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Preparation and application of netropsin lysinibacillus fusiformis selenium-rich inoculant

A lysine bacillus and spindle-shaped technology is applied in the field of preparation of a selenium-enriched inoculum of spindle-shaped bacillus lysine, and can solve the problems of low selenium enrichment ability and the like

Active Publication Date: 2017-06-23
ANHUI TECH BANK BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, the selenium enrichment ability of these bacteria is far lower than the lysine bacillus of the present invention
There is no literature report on selenium-enriched Lysinibacillus fusiformis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of spindle-shaped lysine bacillus selenium-enriched bacterial agent:

[0026] (1) Add 5g of beef extract, 10g of peptone, 5g of NaCl, 120mg of sodium selenite and 1000ml of distilled water to the container in sequence, adjust the pH value to 7.0-7.2, and sterilize with high pressure steam at 121°C for 20 minutes to obtain a liquid medium;

[0027] (2) Get the bacterial classification of spindle-shaped lysine bacillus, inoculate it in the liquid medium in the step (1), cultivate it on a shaker at 28°C for 48h, and obtain the fermentation liquid of spindle-shaped lysine bacillus rich in selenium ;

[0028] (3) Centrifuge the fermented liquid in the step (2), collect the thallus, and blow dry at 85-95° C. to obtain the spindle-shaped lysinic bacillus selenium-enriched inoculum, and use the selenium-enriched inoculum The selenium content in the prepared bacterial powder is 3000mg / kg.

Embodiment 2

[0030] Toxicological experiments on the selenium-enriched bacterial agent of Bacillus spindle-shaped lysinus:

[0031] Feed the 10-day-old healthy suckling pigs with 10g and 100g of each lysine-enriched bacterial agent, respectively, for 5 consecutive days, and observe the spirit, appetite, defecation, and Whether the piglets have any toxic effect and death phenomenon.

[0032] Results: The 10-day-old healthy suckling pigs were fed with the selenium-enriched bacterial agent of Bacillus fusiformis for 5 consecutive days and observed for 10 days. Toxic effects and death on suckling pigs indicate that the bacterial agent is safe.

Embodiment 3

[0034] Effects of selenium-enriched bacterial agent of Lysinus spindle-shaped bacteria on the selenium content of pork:

[0035] Purpose and Significance: To select weaned piglets as the test object, to investigate the effect of the selenium-enriched bacterial agent of Bacillus fusiformis on the selenium content of pork under the condition of using Tianbang brand feed and without antibiotics.

[0036] Test animals: 21-day-old weaned piglets

[0037] Animal Management

[0038] The experimental weaned piglets were raised in the experimental pig farm with natural ventilation and good environmental sanitation conditions. Free feeding, drinking water, deworming and immunization were handled according to the pig farm regulations. Animal feeding and health conditions were observed at any time. All diarrheal pigs were left untreated. From the age of 21 days, each pig in the experimental group was fed with 10 g of the selenium-enriched bacterial agent of Lysinica spindle-shaped per ...

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Abstract

The invention provides preparation and application of a netropsin lysinibacillus fusiformis selenium-rich inoculant, and relates to the field of organic selenium preparations for animal breeding. The netropsin lysinibacillus fusiformis is able to enrich inorganic selenium with high concentration, and mycoprotein per 10 kg (dry weight) is able to enrich 20,000 mg selenium in total; inoculant powder prepared through the produced selenium-rich inoculant containing selenium-enriched protein contains more than 3,000 mg / kg of selenium in total and can be directly fed to animal to supplement selenium element, thus the meat quality of the bred animal can be improved; toxicity experiment verifies that netropsin lysinibacillus fusiformis does not cause adverse reaction in tested animal; the netropsin lysinibacillus fusiformis can be used for producing phytase, protease and glycosidase, so that the digestion of the animal can be improved, and the feed utilization rate is increased.

Description

technical field [0001] The invention relates to the field of organic selenium preparations for animal breeding, in particular to the preparation and application of a selenium-rich bacterial preparation of Bacillus spindle-shaped lysinus. Background technique [0002] Selenium is one of the trace elements necessary for normal physiological activities of humans and animals. Studies have found that selenium has the physiological function of improving human immunity, can resist toxic heavy metals in organisms, and has anti-oxidation, anti-cancer, detoxification, and heart protection. The role of the liver. At the same time, selenium also has a certain preventive effect on more than 40 diseases such as cataract cardiovascular disease, Keshan disease, Kashin-Beck disease, and arthritis. [0003] Selenium exists in the form of inorganic selenium and organic selenium. Since the distribution of selenium in nature is low and unbalanced, and inorganic selenium has certain toxic and si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/07
CPCC12N1/20
Inventor 张邦辉胡传炯张东武聂敏
Owner ANHUI TECH BANK BIO TECH
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