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Composite microbial inoculum of lysinibacillus and bacillus subtilis and preparation method thereof

A technology of Bacillus lysinus and Bacillus subtilis, applied in the field of agricultural microorganisms, can solve the problems of destroying the quality and safety of agricultural products, the ecological environment of pesticide residues, soil heavy metal pollution, etc., achieve significant antibacterial and disease prevention effects, promote good growth, and help The effect of sustainable development

Active Publication Date: 2019-03-22
GUANGDONG GEOLONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, due to the excessive application of chemical fertilizers and pesticides, a series of ecological environment damage problems and agricultural product quality and safety problems have followed one after another, such as soil compaction, soil heavy metal pollution, water pollution, air pollution, and pesticide residues. It has become a major obstacle to the sustainable development of agriculture. Therefore, it is urgent to find green, safe and efficient methods to increase crop production and income and help sustainable agricultural development.
[0006] Microbial fertilizers have the advantages of being green, safe, efficient, non-toxic, and non-polluting, and meet the requirements of "environmental protection" and "green". urgent
[0007] Therefore, in order to overcome the defect of a single action of a single bacterial agent, improve the efficacy of fertilizers and pesticides, effectively reduce the use of chemical fertilizers and pesticides, and meet the needs of agricultural production while avoiding environmental pollution, provide an alternative to chemical fertilizers and pesticides that can promote plant growth. The development of compound bacterial agents capable of antibacterial and disease prevention has become an urgent technical problem in this technical field.

Method used

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  • Composite microbial inoculum of lysinibacillus and bacillus subtilis and preparation method thereof
  • Composite microbial inoculum of lysinibacillus and bacillus subtilis and preparation method thereof
  • Composite microbial inoculum of lysinibacillus and bacillus subtilis and preparation method thereof

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Effect test

Embodiment 1

[0053] 1. Isolation of spindle-shaped lysinibacillus LW-3

[0054] Naturally air-dry the soil collected from the ancient well in Jiangmen, Guangdong, and grind it into powder to obtain a soil sample; weigh 5g of the sample, dissolve it in a triangular flask filled with 45ml of sterile water and glass beads, and vibrate with a 150rp oscillator; after shaking, it becomes 10-1 times Place the Erlenmeyer flask in a water bath at 80°C for 30 minutes, then use a pipette to pipette 1ml into a test tube filled with 9ml of sterile water, oscillate fully to obtain a 10-2-fold dilution, and then dilute to 10-5 times dilution;

[0055] Prepare a phosphorus-dissolving and potassium-dissolving medium, sterilize at 121°C for 30 minutes, take 0.1ml of the diluted solution, spread it on the medium, and then place it in an incubator for cultivation, observe the strains that produce phosphorus-dissolving circles and potassium-dissolving circles, and pick Get and streak on the nutrient agar (NA)...

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Abstract

The invention discloses a composite microbial inoculum of lysinibacillus and bacillus subtilis and a preparation method thereof, and belongs to the technical field of agricultural microbes. The composite microbial inoculum is prepared by blending and confecting bacillus subtilis R31 fermentation broth and lysinibacillus fusiformis LW-3 fermentation broth, wherein the collection number of the lysinibacillus fusiformis LW-3 is CGMCC (China General Microbiological Culture Collection Center) No. 16228; and the mixing proportion of the bacillus subtilis R31 fermentation broth to the lysinibacillusfusiformis LW-3 fermentation broth is (1 to 10) to (10 to 1). The composite microbial inoculum provided by the invention has obvious growth-promotion, antibacterial and disease-prevention effects on aplant, can obviously inhibit the occurrences of diseases of bacterial wilt, blight and root rot and the like, improves a yield and quality, has the characteristics of being safe, efficient, nontoxicand pollution-free, can effectively reduce the use of a chemical fertilizer and a pesticide, relieves environmental pollution pressure, and assists agricultural sustainable development.

Description

technical field [0001] The invention relates to a bacillus composite bacterial agent and a preparation method thereof, in particular to a composite bacterial agent of lysine bacillus and bacillus subtilis and a preparation method thereof, belonging to the technical field of agricultural microorganisms. Background technique [0002] Phosphorus is an important nutrient element in the process of plant growth. Phosphorus is a component of nucleic acid and nucleotides, and is an important element that forms the nucleus and various organelles. Phosphorus deficiency will affect the division and formation of plant cells, resulting in poor root development and slow or even stagnant plant growth. In addition, phosphorus is a component of phospholipids, adenophosphorus and many enzymes, and is an important participatory substance in various processes of plant photosynthesis. When phosphorus is deficient, the leaves are dark green and dull, the plants are short, and the resistance is we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07C12R1/125
CPCC12N1/20A01N63/10
Inventor 王勇温书恒陈燕琼武展梁仕权
Owner GUANGDONG GEOLONG BIOTECH
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