culture of Bacillus, and harvesting the bacteria cells and / or spores of Bacillus;

[0075] Wherein, the fermented strains are obtained from corresponding bacterial strains through seed cultivation.

[0076] As known to those skilled in the art, at present, 16S rRNA is often used internationally for molecular identification of bacteria, so 16S rRNA can be used for comparison of similarities to obtain their homology. Therefore, the fermentation strains used in...

Embodiment approach

[0092] According to some embodiments of the present invention, the preparation method also includes:

[0093] Step K, performing cell disruption treatment on the cell suspension of Bacillus in an ice-water bath (i.e. ice-water mixture, 0-4° C.) to obtain a cell-free disruption solution of Bacillus;

[0094] Step L, centrifuging the cell-free crushed solution of Bacillus, and taking the supernatant cell-free extract as the crude enzyme of Bacillus spindle-shaped Lysinus strain YC-01.

[0095] The present invention has no particular limitation on the conditions of the centrifugation in the above step L. In some embodiments of the present invention, for example, the centrifugation of the to-be-separated product can be performed at 15000-18000 r / min for 10-20 min.

[0096] The fourth aspect of the present invention provides a spindle-shaped lysine Bacillus preparation for biodegrading uric acid described in the second aspect of the present invention or the spindle-shaped lysine fo...

Embodiment 1

[0113] (1) Prepare the growth medium of Bacillus spindle-shaped lysinus YC-01, which consists of (per liter): 10.0 g of beef extract, 10.0 g of yeast powder, and 5.0 g of NaCl. Add 100 milliliters of the prepared liquid medium into a 500 milliliter conical flask, sterilize at high temperature and pressure (121° C.) for 20 minutes, and then sterilize for another 20 minutes under ultraviolet irradiation in a clean bench.

[0114] (2) Under sterile conditions in a clean bench, inoculate 0.5 ml of Bacillus fusiformis lysinus YC-01 bacteria solution into the liquid culture medium of the Erlenmeyer flask at a temperature of 38°C and a shaker speed of 200 rpm. After 3 days of batch culture, the cells and / or spores of Bacillus lysinica spindle-shaped YC-01 were harvested by centrifuging (8000 rpm, 10 minutes) and discarding the supernatant.

[0115] Take 20mL of the cell suspension of Bacillus spindle-shaped lysinica YC-01 and add it to a 50mL glass tube, insert it into ice water, and...

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Abstract

The invention belongs to the technical field of biology, and relates to bacillus for biodegradation of uric acid, the bacillus can generate enzyme for catalytic degradation of uric acid, and the bacillus is lysinibacillus fusiformis YC-01 strain. The invention also relates to a lysinibacillus fusiformis preparation for degrading uric acid. The lysinibacillus fusiformis preparation contains one or more of bacterial cells, spores and crude enzyme of the bacillus. Research results show that the bacillus for biodegrading uric acid and the enzyme produced by the bacillus are safe to human bodies, can efficiently biodegrade uric acid, and have important application prospects in the aspects of removing uric acid and treating human hyperuricemia and corresponding complications.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a spindle-shaped lysine bacillus preparation for degrading uric acid, a preparation method and application thereof. Background technique [0002] In recent years, the incidence of hyperuricemia (HUA) and its resulting complications such as gout, obesity, atherosclerosis, hypertension, and diabetes has gradually increased, and the age of patients has gradually become younger It has become the second largest chronic metabolic disease after diabetes. HUA refers to the phenomenon that the uric acid content in the blood obviously exceeds the normal value, which is an important hidden danger leading to human health problems. In the human body, purines and other substances undergo a series of physiological and biochemical reactions to generate uric acid, which is the main synthetic route. Human uric acid is mainly excreted through the kidneys, and the rest is excreted through the intestinal...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P19/06C12R1/01
CPCC12N1/20A61K35/742A61P19/06Y02A50/30
Inventor 闫海庞煜滕俊辉赵真真刘洋许倩倩吴安情徐止开
Owner UNIV OF SCI & TECH BEIJING
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