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A strain of clostridia with methylamine degrading ability and its application
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一种梭状芽孢杆菌、甲胺的技术，应用在微生物领域，能够解决降解能力有限等问题，达到高效降解甲胺的效果
Active Publication Date: 2019-08-23
GUANGDONG UNIV OF TECH
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According to reports, due to the limited ability of a single strain to degrade methylamine, some researchers have studied the degradation of methylamine by inoculating mixed strains, but there are few studies on the degradation of methylamine using a new single strain. literature report
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[0026] A Clostridium strain with the ability to degrade methylamine, the name of the strain is Clostridium (Lysinibacillus fusiformis) GDUTAN2, preserved in the China Center for Type Culture Collection, and the preservation address is 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province No. Wuhan University; the deposit number is CCTCC NO.: M 2017284, and the deposit date is May 24, 2017.
[0027] Clostridium GDUTAN2 in this example was isolated and screened from the leachate of a landfill in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: used acclimatization medium is inorganic salt medium g / L (every 1000mL inorganic salt medium contains K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, 1mL trace element solution (g / L): CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O0.2g, CoCl 2 ·6H 2 O 0.09g, Na 2 MoO 4 2H 2 O 0.12g, H 3 BO 3 0....
[0047] The method that the methylamine degradability of Clostridium (Lysinibacillus fusiformis) GDUTAN2 obtained by the screening of the present invention is tested and verified below is as follows:
[0048] According to the needs of the degradation experiment, configure the inorganic salt medium: add 100 mL of inorganic salt solution to the 300 mL serum bottle (each 100 mL of inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.12g, KH 2 PO 4 0.12g, NH 4 Cl 0.04g, MgSO 4 ·7H 2 O 0.02g, FeSO 4 ·7H 2 O 0.001g, CaCl 2 2H 2 O 0.02g, MnSO 4 4H 2 O 0.02g, CuSO 4 2H 2 O0.001g, ZnSO 4 ·7H 2 O 0.02g, CoCl 2 ·6H 2 O 0.009g, Na 2 MoO 4 2H 2 O 0.012g, H 3 BO 3 0.0006g, double distilled water 100mL), autoclaved at 121℃ for 30min. Fir...
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Abstract
The invention provides a Clostridium bacterium with methylamine degrading ability and application thereof. The name of the strain is Clostridium fusiformis (Lysinibacillus fusiformis) GDUTAN2, and it is preserved in the China Center for Type Culture Collection at the campus of Wuhan University in Wuhan, Hubei Province; the preservation number is CCTCC NO: M 2017284, and the preservation date is May 2017 24th. The clostridium bacterium GDUTAN2 is Gram-positive, rod-shaped, and the colony is round, white, and transparent, and the diameter of the colony is 1-2 mm. The clostridium bacterium GDUTAN2 of the present invention can be applied to environmental restoration, degrades methylamine in the environment, and has a high degradation rate, and the degradation rate can reach 96.3% when the substrate concentration is 5 mg / L for 96 hours.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a Clostridium bacterium with methylamine degrading ability and application thereof. Background technique [0002] Odorpollution is a major problem of air pollution today. Organic malodorous gases are mainly divided into sulfur-containing organic malodorous gases and nitrogen-containing organic malodorous gases, among which methylamine (MA) is a typical nitrogen-containing organic malodorous compound. The olfactory threshold of methylamine is as low as 0.021ppm, making it difficult to biodegrade. When the concentration of methylamine is low, a strong irritating fish odor can be produced, and it is easily absorbed by the skin and clothes. Methylamine can be absorbed through the respiratory tract, gastrointestinal tract and skin, and converted into dimethylamine or oxidized into formic acid in the body. Methylamine has a strong stimulating and corrosive ef...
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