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Method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment

A technology of algae-lysing bacteria and Microcystis green, applied in the directions of microorganism-based methods, bacteria, chemical instruments and methods, etc., can solve the problems of unscaled application, in the laboratory stage, etc., and achieves low cost, algae-lysis The effect is ideal and the application prospect is broad

Inactive Publication Date: 2014-01-08
CHANGZHOU UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] At present, experts and scholars at home and abroad generally believe that the biological method is the most effective, safe and economical method to solve the problem of cyanobacteria blooms. Microorganisms that dissolve or infect cyanobacteria cells mainly belong to the genus Pseudomonas, Bacillus, etc., and will be collectively referred to as algicidal bacteria. However, the research on algicidal bacteria is still in the laboratory stage and has not been applied on a large scale. In actual engineering, the systematic research on the algicidal characteristics of algae-dissolving bacteria, the search for the best algae-dissolving conditions for bacteria, and the exploration of bacterial algae-dissolving methods can provide a certain technical basis for the biological control of cyanobacteria blooms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] In this example, 100 mL of fresh Microcystis aeruginosa liquid 1 was treated, and the concentration of chlorophyll a was 202.11 mg / m 3 , pH value is 7.2. The specific implementation steps are as follows: first, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130 r / min until the logarithmic phase (16 h), and then, according to the bacteria-to-algae ratio of 1:2, the 50 mL of logarithmic phase bacterial liquid was inoculated into algae liquid, and sterile water was used as blank control, and the initial chlorophyll a concentration was determined to be 168.42 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the concentration of chlorophyll a was 8.68mg / m at 96 h 3 , the removal rate is 94.85%, which can dissolve algae cells well.

Embodiment 2

[0056] What this embodiment deals with is 100 mL of fresh Microcystis aeruginosa liquid 2, and the concentration of chlorophyll a is 220.93 mg / m 3 , pH value is 7.2. The specific implementation steps are as follows: firstly, the Bacillus lysinica was cultured to the logarithmic phase (16 h) at a temperature of 30 °C and a shaker speed of 130 r / min, and then the bacteria were injected at a bacterial ratio of 1:5. 20 mL of the logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control, and the initial chlorophyll a concentration was determined to be 189.62 mg / m 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the chlorophyll a concentration was 21.6 mg / m at 96 h 3 , the removal rate reached 88.61%, and the algae-dissolvin...

Embodiment 3

[0058] In this example, 100 mL of fresh Microcystis aeruginosa liquid 3 was treated, and the concentration of chlorophyll a was 235.88 mg / m 3 , pH value is 7.2. The specific implementation steps are as follows: First, culture Bacillus lysinus at a temperature of 30°C and a shaker speed of 130 r / min until the logarithmic phase (16 h), and then, according to the bacteria-to-algae ratio of 1:10, the 10 mL of logarithmic phase bacterial liquid was inoculated into the algae liquid, and sterile water was used as a blank control, and the initial chlorophyll a concentration was determined to be 199.22 mg / m by sampling 3 , placed in a light incubator at 28°C, light intensity 2500 lux, and light cycle 12 h: 12 h for static culture, and samples were taken every 24 h to determine the concentration of chlorophyll a. After the algae liquid treated by the above method, the concentration of chlorophyll a was 25.28 mg / m at 96 h 3 , the removal rate reached 87.31%, and the algae-dissolving ef...

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Abstract

The invention relates to a method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment, belonging to the technical field of environment-friendly water treatment. The invention provides Lysinibacillus fusiformis of which the collection number is CGMCC NO.7520. The invention also provides a method for screening algicidal bacteria and removing microcystis aeruginosa from Lake Tai branch river sediment. The screened algicidal bacteria has a certain dissolution action on the dominant alga strain microcystis aeruginosa in blue algae bloom, and the algicidal bacteria TR3 can be obtained by separation. Under the optimum conditions, the removal rate of the algicidal bacteria TR3 for microcystis aeruginosa within 96 hours can reach 97.18%, so the algicidal bacteria TR3 has very ideal alga dissolution effect; and thus, the algicidal bacteria TR3 can better display the alga dissolution characteristic in a natural water body, and has wider application prospects in large-area water body blue algae bloom pollution treatment.

Description

technical field [0001] The invention belongs to the technical field of environmental protection water treatment, and in particular relates to an algae-dissolving bacterium screened from the bottom mud of a branch of the Taihu Lake Basin and a method for removing Microcystis aeruginosa by using the same. Background technique [0002] my country's fresh water resources are limited, and the increasingly serious water pollution problem has exacerbated the shortage of fresh water resources in my country. The outbreak of cyanobacteria has caused great harm to the aquatic ecosystem, fishery resources and ecological landscape. With the frequent occurrence of cyanobacteria outbreaks, the problem of algal bloom pollution has gradually been paid attention to by people. The release of algal toxins has become the research direction of many scholars. Exploring the prevention and control of cyanobacteria outbreaks and their derived algal toxin pollution is related to the safety of human li...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 张文艺李仁霞陈雪珍冯国勇陈晶
Owner CHANGZHOU UNIV
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