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Pseudomonas aeruginosa with methylamine degrading capability and application thereof

A technology of Pseudomonas aeruginosa and methylamine, which is applied in the field of microorganisms and can solve problems such as no degradation of methylamine by Pseudomonas aeruginosa.

Active Publication Date: 2017-12-08
紫科装备股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature research, so far, there have been no relevant reports and patents on the use of Pseudomonas aeruginosa to degrade methylamine.

Method used

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  • Pseudomonas aeruginosa with methylamine degrading capability and application thereof
  • Pseudomonas aeruginosa with methylamine degrading capability and application thereof
  • Pseudomonas aeruginosa with methylamine degrading capability and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A strain of Pseudomonas aeruginosa with the ability to degrade methylamine. The name of the strain is Pseudomonas aeruginosa (Pseudomonas aeruginosa) GDUTAN1. It is preserved in the China Center for Type Culture Collection, and the preservation address is Bayi, Wuchang District, Wuhan City, Hubei Province Wuhan University Campus, No. 299 Road; the preservation number is CCTCC NO: M 2017283, and the preservation date is May 24, 2017.

[0031] The Pseudomonas aeruginosa GDUTAN1 in this example was isolated and screened from a landfill leachate in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: the acclimatization medium used is inorganic salt medium g / L (every 1000 mL inorganic salt medium contains: K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, 1mL trace element solution (g / L): CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl ...

Embodiment 2

[0051] This example is the application of Pseudomonas aeruginosa GDUTAN1 in environmental restoration, which can degrade methylamine in the environment. The environment includes the atmosphere, water body or soil.

[0052] The method that the methylamine degrading ability of the Pseudomonas aeruginosa (Pseudomonas aeruginosa) GDUTAN1 that the present invention screens obtains is tested and verified below is as follows:

[0053] Inoculate the Pseudomonas aeruginosa GDUTAN1 strain preserved on the slant into the LB enriched culture medium, activate the bacteria in a shaker at 35°C and 150 rpm for 24 hours, centrifuge the bacteria liquid, and collect the bacteria. And use 5mL inorganic salt solution (every 100mL inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.12g, KH 2 PO 4 0.12g, NH 4 Cl 0.04g, MgSO 4 ·7H 2 O 0.02g, FeSO 4 ·7H 2 O0.001g, CaCl 2 2H 2 O 0.02g, MnSO 4 4H 2 O 0.02g, CuSO 4 2H 2 O 0.001g, ZnSO 4 ·7H 2 O 0.02g, CoCl 2 ·6H 2 O 0.009g, Na 2 M...

Embodiment 3

[0055] Inoculate the Pseudomonas aeruginosa GDUTAN1 strain preserved on the slant into the LB enriched culture medium, activate the bacteria in a shaker at 35°C and 150 rpm for 24 hours, centrifuge the bacteria liquid, and collect the bacteria. And resuspend with 5mL inorganic salt solution, inoculate 2.0mL bacterial liquid in the 100mL inorganic salt solution (same as embodiment 2) containing 50mg / L methylamine, wherein the pH value of inorganic salt is 8, shake table reaction at 35 ℃ 100rpm After 96 hours, samples were taken regularly to determine the degradation rate by spectrophotometry. The determination of the degradation rate is the same as in Example 1, and the degradation rate is 41.5%.

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Abstract

The invention discloses a pseudomonas aeruginosa with methylamine degrading capability and an application thereof. The name of the bacterial strain is pseudomonas aeruginosa GDUTAN1; the bacterial strain is collected at China Center for Type Culture Collection; the collection address is Wuhan University in Wuhan City of China; the collection number is CCTCC NO: M2017283; the collection date is on May 24th, 2017. The pseudomonas aeruginosa GDUTAN1 is gram negative and is of ball rod shape; the colonial morphology is of circular shape; the pseudomonas aeruginosa GDUTAN1 is green and is not transparent; the colony diameter is 1-2mm. The pseudomonas aeruginosa GDUTAN1 disclosed by the invention is used for repairing the environment, is capable of degrading methylamine in the environment and is high in degrading rate. The degrading rate can reach up to 99% or above after 96h degradation under the condition of concentration of substrate of 50-140mg / L.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a strain of Pseudomonas aeruginosa with methylamine degrading ability and application thereof. Background technique [0002] Methylamine (Monomethylamine, MMA, CH 3 NH 2 ) is an aliphatic amine odor compound with the simplest molecular structure. It has the characteristics of low olfactory threshold and difficult biochemical degradation. Its olfactory threshold is as low as 0.021ppm, and its vapor pressure is 202.65kPa at 25°C. It can produce a strong irritating fish odor at a low concentration, and is easily absorbed by the skin, respiratory tract and gastrointestinal tract, etc., has a strong stimulating and corrosive effect on the eyes, skin and respiratory tract mucous membrane, and has a harmful effect on the whole body. Sympathomimetic effect. At the same time, methylamine is also an organic chemical raw material, which is used in all walks of life...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C02F3/34B09C1/10C12R1/385C02F101/38
CPCB01D53/72B01D53/84C02F3/34B09C1/10C02F2101/38B01D2251/95B01D2257/704C12R2001/385C12N1/205Y02A50/20B09C1/00B09C2101/00B09C1/105B01D2257/90C12N1/20
Inventor 安太成李世蔼李桂英
Owner 紫科装备股份有限公司
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