Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Bacillus cereus having the ability to degrade monomethylamine and its application

A technology of Bacillus cereus and methylamine, applied in the field of microorganisms, can solve the problem of not seeing Bacillus cereus degrading monomethylamine and the like

Active Publication Date: 2021-02-09
紫科装备股份有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no relevant reports and patents on the use of Bacillus cereus to degrade monomethylamine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Bacillus cereus having the ability to degrade monomethylamine and its application
  • A kind of Bacillus cereus having the ability to degrade monomethylamine and its application
  • A kind of Bacillus cereus having the ability to degrade monomethylamine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A strain of Bacillus cereus with the ability to degrade methylamine. The name of the strain is Bacillus cereus (Bacillus cereus) GDUTAN3. It is preserved in the China Center for Type Culture Collection at 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province No. Wuhan University campus; the deposit number is CCTCC NO: M 2018229, and the deposit date is April 23, 2018.

[0026] The Bacillus cereus GDUTAN3 of this example was isolated and screened from the leachate of a landfill in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: the acclimatization medium used is inorganic salt medium g / L (every 1000 mL inorganic salt medium contains K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, 1mL trace element solution (g / L): CaCl 2 2H 2 O 0.2 g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09 g, Na 2 MoO 4 2H 2 O 0.12g, H 3 BO 3 ...

Embodiment 2

[0047] This example is the application of Bacillus cereus GDUTAN3 in environmental remediation, which can degrade methylamine in the environment. The environment includes the atmosphere, water body or soil.

[0048] Below the method that the methylamine degradation ability of the Bacillus cereus (Bacillus cereus) GDUTAN3 that the present invention screens obtains is tested and verified is as follows:

[0049] According to the needs of the degradation experiment, configure inorganic salt medium, add 100 mL of inorganic salt solution to the 300 mL serum bottle (every 100 mL of inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.12g, KH 2 PO 4 0.12g, NH 4 Cl 0.04g, MgSO 4 ·7H 2 O 0.02g, FeSO 4 ·7H 2 O 0.001g, CaCl 2 2H 2 O 0.02g, MnSO 4 4H 2 O 0.02g, CuSO 4 2H 2 O0.001g, ZnSO 4 ·7H 2 O 0.02g, CoCl 2 ·6H 2 O 0.009g, Na 2 MoO 4 2H 2 O 0.012g, H 3 BO 3 0.0006g, double distilled water 100mL), autoclaved at 121℃ for 30min. First, put the screened Bacillus ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a Bacillus cereus capable of degrading monomethylamine and its application in environmental restoration. By separating and purifying the leachate of the waste landfill in Guangzhou, the present invention screened out a Bacillus cereus strain with good degrading ability to the nitrogen-containing malodorous pollutant monomethylamine, and named the Bacillus cereus as wax Bacillus cereus GDUTAN3 is deposited in the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan City, Hubei Province; the deposit number is CCTCC NO.: M 2018229. The deposit date is April 23, 2018. The Bacillus cereus GDUTAN3 is gram-positive, rod-shaped, and the colony shape is round, white, and opaque, and the colony diameter is 2-3 mm. The Bacillus cereus GDUTAN3 obtained by the invention has better degradation effect on the odor pollutant methylamine in the environment, and can be applied to environmental restoration. Under the condition of methylamine concentration of 5~130mg / L, it is degraded for 96h, and the degradation rate can reach more than 94.8%.

Description

technical field [0001] The invention relates to a microbial technology, in particular to a bacillus cereus capable of degrading monomethylamine and its application. Background technique [0002] Organic amine compounds are odorous pollutants second only to organic sulfides. They are raw materials or intermediates in chemical industries such as pesticides, dyes, and pharmaceuticals. They are often detected in wastewater and waste gas discharged by related chemical companies, and most of them have High toxicity, migration and bioaccumulation have great impact on human health. Therefore, the behavior of organic amines in the environment has attracted extensive attention in recent years. [0003] And one methylamine (Methylamine, CH 3 NH 2 ) is an important aliphatic amine, which is a hydrocarbyl derivative of ammonia. It is one of the most basic organic chemical materials, and it is mainly used in vulcanization accelerators, medicines, insect repellent products, printing an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10B01D53/84B01D53/54C02F3/34C12R1/085
CPCB01D53/54B01D53/84B01D2251/95B01D2257/40B09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/085Y02P20/59Y02A50/20
Inventor 李云飞安太成李世蔼李桂英陈江耀
Owner 紫科装备股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com