Spindle-shaped lysinibacillus and its application
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[0026] 1. Isolation of spindle-shaped lysinibacillus LW-3
[0027] Naturally air-dry the soil collected from the ancient well in Jiangmen, Guangdong, and grind it into powder to obtain a soil sample; weigh 5g of the sample, dissolve it in a triangular flask filled with 45ml of sterile water and glass beads, and vibrate with a 150rp oscillator; after shaking, it becomes 10-1 times Place the Erlenmeyer flask in a water bath at 80°C for 30 minutes, then use a pipette to pipette 1ml into a test tube filled with 9ml of sterile water, oscillate fully to obtain a 10-2-fold dilution, and then dilute to 10-5 times dilution;
[0028] Prepare a phosphorus-dissolving and potassium-dissolving medium, sterilize at 121°C for 30 minutes, take 0.1ml of the diluted solution, spread it on the medium, and then place it in an incubator for cultivation, observe the strains that produce phosphorus-dissolving circles and potassium-dissolving circles, and pick Get and streak on the nutrient agar (NA)...
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