Preparation method of coal gangue microbial fertilizer

The technology of a microbial fertilizer and a production method is applied in the production field of coal gangue microbial fertilizer, and can solve the problems of low carbon content, bulging, pulverization and the like, and achieve the effect of improving the phosphorus absorption coefficient, simple and reliable process, and improving ability.

Active Publication Date: 2020-11-10
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When coal gangue is used as fuel, the carbon content is too low, resulting in low efficiency; when used as building materials, due to the oxidation of sulfur and organic matter in coal gangue, it will ca...

Method used

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  • Preparation method of coal gangue microbial fertilizer
  • Preparation method of coal gangue microbial fertilizer
  • Preparation method of coal gangue microbial fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The separation of spindle-shaped lysine bacillus GZU-Lys01 comprises the following steps:

[0025] 1. Isolation and screening of functional strain GZU-Lys01

[0026] 1) Collection of samples

[0027] (1) The sample was collected from a coal gangue hill in a coal production area, and the fully weathered gangue sample and nearby soil samples were selected, and the collected samples were put into a collection bag;

[0028] (2) crush and sieve the sample collected, and collect a 100-mesh sample;

[0029] (3) Store the samples in a refrigerator at 4°C for later use.

[0030] 2) Screening of functional strains

[0031] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water;

[0032] (2) Shake in a constant temperature shaker at 180r / min, 27°C for 30min;

[0033] (3) After shaking for 30 minutes, take the supernatant and dilute to 1.0×10 -2 , 1.0×10 -3 , 1.0×10 -4 , 1.0×10 -5 , 1.0×10 -6 , 1.0×10 -7 .

[0034] (4) ...

Embodiment 2

[0046] Embodiment 2: GZU-Lys01 bacterial strain appearance morphology and Gram staining

[0047] The appearance of the colony of the strain on LB solid medium is milky white, opaque and round, with a smooth and moist surface, the colony is raised, shiny, with neat edges and sticky texture. Its size is about 1mm, see figure 2 .

[0048] By Gram staining, the morphology of the bacteria is as follows image 3 , the strain is a Gram-positive bacillus.

Embodiment 3

[0049] Embodiment 3: the cultivation of GZU-Lys01 bacterial strain

[0050] 1) Under sterile conditions, thaw the GZU-Lys01 strain at room temperature.

[0051] 2) After thawing, under aseptic conditions, shake well, absorb the GZU-Lys01 bacterial solution into the LB solid medium, spread it evenly, and place it in a constant temperature incubator at 35°C for 24-48h.

[0052] 3) Under sterile conditions, pick a single colony with an inoculation loop and inoculate it in LB solid medium by streaking method under sterile conditions, and place it in a constant temperature incubator at 35°C for 24-48 hours.

[0053]4) After the bacteria grow well, under aseptic conditions, use sterile water to elute the grown bacteria in the culture medium to obtain the bacteria liquid, and the concentration range of the bacteria liquid is controlled at: 1.05×10 10 -3.52×10 10 cfu / mL.

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Abstract

The invention discloses a preparation method of a coal gangue microbial fertilizer, wherein the coal gangue microbial fertilizer is prepared by dissociating coal gangue serving as a raw material by using lysinibacillus fusiformis GZU-Lys01. According to the method, waste coal gangue is recycled, and the method is simple in preparation process, mild in condition, green and environment-friendly.

Description

technical field [0001] The invention relates to a preparation method of microbial mineral fertilizer, in particular to a preparation method of coal gangue microbial fertilizer. Background technique [0002] Coal gangue is a kind of black-gray rock with low carbon content and harder than coal associated with coal seams. It is a solid waste generated in the process of coal mining and washing. It is one of the industrial wastes with the largest accumulation in my country at present. one. At present, the main disposal method of coal gangue is open-air accumulation, and a large amount of coal gangue stacking will cause a series of hazards: (1) a large amount of coal gangue stacking occupies land resources, and there are safety hazards such as dam failure; (2) weathering and spontaneous combustion of coal gangue will Generate dust, emit CO, CO 2 , SO 2 、H 2 S. NO X (3) After the coal gangue is washed by rainwater, its components will be gradually dissolved and flow into the so...

Claims

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Application Information

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IPC IPC(8): C05G3/00C12N1/20C12R1/01
CPCC05G3/00C12R2001/01C12N1/205Y02W30/40
Inventor 谢承卫高弦王应兰吉俐李小军董丽敏
Owner GUIZHOU UNIV
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