Method for mineralizing and fixing heavy metals in tailings by using lysinibacillus fusiformis and quicklime

A lysine bacillus, spindle-shaped technology, applied in the field of environmental engineering, can solve problems such as heavy metal pollution in tailings, achieve the effect of reducing the possibility of environmental pollution, wide sources, and low price

Pending Publication Date: 2020-08-14
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the heavy metal pollution of tailings and further improve the effect of microbial calcification and fixation of heavy metals, the purpose of the present invention is t...

Method used

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  • Method for mineralizing and fixing heavy metals in tailings by using lysinibacillus fusiformis and quicklime
  • Method for mineralizing and fixing heavy metals in tailings by using lysinibacillus fusiformis and quicklime
  • Method for mineralizing and fixing heavy metals in tailings by using lysinibacillus fusiformis and quicklime

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Spindle-shaped lysine bacillus and quicklime are applied to the method for fixing heavy metals in copper-nickel tailings, as follows: the first step: use the nutrient broth medium purchased from the website of Sinopharm Chemical Reagent, and the configuration concentration is 0.018 g / ml liquid medium, sterilized at 121°C for 30 minutes and cooled, then cultured the purchased spindle-shaped lysine bacillus in a shaker at 25°C and 100 rpm for 10 hours to activate, and the activated spindle-shaped lysine Acidic acid bacillus was inoculated into the culture medium and placed on a shaker with a rotation speed of 100rpm to expand culture for 1 day, and the microbial concentration was obtained as OD 600 =1.0-2.0 bacteria solution, the culture condition is temperature 20°C, use 0.018g / ml nutrient broth medium to dilute the bacteria solution concentration to OD before curing tailings 600 =1.0 or 1.2; second step: take by weighing 65g of copper-nickel tailings sample, for eve...

Embodiment 2

[0044] Spindle-shaped lysine bacillus and quicklime are applied to the method for fixing heavy metals in gold tailings, as follows: the first step: use the nutrient broth medium purchased from the website of Sinopharm Chemical Reagent, and the configuration concentration is 0.018g / ml of liquid medium, sterilized at 121°C for 30 minutes and cooled, then cultured the purchased spindle-shaped lysine bacillus in a shaker at 30°C and 150 rpm for 16 hours to activate, and the activated spindle-shaped lysine Acidobacillus was inoculated into the culture medium and placed on a shaker with a rotation speed of 150rpm to expand the culture for 3 days, and the microbial concentration was obtained as OD 600 =1.0-2.0 bacteria solution, culture condition is temperature 30°C, use 0.018g / ml nutrient broth medium to dilute the concentration of bacteria solution to OD before curing tailings 600 =1.0 or 1.5; second step: take by weighing 65g of gold tailings sample, for every 65g of tailings...

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Abstract

The invention discloses a method for mineralizing and fixing heavy metals in tailings by using lysinibacillus fusiformis and quick lime. The quick lime is used for improving the effect of mineralizingand fixing the heavy metals by microorganisms, the quick lime can generate calcium ions and hydroxyl ions when reacting with water, the generated calcium ions can provide a calcium source required bycalcification of the microorganisms, and the hydroxyl ions can improve the pH of a tailing solidification system; the lysinibacillus fusiformis simultaneously cement tailing particles with the quicklime while generating more calcium carbonate crystals to fix the heavy metals, so that the concentration of lead ions, copper ions, zinc ions and nickel ions in tailing leachate is reduced, the possibility that heavy metals in the tailings are leached and flow into nearby soil and rivers is greatly reduced, and large-scale environmental pollution is avoided; and meanwhile, a solidified body has certain compressive strength, so that a feasible idea is provided for resource utilization of the tailings.

Description

technical field [0001] The invention relates to the technical field of environmental engineering, in particular to the solidification of non-ferrous metal fine-grained tailings, and relates to a method for mineralizing and fixing heavy metals in tailings by using spindle-shaped lysine bacillus and quicklime. Background technique [0002] Mineral resources are considered to be the material basis for national development and human survival. During the development and utilization of mineral resources, industrial waste tailings will be produced. The tailings contain a large amount of leaching heavy metals. After a large amount of tailings are produced, the time goes by It will lead to a series of environmental problems, which have become the bottleneck of my country's mining economic development and the sustainable development of mining cities, and pose a certain threat to human health and safety. [0003] The use of microbial-induced carbonate precipitation technology to treat h...

Claims

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Application Information

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IPC IPC(8): C22B3/18C22B11/00C22B23/00C22B15/00C22B13/00C22B19/20C22B19/30C12N1/20C12R1/01
CPCC12N1/20C22B3/18C22B11/042C22B13/045C22B15/0065C22B19/20C22B19/30C22B23/0407Y02P10/20
Inventor 张道勇王文艺王潇男潘响亮
Owner ZHEJIANG UNIV OF TECH
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