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Lysinibacillus fusiformis with methylamine degradation capability and application thereof

A Clostridium and methylamine technology, applied in the field of microorganisms, can solve problems such as limited degradation ability, and achieve the effect of efficiently degrading methylamine

Active Publication Date: 2018-03-20
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, due to the limited ability of a single strain to degrade methylamine, some researchers have studied the degradation of methylamine by inoculating mixed strains, but there are few studies on the degradation of methylamine using a new single strain. literature report

Method used

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  • Lysinibacillus fusiformis with methylamine degradation capability and application thereof
  • Lysinibacillus fusiformis with methylamine degradation capability and application thereof
  • Lysinibacillus fusiformis with methylamine degradation capability and application thereof

Examples

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Effect test

Embodiment 1

[0026] A Clostridium strain with the ability to degrade methylamine, the name of the strain is Clostridium (Lysinibacillus fusiformis) GDUTAN2, preserved in the China Center for Type Culture Collection, and the preservation address is 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province No. Wuhan University; the deposit number is CCTCC NO.: M 2017284, and the deposit date is May 24, 2017.

[0027] Clostridium GDUTAN2 in this example was isolated and screened from the leachate of a landfill in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: used acclimatization medium is inorganic salt medium g / L (every 1000mL inorganic salt medium contains K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, 1mL trace element solution (g / L): CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O0.2g, CoCl 2 ·6H 2 O 0.09g, Na 2 MoO 4 2H 2 O 0.12g, H 3 BO 3 0....

Embodiment 2

[0046] This example is the application of Clostridium (Lysinibacillus fusiformis) GDUTAN2 in environmental remediation, which can degrade methylamine in the environment. The environment includes the atmosphere, water body or soil.

[0047] The method that the methylamine degradability of Clostridium (Lysinibacillus fusiformis) GDUTAN2 obtained by the screening of the present invention is tested and verified below is as follows:

[0048] According to the needs of the degradation experiment, configure the inorganic salt medium: add 100 mL of inorganic salt solution to the 300 mL serum bottle (each 100 mL of inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.12g, KH 2 PO 4 0.12g, NH 4 Cl 0.04g, MgSO 4 ·7H 2 O 0.02g, FeSO 4 ·7H 2 O 0.001g, CaCl 2 2H 2 O 0.02g, MnSO 4 4H 2 O 0.02g, CuSO 4 2H 2 O0.001g, ZnSO 4 ·7H 2 O 0.02g, CoCl 2 ·6H 2 O 0.009g, Na 2 MoO 4 2H 2 O 0.012g, H 3 BO 3 0.0006g, double distilled water 100mL), autoclaved at 121℃ for 30min. Fir...

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Abstract

The invention provides lysinibacillus fusiformis with methylamine degradation capability and application thereof. The name of the strain is lysinibacillus fusiformis GDUTAN2, and is preserved in CCTCC(China Center for Type Culture Collection); the preservation address is Wuhan University, Wuhan, Hubei province; the preservation code is CCTCC NO:M 2017284; the preservation date is May, 24, 2017. The lysinibacillus fusiformis GDUTAN2 is gram-positive, and is in rhabditiform; the colonial morphology is in a round shape, white color and transparent state; the colonial diameter is 1 to 2mm. The lysinibacillus fusiformis GDUTAN2 provided by the invention can be applied to environment restoration for degrading the methylamine in the environment; the degradation rate is high; the degradation ratecan reach 96.3 percent after the degradation for 96h under the condition of the substrate concentration being 5mg / L.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a Clostridium bacterium with methylamine degrading ability and application thereof. Background technique [0002] Odor pollution is a major problem of air pollution today. Organic malodorous gases are mainly divided into sulfur-containing organic malodorous gases and nitrogen-containing organic malodorous gases, among which methylamine (MA) is a typical nitrogen-containing organic malodorous compound. The olfactory threshold of methylamine is as low as 0.021ppm, making it difficult to biodegrade. When the concentration of methylamine is low, a strong irritating fish odor can be produced, and it is easily absorbed by the skin and clothes. Methylamine can be absorbed through the respiratory tract, gastrointestinal tract and skin, and converted into dimethylamine or oxidized into formic acid in the body. Methylamine has a strong stimulating and corrosive ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/32B01D53/84B09C1/10C12R1/01
CPCB01D53/84C02F3/32B09C1/10B01D2257/70B01D2257/306C12R2001/01C12N1/205Y02A50/20B09C1/00B09C1/002B09C1/105B09C2101/00C02F3/341B01D2251/95B01D2257/40B01D2258/06C12N1/20
Inventor 李桂英李世蔼安太成苏岳檀宁寻安
Owner GUANGDONG UNIV OF TECH
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