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A fusiform lysine bacillus with ethanethiol and dimethyl disulfide degradation ability and its application

A kind of Bacillus lysine, dimethyl disulfide technology

Active Publication Date: 2020-09-29
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no relevant reports and patents on the simultaneous degradation of ethanethiol and dimethyl disulfide using Spinosa lysinus.

Method used

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  • A fusiform lysine bacillus with ethanethiol and dimethyl disulfide degradation ability and its application
  • A fusiform lysine bacillus with ethanethiol and dimethyl disulfide degradation ability and its application
  • A fusiform lysine bacillus with ethanethiol and dimethyl disulfide degradation ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A strain of Lysinibacillus fusiformis GDUTAN6 with the ability to degrade ethanethiol and dimethyl disulfide at the same time, the strain is preserved in the China Center for Type Culture Collection , the deposit address is Wuhan University Campus, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province; the deposit number is CCTCC NO: M 2018416, and the deposit date is July 2, 2018.

[0027] The spindle-shaped lysine bacillus GDUTAN6 of the present embodiment was isolated and screened from a landfill leachate in Guangzhou City, Guangdong Province, and its separation and purification method was as follows:

[0028] The acclimatization medium used is inorganic salt medium (every 1000mL inorganic salt medium contains K 2 HPO 4 ·3H 2 O1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, 1mL trace element solution (g / L): CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09g...

Embodiment 2

[0053] This example is the application of Lysinibacillus fusiformis GDUTAN6 in environmental restoration, which can degrade ethanethiol in the environment. The environment includes the atmosphere, water body or soil.

[0054] The method that the ethanethiol degradation ability of the spindle-shaped lysine bacillus (Lysinibacillus fusiformis) GDUTAN6 obtained by the screening of the present invention is verified as follows below:

[0055] According to the needs of the degradation experiment, the inorganic salt medium needs to be configured, and 45 mL of the inorganic salt solution is added to the 250 mL serum bottle (every 45 mL of the inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.054g, KH 2 PO 4 0.054g, NH 4 Cl 0.018g, MgSO 4 ·7H 2 O0.009g, FeSO 4 ·7H 2 O 0.00045g, CaCl 2 2H 2 O 0.009g, MnSO 4 4H 2 O 0.009g, CuSO 4 2H 2 O0.00045g, ZnSO 4 ·7H 2 O 0.009g, CoCl 2 ·6H 2 O 0.00405g, Na 2 MoO 4 2H 2 O 0.054g, H 3 BO 3 0.00027g, double distilled water ...

Embodiment 3

[0061] This example is the application of Lysinibacillus fusiformis GDUTAN6 in environmental remediation, which can also degrade dimethyl disulfide in the environment. The environment includes the atmosphere, water body or soil.

[0062] The method that the dimethyl disulfide degradation ability of the spindle-shaped lysine bacillus (Lysinibacillus fusiformis) GDUTAN6 obtained by the screening of the present invention is verified as follows below:

[0063] Configure inorganic salt medium according to the needs of the degradation experiment, add 45 mL of inorganic salt solution to the 250 mL serum bottle (every 45 mL of inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.054g, KH 2 PO 4 0.054g, NH 4 Cl 0.018g, MgSO 4 ·7H 2 O0.009g, FeSO 4 ·7H 2 O 0.00045g, CaCl 2 2H 2 O 0.009g, MnSO 4 4H 2 O 0.009g, CuSO 4 2H 2 O0.00045g, ZnSO 4 ·7H 2 O 0.009g, CoCl 2 ·6H 2 O 0.00405g, Na 2 MoO 4 2H 2 O 0.054g, H 3 BO 3 0.00027g, double distilled water 45mL), autoclave...

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Abstract

The invention discloses a spindle-shaped lysine bacillus strain capable of degrading ethanethiol and dimethyl disulfide and application thereof. The name of the strain is Lysinibacillus fusiformis GDUTAN6, and it is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan City, Hubei Province, the preservation number is CCTCC NO: M 2018416, and the preservation date is 2018 July 2nd. The spindle-shaped lysine bacillus GDUTAN6 is Gram-positive, rod-shaped, and the colony is round, white and transparent, and the diameter of the colony is 1-2 mm. The spindle-shaped lysine bacillus GDUTAN6 can be applied to environmental remediation, degrading ethanethiol and dimethyl disulfide in the environment, with a high degradation rate, and the degradation rates of ethanethiol and dimethyl disulfide can reach 93.17% respectively and 100%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and more specifically relates to a spindle-shaped lysine bacillus strain capable of degrading ethanethiol and dimethyl disulfide and application thereof. Background technique [0002] As we all know, organic odor pollution is a very serious air pollution that exists all over the world. It has caused great troubles to people's lives and seriously affected people's physical and mental health. Among them, sulfur-containing odor pollutants are a very important type of odor pollutants. Odor pollution is mainly a kind of olfactory pollution that is perceived by the human olfactory organs through the spread and diffusion of the atmosphere due to the volatility of this kind of odor pollutants. This type of pollutants usually includes volatile inorganic compounds (Volatile Inorganic Compounds, VIC) and volatile organic compounds (Volatile Organic Compounds, VOCs). Wherein ethanethiol (ethanethiol, CH ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C02F3/34B09C1/10C12R1/01C02F101/34
CPCB01D53/72B01D53/84B09C1/10C02F3/34C02F2101/34C02F2101/40C12N1/205C12R2001/01Y02A50/20
Inventor 李桂英王集军安太成熊举坤
Owner GUANGDONG UNIV OF TECH
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