Composite microbial inoculum of lysinibacillus and bacillus subtilis and preparation method thereof
A technology of Bacillus lysinus and Bacillus subtilis, applied in the field of agricultural microorganisms, can solve the problems of destroying the quality and safety of agricultural products, the ecological environment of pesticide residues, soil heavy metal pollution, etc., achieve significant antibacterial and disease prevention effects, promote good growth, and help The effect of sustainable development
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[0053] 1. Isolation of spindle-shaped lysinibacillus LW-3
[0054] Naturally air-dry the soil collected from the ancient well in Jiangmen, Guangdong, and grind it into powder to obtain a soil sample; weigh 5g of the sample, dissolve it in a triangular flask filled with 45ml of sterile water and glass beads, and vibrate with a 150rp oscillator; after shaking, it becomes 10-1 times Place the Erlenmeyer flask in a water bath at 80°C for 30 minutes, then use a pipette to pipette 1ml into a test tube filled with 9ml of sterile water, oscillate fully to obtain a 10-2-fold dilution, and then dilute to 10-5 times dilution;
[0055] Prepare a phosphorus-dissolving and potassium-dissolving medium, sterilize at 121°C for 30 minutes, take 0.1ml of the diluted solution, spread it on the medium, and then place it in an incubator for cultivation, observe the strains that produce phosphorus-dissolving circles and potassium-dissolving circles, and pick Get and streak on the nutrient agar (NA)...
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