Lysinibacillus fusiformis and microbial agent, as well as applications thereof

A technology of lysine bacillus and microbial agent, applied in the field of agricultural biology, can solve the problems of wasting limited resources, low content of available phosphorus, and low utilization rate of phosphorus in the current season, and achieve the effect of increasing fertilizer efficiency

Active Publication Date: 2014-07-30
YANTAI DIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing various types of traditional phosphorus fertilizers are easily fixed by the soil after being applied to the soil, and the available phosphorus content is low. According to statistics, the utilization rate of phos

Method used

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  • Lysinibacillus fusiformis and microbial agent, as well as applications thereof
  • Lysinibacillus fusiformis and microbial agent, as well as applications thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] Wherein, the preparation method of the microbial bacterial agent may include: inoculating the spindle-shaped lysine bacillus of CGMCC No. 8835 in the fermentation medium for culturing. Preferably, in each gram of the microbial agent obtained by culturing, the number of living bacteria of the spindle-shaped lysine bacillus whose preservation number is CGMCC NO.8835 can be (5-200)×10 7 CFU, more preferably (20-120)×10 7 CFU. Wherein, the cultivation temperature may be 28-35° C., and the cultivation time may be 10-50 hours.

[0022] Preferably, the preparation method of the microbial agent may also include: mixing the material obtained after culturing in the fermentation medium with an inert solid carrier and a binder; the inert solid carrier includes clay, silicon dioxide, silicon At least one of algal earth, kaolin, attapulgite, montmorillonite, bentonite, talc, white carbon black and calcium carbonate; the binder includes maltose and / or dextrin. Preferably, the amoun...

Embodiment 1

[0029] This example is used to illustrate the cultivation of the spindle-shaped lysine bacillus of the present invention and the preparation of the microbial inoculum.

[0030] The preservation number is the spindle-shaped lysine bacillus (Lysinibacillus fusiformis) of CGMCC NO.8835 inoculated in the LB medium (containing the tryptone of 10g / L, the yeast extract of 5g / L and the NaCl of 10g / L), Cultivate at 30°C and shake at 200 rpm until the bacterial density is OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0031] 100mL of above-mentioned seed bacterium liquid is added in 100L fermentation medium (fermentation medium contains the soybean powder of 80g / L, the starch of 80g / L, the sucrose of 80g / L, the soybean oil of 40g / L, the sodium chloride of 3g / L, 3g / L of calcium carbonate, 5g / L of potassium dihydrogen phosphate and 5g / L of ferrous sulfate), cultured at 30°C, samples were taken during the culture and observed by direct counting under a microscop...

Embodiment 2

[0033] This example is used to illustrate the cultivation of the spindle-shaped lysine bacillus of the present invention and the preparation of the microbial inoculum.

[0034] The preservation number is the spindle-shaped lysine bacillus (Lysinibacillus fusiformis) of CGMCC NO.8835 inoculated in the LB medium (containing the tryptone of 10g / L, the yeast extract of 5g / L and the NaCl of 10g / L), Cultivate at 30°C and shake at 200 rpm to bacterial density OD600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0035] 100mL of above-mentioned seed bacterium liquid is added in 100L fermentation medium (fermentation medium contains the soybean powder of 80g / L, the starch of 80g / L, the sucrose of 80g / L, the soybean oil of 40g / L, the sodium chloride of 3g / L, 3g / L of calcium carbonate, 5g / L of potassium dihydrogen phosphate and 5g / L of ferrous sulfate), cultured at 30°C, samples were taken during the culture and observed by direct counting under a microscope until each...

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Abstract

The invention provides lysinibacillus fusiformis, the collection number of which is CGMCC (China General Microbiological Culture Collection Center) NO.8835. The invention further provides a microbial agent which contains thalli and a culture medium, wherein the thalli comprises the lysinibacillus fusiformis the collection number of which is CGMCC NO.8835. The invention further provides applications of the lysinibacillus fusiformis and the microbial agent in preparing a microbial fertilizer. Through the technical scheme, the fertilizer efficiency of a phosphorous fertilizer can be more effectively increased.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a spindle-shaped lysine bacillus, a microbial agent and applications thereof. Background technique [0002] Phosphorus fertilizer can promote tomato flower bud differentiation, early flowering and fruiting, promote seedling root growth and improve fruit quality. When phosphorus is deficient, the young shoots and roots grow slowly, the plants are short, the leaves are dark green, dull, and the back is purple. Phosphorus is usually taken up by plants in the form of orthophosphate (hydrogen phosphate or dihydrogen phosphate). When phosphorus enters the plant, most of it becomes organic matter, and a part remains in the form of inorganic salt. Phosphorus exists in the form of phosphate in sugar phosphate, nucleic acid, nucleotide, coenzyme, phospholipid, phytic acid, etc. Phosphorus plays a key role in the reaction of ATP, and phosphorus plays an important role in carbohy...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/01
Inventor 吕昭辉阮志勇赵秉强孔德龙赵君才赵文勇刘慧宋昭王彦伟刘小飞
Owner YANTAI DIYUAN BIOTECH
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