Application of lysinibacillus fusiformis in removing hexavalent chromium in eutrophic wastewater
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A technology of Bacillus lysine and Bacillus, applied in the field of microorganisms, can solve the problems of unproven tolerance and resistance, and achieve the effect of strong adsorption and reduction
Pending Publication Date: 2020-11-13
YANCHENG INST OF TECH +1
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Therefore, the spindle-shaped lysinibacillus WTXJ1-4 with similar genome information can theoretically be screened and cultivated to treat hexavalent chromium pollution or co-contamination of hexavalent chromium and organochlorine, but how can this strain be applied to remove hexavalent chromium in practice? and its biosorption, tolerance and resistance have not yet been proven
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Embodiment 1
[0027] Embodiment 1 Spindle-shaped lysine bacillus to the biosorption-reduction test of Cr(VI)
[0028] 1. Cell preparation
[0029] Take 1000mL LB culture solution of Bacillus spindle-shaped lysinus in the logarithmic phase, centrifuge at 4°C and 13000r / min for 10min, add sterile water and repeat centrifugation for 3 times, and wet the cells with constant weight, as live bacteria, in 4 Store at ℃ for later use.
[0030] 2. Single factor test of biosorption-reduction test of WTXJ1-4 live bacteria
[0031] Take 0.5~3.0g / L WTXJ1-4 live bacteria respectively, add 100mL sterile aqueous solution containing potassium dichromate concentration of 0~500mg / L, at pH2.0~pH7.0, adsorption temperature 26~38℃, Cultivate for 0-24 hours under the condition of rotation speed 0-200r / min, and explore the single-factor suitable conditions for the removal of hexavalent chromium pollutants by WTXJ1-4 living bacteria in eutrophic wastewater. The preset central values of each single factor are as...
Embodiment 2
[0046] Example 2 Tolerance and resistance characteristics of lysine bacillus spindle-shaped to Cr(VI)
[0047] Add concentrations of 0mg / L, 20mg / L, 40mg / L, 60mg / L, 80mg / L, 100mg / L, 200mg / L, 300mg / L, 400mg / L and 500mg / L to 100mL of LB medium respectively. 1 L of potassium dichromate, to which 10mL was added successively in the logarithmic phase spindle-shaped lysine bacillus culture solution, and each concentration was established with 3 parallels, and random sampling was performed at regular intervals to measure the thalline biomass and residual Cr in the solution ( VI) and total Cr, the time relationship curves of the thalline biomass, Cr(VI) removal rate and total Cr removal rate of the spindle-shaped lysine bacillus were established respectively, as Figure 7 shown.
[0048] Depend on Figure 7 It can be seen that with the increase of potassium dichromate concentration, the bacteriostasis of microorganisms rises rapidly first and then tends to be flat, indicating that pot...
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Abstract
The invention discloses application of lysinibacillus fusiformis in removing hexavalent chromium in eutrophic wastewater, and belongs to the technical field of microorganisms. The strain is preservedin China General Microbiological Culture Collection Center; the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.10053, the preservation name is Lysinibacillus fusiformis WTXJ1-4, and the preservation date is November 25, 2014. Through single factor test and orthogonal test optimization, the appropriate biological adsorption conditions of the strain for the hexavalent chromium in the eutrophic wastewater are determined as follows: the pH value is 2.0, the initial concentration of potassium dichromate is 50 mg / L, the adsorption time is 24 hours, the dosage of wet thalli is 1.0 g / L, the adsorption temperature is 34 DEG C, and the rotating speed is 160 r / min. And when the optimized adsorption conditions are balanced, theremoval rates of the WTXJ1-4 living bacteria on the hexavalent chromium and total chromium in the eutrophic wastewater respectively reach 91.6%-94.8% and 42.2%-43.8%. According to the present invention, the Lysinibacillus fusiformis WTXJ1-4 living bacteria have strong adsorption-reduction property, strong tolerance and strong resistance on the hexavalent chromium in the eutrophic wastewater, wherein the action ranges are as follows: the concentration of potassium dichromate is 0-100 mg / L, the concentration of potassium dichromate is 100-300 mg / L, and the concentration of potassium dichromate is 300-500 mg / L.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of spindle-shaped lysine bacillus to remove hexavalent chromium in nutrient-rich wastewater. Background technique [0002] Compared with traditional chemical and physical techniques for removing or recycling metals in waste, biosorption is more economically and environmentally feasible, and has become a promising environmental protection and energy-saving heavy metal sewage (waste) water and waste residue treatment technology. Studies have shown that a variety of microorganisms have good biological removal effects on a variety of single metals or metal combinations. There are many factors that may affect the adsorption efficiency of metal ions on the tested microbial cells. The adsorption mechanism may be due to electrostatic attraction, formation of Surface complexes and chemical interactions between metal ions and functional groups (mainly hyd...
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