Lysinibacillus fusiformis and application thereof in land reclamation and ecological reconstruction in mining area

Abstract

The invention discloses lysinibacillus fusiformis and an application thereof in land reclamation and ecological reconstruction in a mining area. The strain is the lysinibacillus fusiformis, and has acollection number of CGMCC No. 9799. The strain is capable of improving the nutrient content of land reclamation soil in the mining area, and has obvious effects for improving the content of organic matters, alkali-hydrolyzale nitrogen, rapidly available potassium and available phosphorus in the land reclamation soil in the mining area. Moreover, further experiments prove that, the lysinibacillusfusiformis is capable of further improving the biomass of crops, and for culture of wheat or oilseed rapes, the biomass of crops in the land reclamation soil in the mining area can be improved; particularly important, due to use of the lysinibacillus fusiformis, the community structure of soil microorganisms can be effectively and obviously changed, the microbial diversity of the soil is improved,and the quantity of microbes is effectively increased. Therefore, remediation of the microbial community in the soil is accelerated, and the lysinibacillus fusiformis can be widely applied to remediation of the land reclamation soil in the mining area.

Description

technical field

[0001] The invention belongs to the technical field of ecological reconstruction, and relates to a spindle-shaped lysine bacillus and its application in ecological reconstruction of mining area reclamation. Background technique

[0002] Coal plays a decisive role in my country's energy production and consumption, and its proportion has always remained at about 70%. While coal resource mining supports social and economic development, it also has a lasting and serious negative impact on land resources and the ecological environment. The 2007 China Geological Environment Bulletin pointed out that the land area occupied and damaged by mining development in the country was 1.658 million hectares, of which 909,000 hectares were stacked tailings, 522,000 hectares were open pits, 203,000 hectares were subsided by mining, and new damages were added every year. There are about 4 million mu of land, more than 60% of which are arable land or other agricultural land, whic...

Examples

Embodiment 1

[0030] This embodiment illustrates the screening and identification process of spindle-shaped lysine bacillus (Lysinibacillus fusiformis) (CGMCCNo.9799) of the present invention

[0031] 1. Sample Collection

[0032] The soil was collected from a typical reclamation mining area in Shanxi Province, and the 0-20cm soil was collected from the weeds (N 36°28′17.846″, E 113°00′50.383″) in the coal mining subsidence reclamation area of ​​Xishandi Village, Xiangyuan County, Changzhi, Shanxi Province Samples were stored at 4°C; 0-20cm soil samples were collected from the weeds (N 37°08′25.485″, E 111°30′65.453″) of the Ke’e Village dump in Xiaoyi, Shanxi, and stored at 4°C.

[0033] 2. Primary screening of strains

[0034] Weigh 10g of the reclaimed soil in the mining area and add it to 90mL of sterilized 250mL Erlenmeyer flask filled with glass beads and sterile water, shake it under the constant temperature shaker at 30°C and 180r / min for 20min, let it stand for 5min, and absorb 1m...

Embodiment 2

[0071] This embodiment illustrates the preparation of the cultivation of the spindle-shaped lysine bacillus of the present invention and the microbial inoculant

[0072] A spindle-shaped lysine bacillus microbial preparation, which is obtained by expanding the spindle-shaped lysine bacillus and then fully mixing the active ingredient and carrier with a mass ratio of 2:10 to 20, and storing at room temperature;

[0073] The carrier is a solid carrier or a liquid carrier, wherein the solid carrier is a mineral material, a plant material, a polymer compound or an organic material; the mineral material is one or more of clay, kaolin, montmorillonite, zeolite and fly ash several kinds; the plant material is one or more of corn flour, starch, bean flour and peanut flour; the polymer compound is one or two of polyvinyl alcohol and polyglycol; the organic material is One or two of peat, humic acid and organic fertilizer;

[0074] The active ingredient is one or more of living cells o...

Embodiment 3

[0080] This embodiment illustrates the effect of the spindle-shaped lysine bacillus agent of the present invention on improving the nutrient content of the reclaimed soil in the mining area

[0081] Rapeseed rape and spring wheat were planted by the pot method, and the planting time was (60 days, 100 days) respectively. The substrate soil used for potting comes from the reclaimed land of the coal mining subsidence area of ​​Lu'an Group located in the southeast of Shanxi Province, at the western foot of Taihang Mountain.

[0082] Rapeseed soil organic matter content 5.97g / kg, total nitrogen 0.50g / kg, total phosphorus 0.05g / kg, total potassium 20.00g / kg, alkaline nitrogen 42.82mg / kg, available phosphorus 3.39mg / kg, available potassium 98.60mg / kg kg, pH8.29.

[0083] Spring wheat soil organic matter content 6.40g / kg, total nitrogen 0.40g / kg, total phosphorus 0.4g / kg, total potassium 19.80g / kg, alkaline nitrogen 48.76mg / kg, available phosphorus 2.09mg / kg, available potassium 98.5...

technical field

[0001] The invention belongs to the technical field of ecological reconstruction, and relates to a spindle-shaped lysine bacillus and its application in ecological reconstruction of mining area reclamation. Background technique

[0002] Coal plays a decisive role in my country's energy production and consumption, and its proportion has always remained at about 70%. While coal resource mining supports social and economic development, it also has a lasting and serious negative impact on land resources and the ecological environment. The 2007 China Geological Environment Bulletin pointed out that the land area occupied and damaged by mining development in the country was 1.658 million hectares, of which 909,000 hectares were stacked tailings, 522,000 hectares were open pits, 203,000 hectares were subsided by mining, and new damages were added every year. There are about 4 million mu of land, more than 60% of which are arable land or other agricultural land, whic...

Examples

Embodiment 1

[0030] This embodiment illustrates the screening and identification process of spindle-shaped lysine bacillus (Lysinibacillus fusiformis) (CGMCCNo.9799) of the present invention

[0031] 1. Sample Collection

[0032] The soil was collected from a typical reclamation mining area in Shanxi Province, and the 0-20cm soil was collected from the weeds (N 36°28′17.846″, E 113°00′50.383″) in the coal mining subsidence reclamation area of ​​Xishandi Village, Xiangyuan County, Changzhi, Shanxi Province Samples were stored at 4°C; 0-20cm soil samples were collected from the weeds (N 37°08′25.485″, E 111°30′65.453″) of the Ke’e Village dump in Xiaoyi, Shanxi, and stored at 4°C.

[0033] 2. Primary screening of strains

[0034] Weigh 10g of the reclaimed soil in the mining area and add it to 90mL of sterilized 250mL Erlenmeyer flask filled with glass beads and sterile water, shake it under the constant temperature shaker at 30°C and 180r / min for 20min, let it stand for 5min, and absorb 1m...

Embodiment 2

[0071] This embodiment illustrates the preparation of the cultivation of the spindle-shaped lysine bacillus of the present invention and the microbial inoculant

[0072] A spindle-shaped lysine bacillus microbial preparation, which is obtained by expanding the spindle-shaped lysine bacillus and then fully mixing the active ingredient and carrier with a mass ratio of 2:10 to 20, and storing at room temperature;

[0073] The carrier is a solid carrier or a liquid carrier, wherein the solid carrier is a mineral material, a plant material, a polymer compound or an organic material; the mineral material is one or more of clay, kaolin, montmorillonite, zeolite and fly ash several kinds; the plant material is one or more of corn flour, starch, bean flour and peanut flour; the polymer compound is one or two of polyvinyl alcohol and polyglycol; the organic material is One or two of peat, humic acid and organic fertilizer;

[0074] The active ingredient is one or more of living cells o...

Embodiment 3

[0080] This embodiment illustrates the effect of the spindle-shaped lysine bacillus agent of the present invention on improving the nutrient content of the reclaimed soil in the mining area

[0081] Rapeseed rape and spring wheat were planted by the pot method, and the planting time was (60 days, 100 days) respectively. The substrate soil used for potting comes from the reclaimed land of the coal mining subsidence area of ​​Lu'an Group located in the southeast of Shanxi Province, at the western foot of Taihang Mountain.

[0082] Rapeseed soil organic matter content 5.97g / kg, total nitrogen 0.50g / kg, total phosphorus 0.05g / kg, total potassium 20.00g / kg, alkaline nitrogen 42.82mg / kg, available phosphorus 3.39mg / kg, available potassium 98.60mg / kg kg, pH8.29.

[0083] Spring wheat soil organic matter content 6.40g / kg, total nitrogen 0.40g / kg, total phosphorus 0.4g / kg, total potassium 19.80g / kg, alkaline nitrogen 48.76mg / kg, available phosphorus 2.09mg / kg, available potassium 98.5...

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