Application of lysinibacillus fusiformis with MICP function in greening restoration of tailings
A technology of Bacillus lysinus and Bacillus, applied in the restoration of contaminated soil, etc., can solve the lack of MICP strains for the exploration of seed enhancement technology, soil restoration sustainability and landscape restoration loopholes, lack of different particle sizes The standard of slag application rate and other issues can be achieved to improve the effect of phytoremediation, shorten the germination cycle of seeds, and achieve the effect of good curing
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Embodiment 1
[0038] Embodiment 1: determine in laboratory when fermenting 48-72h bacillus lysinica fermented liquid OD 600 The largest, about 2, its effect on Ca 2+ The curing effect is the best. The ratio of bacteria solution and reaction solution in the reaction system is 1:1, which has the best precipitation effect. At the same time, a solidification test was carried out in a greenhouse with natural light source (average maximum temperature T=34±0.23°C), and the slag with different particle sizes was determined: K1( ≤5mm), K2 (5~15mm), K3 (≥15mm) each particle size of slag fully solidified bacteria agent and reaction solution usage. The dosage of fully cured bacterial agent and reaction solution is as follows: pour 200ml / kg of cementing solution and bacterial solution into K1, pour about 185ml / kg of cementing solution and bacterial solution into K2, and pour about 165ml / kg into K3 The mixture of cementing liquid and bacterial liquid is poured in two times according to the penetration ...
Embodiment 2
[0039] Embodiment 2: get fermented liquid (OD 600 =1.8-2.0) dilute 5 times, 10 times, 20 times and 40 times of bacteria solution 50ml, get three repetitions of the seeds of the same cleanliness Escarinae chinensis (100 seeds for each repetition), soak in the spindle-type lysine of different dilution multiples Acidobacillus fermented liquid, after 24 hours, finish soaking and take out the treated seeds and blank control (distilled water soaking treatment) to sow in standard germination box, and nutrient soil and vermiculite (nutrient soil: vermiculite=2:1) are housed in the germination box, As for the artificial climate chamber to observe the germination progress. The result shows that the diluted lysine bacillus fermented liquid can obviously increase the germination rate of Prunus edulis and shorten the germination period, wherein the effect of diluting 40 times of bacterium liquid is better, and 20 times and 10 times of diluting bacterium liquid promote germination effect ...
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