Method for inhibiting algae growth by coupling modified clay with algal inhibition bacteria

An algae and clay technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of a large amount of algae-lysing bacteria and difficult to meet the actual environmental governance, etc. Prepare simple effects

Active Publication Date: 2018-09-21
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Application Information

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Problems solved by technology

However, in the face of the population dominance of algae in the red tide phenomenon, the demand for alga

Method used

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  • Method for inhibiting algae growth by coupling modified clay with algal inhibition bacteria
  • Method for inhibiting algae growth by coupling modified clay with algal inhibition bacteria
  • Method for inhibiting algae growth by coupling modified clay with algal inhibition bacteria

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preparation example Construction

[0048] The preparation method of modified clay: take 100mg of the above two modifiers, add 10mL of 1% dilute hydrochloric acid solution, stir or shake to dissolve the modifier, and then add 100mL of filtered seawater to obtain 1mg / mL modifier hydrochloric acid Solution, then weigh 1g of sodium montmorillonite, and add 100mL of modifying agent hydrochloric acid solution while stirring to configure the modified clay.

[0049] Isolation and Screening of strains The samples were from algae symbiotic microorganisms, which were cultivated by Shenzhen Graduate School of Tsinghua University. The strain cultivation method used LB medium. The algae microbes came from the red tide samples that broke out in Tung Chung, Shenzhen.

[0050] LB solid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, deionized water 1000ml, agar powder 15g, pH7.0.

[0051] LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, deionized water 1000ml, pH7.0.

[0052] Solid LB cultur...

example 1

[0054] Example 1, the selection of tested algae species

[0055] In the present invention, we have selected the red tide algae that is common in the sea areas of Guangdong --- Scrippsiella trochoidea (Scrippsiella trochoidea, cell pear shape, 16-36 μm long, 20-23 μm wide), which belongs to dinoflagellates and is widely distributed in Near shore and estuaries, explosive proliferation can cause local hypoxia in water bodies, bringing harm to marine organisms and aquaculture. From 2010 to the present, red tides of different scales have occurred in the coast of Guangdong for seven consecutive years, and it has become a high-frequency species of red tides. Taking August 12th to August 30th, 2013 as an example, a large-scale red tide of Steinerella conicum occurred in the area of ​​Baguang and Dongchong-Yantian Port in Shenzhen, which lasted for 19 days and had a maximum area of ​​15 square kilometers. Therefore, the tested species selected in this invention is Steineria coniformis...

example 2

[0056] Example 2, the preparation of modified clay

[0057] After comparing several clay types reported in the literature, we chose sodium montmorillonite. Montmorillonite is a three-layer structure clay mineral. Due to its lattice defects and surface hydroxyl structure, the surface of this particle is negatively charged in water, but it can be prepared by chelating with sodium ions. Adsorptive power of cationic clay.

[0058] As modifiers, high-efficiency and low-toxic high-alkyl compounds are selected. Different types of modifiers have a great influence on the effect of clay modification. We choose two modifiers with lower toxicity: hexadecyl three Two modifiers, methyl ammonium bromide and tributyl hexadecyl phosphonium bromide.

[0059] Preparation of modified clay: Take 100mg of modifier, add 10mL of 1% dilute hydrochloric acid solution, stir or oscillate to dissolve the modifier, then add 100mL of filtered seawater to obtain 1mg / mL modifier hydrochloric acid solution, ...

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Abstract

The invention provides a method for inhibiting algae growth by coupling modified clay with algal inhibition bacteria. The modified clay is cetyl trimethyl ammonium bromide modified sodium montmorillonite. The algal inhibiting bacteria can be lysinibacillus fusiformis J8 which is called a J8 strain for short. The J8 strain is preserved in the Guangdong microbial culture collection center on December 13th, 2017, and the preservation number is GDMCC NO. 60298. Experimental data shows that the combined application of the modified clay and the algal inhibiting bacteria (J8) has an obvious inhibition effect on algal cells, the continuous algal inhibition time can reach 168 hours, and the rebound of the number of the algae cells is avoided. The modified clay used in the method is wide in source,simple to prepare, low in cost and easy to operate; the modified clay and the algal inhibiting bacteria are combined so that the red tide can be more thoroughly inhibited; a method for inhibiting thered tide by combining the modified clay and the algal inhibition bacteria, no secondary pollution is generated, and the method is an environment-friendly method.

Description

technical field [0001] The invention belongs to the field of marine environment protection, and in particular relates to a method for inhibiting algae growth through the coupled use of modified clay and algastatic bacteria. Background technique [0002] Red tide is one of the serious global marine natural disasters, which has attracted great attention from all over the world. In recent years, the pollution of my country's coastal waters has intensified, the problem of eutrophication in some sea areas has become prominent, and red tide outbreaks have become increasingly frequent, which has brought serious ecological, resource and environmental problems to coastal waters, and caused huge economic losses. A major threat to sustainable economic development. Therefore, it is one of the major problems to be solved urgently in the field of red tide prevention and control to study new efficient and environmentally friendly red tide control methods. [0003] Although some red tide c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2303/20C12N1/205C12R2001/01
Inventor 周进蔡中华吴凡孙景云孟繁蔷
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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