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Method for mineralizing heavy metals in leachate of garbage burning bottom ash by Lysinibacillus fusiformis

A technology of Bacillus lysinum and waste incineration, applied in chemical instruments and methods, bacteria, water pollutants, etc., can solve problems such as soil and groundwater pollution, reach the scope of application without secondary pollution, obvious effect, and easy operation Effect

Active Publication Date: 2019-04-12
ZHEJIANG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of method utilizing spindle-shaped lysine bacillus to mineralize heavy metals in the leachate of the bottom ash of garbage incineration, and to solve the problem of heavy metals in the leachate produced by the bottom ash of garbage incineration due to natural precipitation and the like. and groundwater pollution

Method used

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  • Method for mineralizing heavy metals in leachate of garbage burning bottom ash by Lysinibacillus fusiformis
  • Method for mineralizing heavy metals in leachate of garbage burning bottom ash by Lysinibacillus fusiformis
  • Method for mineralizing heavy metals in leachate of garbage burning bottom ash by Lysinibacillus fusiformis

Examples

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Effect test

Embodiment 1

[0022] Example 1 Study on the adaptability of Bacillus lysinus spindle-shaped in the bottom ash leachate of garbage incineration Sodium chloride is 5g / L, and the solvent is made of garbage incineration bottom ash leachate and deionized water, pH8.5±0.1.

[0023] Spindle-shaped lysine bacillus was inoculated into the nutrient broth medium prepared by making solvents from waste incineration bottom ash leachate and deionized water according to different volumes. The volume concentration gradient of waste incineration bottom ash leachate in the solvent was: 0%, 1%, 2.5%, 5%, 10%, 25%, 50%, 100%. Cultivate overnight (12h) in a shaker at 30°C and 130rpm, through the growth curve of the spindle-shaped lysine bacillus in different concentrations of waste incineration bottom ash leachate (using OD 600 express) see figure 1 As shown, the results showed that Lysinibacillus spindle-shaped can grow normally in the waste incineration bottom ash leachate.

Embodiment 2

[0024] Example 2 Spindle-shaped lysinyl bacillus mineralized waste incineration bottom ash leachate

[0025] (1) Carry out enrichment culture on spindle-shaped lysine bacillus

[0026] Spindle-shaped lysine bacillus was inoculated into the nutrient broth medium prepared with the leachate of the bottom ash of garbage incineration as a solvent, and cultured overnight (12h) in a shaker at 30°C and 130rpm to obtain a bacterial solution, which was passed through the garbage Dilution of incineration bottom ash leachate to adjust OD of bacterial solution 600 is 1.0;

[0027] The formula of the nutrient broth medium is: 1L waste incineration bottom ash leachate, 10g peptone, 1.5g yeast extract powder, 1.5g beef extract and 5g sodium chloride, and the pH is 8.5±0.1.

[0028] (2) Mineralization of waste incineration bottom ash leachate with Bacillus spindle-shaped lysinus

[0029] In the bacterium liquid that 1000ml step (1) obtains, add 20g urea, 2.776g (25mmol) calcium chloride, ob...

Embodiment 3

[0035]The pH in Experimental Example 2 was changed to 4.0±0.1, 12.0±0.1, and other operations were the same as in Example 2. The removal rates of Pb, Cd and As in the waste incineration bottom ash leachate are shown in Table 1.

[0036] Table 1 Removal rate of heavy metals at different pH in the mineralization repair of Bacillus spindle-shaped lysinus

[0037]

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Abstract

The invention discloses a method for mineralizing heavy metals in a leachate of garbage burning bottom ash by Lysinibacillus fusiformis. Lysinibacillus fusiformis is inoculated into the garbage burning bottom ash leachate containing urea and calcium chloride, shaking culture in a shaker at 20 to 30 DEG C at a shaking speed of 100 to 150 rpm is carried out, so that mineralization of heavy metals inthe garbage burning bottom ash leachate is realized. According to the invention, through repairing and treatment of the heavy metals in the garbage burning bottom ash leachate by the Lysinibacillus fusiformis under the conditions of urea and calcium chloride, the method has the advantages of simple process, convenient operation, obvious effect, low treatment cost, wide application range, no secondary pollution and other advantages. Analysis on the repaired leachate after degradation by ICP-OES shows that the removal rates of Pb, Cd and As are 96.0%, 98.9% and 92.8%, respectively.

Description

(1) Technical field [0001] The invention relates to a method for mineralizing and treating garbage incineration bottom ash leachate by using Spinosa lysinus bacillus, and belongs to the technical field of environmental protection solid waste treatment. (2) Background technology [0002] With the development and improvement of our society, since the 1980s, the government has also been committed to the construction of domestic waste incineration plants. By the end of 2012, 213 waste incineration plants have been built nationwide, and the daily waste processing capacity of waste incineration plants is 215844t / d. The bottom ash produced by waste incineration contains various heavy metal components (such as cadmium, lead, arsenic, etc.). A large amount of waste incineration bottom ash will produce a large amount of heavy metal leachate under natural conditions such as rainfall, resulting in serious secondary pollution. [0003] Lead is a common heavy metal that is toxic. Lead ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C02F101/10C02F101/20
CPCC02F3/34C02F2101/103C02F2101/20C12N1/20
Inventor 潘响亮赵瑶瑶张世杰
Owner ZHEJIANG UNIV OF TECH
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