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Lysinibacillus fusiformis for degrading dairy cow faeces sour matter and application of lysinibacillus sphaericus

A technology of Bacillus lysine and dairy cow dung, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of a large number of manpower and equipment investment and consumption, and achieve the improvement of biomass and catalytic performance, strong growth adaptability, The effect of high resistance to organic acids

Active Publication Date: 2017-11-14
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the traditional centralized treatment method is used for manure treatment, it is necessary to wash the manure with water, and then process it through solid-liquid separation, anaerobic fermentation, composting, etc. This method requires a lot of manpower, water and electricity, equipment investment and consumption

Method used

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  • Lysinibacillus fusiformis for degrading dairy cow faeces sour matter and application of lysinibacillus sphaericus
  • Lysinibacillus fusiformis for degrading dairy cow faeces sour matter and application of lysinibacillus sphaericus

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Enrichment culture and isolation and purification of the lysine bacillus LF-N1 of embodiment 1

[0025] Take 0.2g of dairy cow manure accumulation and inoculate it into 100mL liquid screening medium. The medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, 5g / L dry cow manure, acetic acid , propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, and isovaleric acid are each 1g / L, and 0.1g / L indole and 0.1g / L skatole, adjust the pH to 7.0; culture 37 ℃, 200r·min -1 Shaker culture 48h. Then take 100 μL of the bacterial solution from the above-mentioned culture solution, inoculate it into a new liquid screening medium (the formula of the medium is the same as above), and repeat the above-mentioned cultivation operation 2 to 3 times. Then get 100 μ L bacterium liquid from final culture liquid, inoculate in acclimatization medium and carry out acclimatization culture, the formula of described acclimatization medium is: tryptone 10g / L, yeas...

Embodiment 2

[0026] Scanning electron microscope observation, identification and species analysis of embodiment 2 bacterial strains

[0027] The isolated and purified monoclonal Bacillus lysinica LF-N1 was inoculated into 50mL liquid LB medium, and the medium formula was: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, take 5μL The liquid sample is used to prepare the scanning electron microscope sample. In the imaging photo with a magnification of 10,000x, the morphology of the lysine bacillus LF-N1 is as follows figure 1 The rod shape shown.

[0028]Get the culture fluid of 1.5ml fresh Bacillus lysinica LF-N1, centrifuge 12,000g, 1min, remove supernatant, use the solution provided in the genomic DNA extraction kit (purchased from Axygen Company) of cell pellet Resuspend, and extract genomic DNA according to the operating instructions of the kit, and finally dissolve the DNA pellet in 100 μL TE buffer (10mM Tris, 1mM EDTA, pH8.0), and dilute the genomic DNA to 20mg / L, take 1μL...

Embodiment 3

[0030] Example 3 Analysis of the Degradation Effect of Organic Acid Odor by Lysinibacillus LF-N1

[0031] Inoculate the single colony Bacillus lysinica LF-N1 on the LB plate into 50mL liquid screening medium, the medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, 5g / L dry cow dung, adjust the pH to 7.0, add 1g / L acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isovaleric acid, and 0.1g / L ind Indole and skatole. Cultivate on a shaker at 37°C and 200r min-1 for 24 to 48 hours, then inoculate all the culture solution into 1000ml liquid screening medium. The medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, 5g / L dry cow dung, additionally containing 0.1g / L indole and skatole, and 1g / L or 2g / L acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isoamyl acid, adjust the pH to 7.0; 37°C, 200rpm, shaking culture for 24-192h, sample 1ml regularly, get the supernatant by cent...

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Abstract

The invention discloses a lysinibacillus fusiformis for degrading a dairy cow faeces sour matter and application of the lysinibacillus fusiformis. Lysinibacillus fusiformis LF-N1 provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) now with a preservation number being CCTCC No: M 2017289. The lysinibacillus fusiformis LF-N1 can effectively degrade short-chain sour fatty acid compounds contained in biomass substrates including sawdust when being therein, is suitable for preparing an efficient microbial agent product in a dairy cow breeding bed ecological breeding mode, and can also efficiently degrade two organic pollutants, including indole and skatole in livestock and poultry industry, thus having very good application prospect in the aspects of ecological breeding, environmental governance and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental microbe application, and in particular relates to a lysinic bacillus for degrading sour and odorous feces of dairy cows and an application thereof. Background technique [0002] my country's livestock and poultry breeding industry is gradually moving towards scale and intensification, and its breeding mode and breeding technology have been greatly improved. Factors such as land use restrictions and other factors have brought about various environmental pollution problems, causing the largest source of agricultural non-point source pollution to become aquaculture pollution, and prompting agricultural pollution emissions to gradually exceed industrial non-point source emissions in recent years. [0003] The amount of feces and sewage treatment in the process of dairy farming is particularly large. If a large dairy farm has 10,000 dairy cows in stock, it can produce 600 tons of feces and urine p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C02F103/20C12R1/07
CPCC02F11/02C02F2103/20C12N1/20C12N1/205C12R2001/07
Inventor 孙俊松赵利超余从田吴海英史吉平姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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