Composite microbial inoculum of lysinibacillus and bacillus subtilis and preparation method thereof

A technology of Bacillus lysinus and Bacillus subtilis, applied in the field of agricultural microorganisms, can solve the problems of destroying the quality and safety of agricultural products, the ecological environment of pesticide residues, soil heavy metal pollution, etc., achieve significant antibacterial and disease prevention effects, promote good growth, and help The effect of sustainable development

CN109504622AActive Publication Date: 2019-03-22GUANGDONG GEOLONG BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-03-22

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Abstract

The invention discloses a composite microbial inoculum of lysinibacillus and bacillus subtilis and a preparation method thereof, and belongs to the technical field of agricultural microbes. The composite microbial inoculum is prepared by blending and confecting bacillus subtilis R31 fermentation broth and lysinibacillus fusiformis LW-3 fermentation broth, wherein the collection number of the lysinibacillus fusiformis LW-3 is CGMCC (China General Microbiological Culture Collection Center) No. 16228; and the mixing proportion of the bacillus subtilis R31 fermentation broth to the lysinibacillusfusiformis LW-3 fermentation broth is (1 to 10) to (10 to 1). The composite microbial inoculum provided by the invention has obvious growth-promotion, antibacterial and disease-prevention effects on aplant, can obviously inhibit the occurrences of diseases of bacterial wilt, blight and root rot and the like, improves a yield and quality, has the characteristics of being safe, efficient, nontoxicand pollution-free, can effectively reduce the use of a chemical fertilizer and a pesticide, relieves environmental pollution pressure, and assists agricultural sustainable development.
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Description

technical field

[0001] The invention relates to a bacillus composite bacterial agent and a preparation method thereof, in particular to a composite bacterial agent of lysine bacillus and bacillus subtilis and a preparation method thereof, belonging to the technical field of agricultural microorganisms. Background technique

[0002] Phosphorus is an important nutrient element in the process of plant growth. Phosphorus is a component of nucleic acid and nucleotides, and is an important element that forms the nucleus and various organelles. Phosphorus deficiency will affect the division and formation of plant cells, resulting in poor root development and slow or even stagnant plant growth. In addition, phosphorus is a component of phospholipids, adenophosphorus and many enzymes, and is an important participatory substance in various processes of plant photosynthesis. When phosphorus is deficient, the leaves are dark green and dull, the plants are short, and the resistance is we...

Examples

Embodiment 1

[0053] 1. Isolation of spindle-shaped lysinibacillus LW-3

[0054] Naturally air-dry the soil collected from the ancient well in Jiangmen, Guangdong, and grind it into powder to obtain a soil sample; weigh 5g of the sample, dissolve it in a triangular flask filled with 45ml of sterile water and glass beads, and vibrate with a 150rp oscillator; after shaking, it becomes 10-1 times Place the Erlenmeyer flask in a water bath at 80°C for 30 minutes, then use a pipette to pipette 1ml into a test tube filled with 9ml of sterile water, oscillate fully to obtain a 10-2-fold dilution, and then dilute to 10-5 times dilution;

[0055] Prepare a phosphorus-dissolving and potassium-dissolving medium, sterilize at 121°C for 30 minutes, take 0.1ml of the diluted solution, spread it on the medium, and then place it in an incubator for cultivation, observe the strains that produce phosphorus-dissolving circles and potassium-dissolving circles, and pick Get and streak on the nutrient agar (NA)...