Application of lysinibacillus fusiformis with MICP function in greening restoration of tailings

A technology of Bacillus lysinus and Bacillus, applied in the restoration of contaminated soil, etc., can solve the lack of MICP strains for the exploration of seed enhancement technology, soil restoration sustainability and landscape restoration loopholes, lack of different particle sizes The standard of slag application rate and other issues can be achieved to improve the effect of phytoremediation, shorten the germination cycle of seeds, and achieve the effect of good curing

Active Publication Date: 2021-06-18
NINGXIA UNIVERSITY +1
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AI Technical Summary

Problems solved by technology

[0005] (2) The MICP reaction system is optimized, and there is a lack of standards for the application amount of slag with different particle sizes
[0007] (3) The pure MICP tailings restoration technology leads to a single landscape pattern and low ecological benefits
[0008] Although the simple MICP solidification technology can fix the slag on the damaged mountain body and solidify free heavy metals, it has no practical significance for the restoration of regional vegetation. The solidified surface layer forms a hard shell, which is not conducive to the accumulation of soil moisture and nutrients in the northwest region where the ecological environment is f

Method used

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  • Application of lysinibacillus fusiformis with MICP function in greening restoration of tailings
  • Application of lysinibacillus fusiformis with MICP function in greening restoration of tailings
  • Application of lysinibacillus fusiformis with MICP function in greening restoration of tailings

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1: determine in laboratory when fermenting 48-72h bacillus lysinica fermented liquid OD 600 The largest, about 2, its effect on Ca 2+ The curing effect is the best. The ratio of bacteria solution and reaction solution in the reaction system is 1:1, which has the best precipitation effect. At the same time, a solidification test was carried out in a greenhouse with natural light source (average maximum temperature T=34±0.23°C), and the slag with different particle sizes was determined: K1( ≤5mm), K2 (5~15mm), K3 (≥15mm) each particle size of slag fully solidified bacteria agent and reaction solution usage. The dosage of fully cured bacterial agent and reaction solution is as follows: pour 200ml / kg of cementing solution and bacterial solution into K1, pour about 185ml / kg of cementing solution and bacterial solution into K2, and pour about 165ml / kg into K3 The mixture of cementing liquid and bacterial liquid is poured in two times according to the penetration ...

Embodiment 2

[0039] Embodiment 2: get fermented liquid (OD 600 =1.8-2.0) dilute 5 times, 10 times, 20 times and 40 times of bacteria solution 50ml, get three repetitions of the seeds of the same cleanliness Escarinae chinensis (100 seeds for each repetition), soak in the spindle-type lysine of different dilution multiples Acidobacillus fermented liquid, after 24 hours, finish soaking and take out the treated seeds and blank control (distilled water soaking treatment) to sow in standard germination box, and nutrient soil and vermiculite (nutrient soil: vermiculite=2:1) ​​are housed in the germination box, As for the artificial climate chamber to observe the germination progress. The result shows that the diluted lysine bacillus fermented liquid can obviously increase the germination rate of Prunus edulis and shorten the germination period, wherein the effect of diluting 40 times of bacterium liquid is better, and 20 times and 10 times of diluting bacterium liquid promote germination effect ...

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Abstract

The invention provides application of lysinibacillus fusiformis with an MICP function in greening restoration of tailings. The application comprises the following steps: taking quantitative slag around mine tailings in a mining area according to regional ecological environment characteristics, taking the slag back to a laboratory for liquid Yao culture, and screening microorganisms with an MICP function; according to a color developing agent result and a 16S analysis result, confirming that one strain is lysinibacillus fusiformis, and confirming the function of decomposing urea to induce calcium carbonate precipitation of the lysinibacillus fusiformis; determining the MICP function of the strain according to physicochemical properties of the strain, especially calcium carbonate precipitation reaction; on the basis of the MICP solidified alien soil regreening composite ecological restoration technology, adopting alien soil for planting plants to improve the ecological environment of the mining area, and realizing the tailing regreening effect. The application has a good tailing solidification effect, can solidify slag, has a promotion effect, namely a seed enhancement effect, on regional plant seed germination, and is beneficial to reduction of regional mine restoration technology cost.

Description

technical field [0001] The invention belongs to the technical field of treatment and recovery of destroyed mountain-heavy metal tailings, and in particular relates to an application technology of spindle-shaped lysine bacillus with MICP function in tailings greening restoration. Background technique [0002] The process of industrialization and rapid economic development has accelerated the destruction of natural resources and accompanied by environmental pollution. In the economically underdeveloped regions of the west, there is a lack of high-tech and new energy industries. In some places, the development model of breaking through the environmental carrying capacity to obtain economic benefits is popular. The indiscriminate mining and excessive digging of copper and silver mines have made the fragile mountains in the ecological transition zone riddled with holes, and the natural landscape, geological relics, tourism resources and ecological environment have been severely d...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/10B09C1/105B09C2101/00
Inventor 岳健敏宋乃平孟晨谢莉马昀潘响亮张道勇
Owner NINGXIA UNIVERSITY
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