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Application of siderophore plant-growth-promoting rhizobacteria

A rhizosphere growth-promoting bacteria and siderophore technology, applied in the field of microorganisms, can solve the problems of high cost, soil acidification or salinization, and long time required, and achieve the goals of increasing plant height, long-lasting effect, and preventing acidification Effect

Pending Publication Date: 2022-01-07
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Partial soil acidification is to reduce the pH value of the soil by applying acidifying agents to the soil, thereby increasing the available iron content in the soil to improve camphor yellows. However, it takes a long time to improve the use of acidifying agents, and the traditional irrigation method Applying acidifiers can be costly and long-term use can cause soil acidification or salinization

Method used

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  • Application of siderophore plant-growth-promoting rhizobacteria
  • Application of siderophore plant-growth-promoting rhizobacteria
  • Application of siderophore plant-growth-promoting rhizobacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one: Fermentation of siderophore rhizosphere growth-promoting bacteria

[0027] The fermentation method of the siderophore rhizosphere growth-promoting bacteria is as follows: transfer the spindle-shaped lysine bacillus to the LB liquid medium with a pH of 8.0-10.0, and shake it at 26-30°C and 140-160r / min until Obtain the seed bacterial liquid in the logarithmic growth phase; inoculate the seed bacterial liquid in the fresh LB liquid medium with a pH of 8.5-10.5 according to the inoculation amount of 2% by volume, and shake it under the conditions of 26-30°C and 140-160r / min Cultivate and ferment for 40-48 hours to obtain a fermented liquid.

[0028] Calculate the number of effective viable bacteria in the fermentation broth by means of plate counting, then centrifuge the fermentation broth, discard the supernatant, and dilute the fermentation broth with water so that the concentration of viable bacteria is 10 9 CFU / mL fermentation diluent for later use.

Embodiment 2

[0029] Embodiment two: Potted camphor is inoculated with spindle-shaped lysine bacillus

[0030] Camphor seedlings: Camphor seeds come from Xuzhou Seed Co., Ltd., select full-grained camphor seeds, soak them in hot water at 70-80°C, the volume ratio of water to seeds is 2:1, stir well until the water temperature is 50-60 between 28°C and 28°C, cool naturally, take it out after 24 hours, wrap it in a wet towel after cleaning, and place it in the incubator to accelerate germination. Wash it once a day to remove the sticky matter on the surface of the seeds. , after the seeds emerge, transplant them into pots.

[0031] Select 10 pots of camphor seedlings, of which 5 pots are used to inoculate spindle-shaped lysine bacillus, and water once after 2 months of emergence with a live bacterial concentration of 10 9CFU / mL fermentation diluent 50ml, the other 5 pots were not inoculated with spindle-shaped lysine bacillus (CK control group), watered 50ml of tap water once as a control at...

Embodiment 3

[0044] Embodiment three: camphor tree inoculated with spindle-shaped lysine bacillus

[0045] The live bacteria concentration was 10 9 CFU / mL fermented diluent is poured or sprayed in the garden green land planted with camphor trees; the soil properties of the green land are shown in Table 4, and each camphor tree is inoculated with 2L or 4L, inoculated in spring, and the two inoculation treatments are separated by half a month Carry out the second inoculation, measure chlorophyll and the iron element content in the leaf after the second inoculation 1 month, count the yellowing situation of camphor, first observe the whole plant situation of the plant, then the upper, middle and lower three layers of the camphor tree crown, One branch was taken from each layer in the southeast and northwest for specific statistics. The overall and specific statistics were considered comprehensively, and the yellowing level of the plants was classified. The results are shown in Table 4.

[004...

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Abstract

The invention relates to application of siderophore plant-growth-promoting rhizobacteria, in particular to application of lysinibacillus fusiformis to prevention and / or treatment of iron chlorosis of cinnamomum camphora. The fermentation method of the siderophore plant-growth-promoting bacteria comprises the following steps of: transferring lysinibacillus fusiformis into an LB liquid culture medium with the pH value of 8.0-10.0, and carrying out shake culture under the conditions of 26-30 DEG C and 140-160r / min until the logarithmic phase to obtain a seed bacteria liquid; inoculating the seed bacteria liquid into a fresh LB liquid culture medium with the pH value of 8.5-10.5 according to the inoculum size of 2% by volume, and performing shaking culture and fermentation to obtain fermentation liquid; and calculating the effective viable count in the fermentation liquid, then centrifuging the fermentation liquid, discarding supernate, diluting the fermentation liquid with water to obtain a fermentation diluent with the viable count of 10<9>CFU / mL, and then irrigating or spraying the fermentation diluent into soil for planting cinnamomum camphora. The siderophore plant-growth-promoting rhizobacteria can be used for effectively preventing and treating iron chlorosis of cinnamomum camphora, and is simple in process, environment-friendly, long in lasting period and low in cost.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of siderophore rhizosphere growth-promoting bacteria. Background technique [0002] Cinnamomum camphora Linn. is an evergreen tree of the genus Cinnamomum camphora in the family Lauraceae. It is a representative tree species of subtropical evergreen broad-leaved forest and one of the five famous tree species in the world. The camphor tree is neat and beautiful in shape, has high ornamental value, and has functions such as wind resistance, smoke resistance, and absorption of various toxic gases. It is an important economic tree species and landscaping tree species in my country. It is mostly cultivated in Jiangsu and the southern provinces of our country. Cinnamomum camphora is an iron-deficiency sensitive plant, and its iron-deficiency chlorosis has been widely reported in the cultivated areas. [0003] Iron deficiency chlorosis is a physiological disease...

Claims

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Application Information

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IPC IPC(8): A01N63/22A01P21/00C05G3/00C05G3/40C12N1/20C12R1/01
CPCA01N63/22C05G3/00C05G3/40C12N1/20
Inventor 琚淑明李培根朱芝宜李林玉宋珂杨震
Owner XUZHOU UNIV OF TECH
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