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Inhibition method of microcystis aeruginosa

A technology of Microcystis aeruginosa and Houttuynia cordata, applied in the biological field

Active Publication Date: 2013-12-25
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the inhibition of Microcystis aeruginosa using the biological material Houttuynia cordata

Method used

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  • Inhibition method of microcystis aeruginosa
  • Inhibition method of microcystis aeruginosa
  • Inhibition method of microcystis aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this example, Microcystis aeruginosa PCC7806 (M.aeruginosat PCC7806) was provided by the algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences, and was cultivated on BG-11 medium. The main components of BG-11 medium were: NaNO3, 1.5g / L; K2HPO4 3H2O, 0.04g / L; MgSO4 7H2O, 0.075g / L; CaCl2 2H2O, 0.036g / L; citric acid, 0.006g / L; ferric ammonium citrate, 0.006g / L; EDTA, 0.001g / L ;Na2CO3, 0.02g / L; A5solution, 0.1mL; A5solution formula: H3BO3, 2.86g / L; MnCl2 4H2O, 1.86g / L; ZnSO4 7H2O, 0.22g / L; Na2MoO4 2H2O, 0.39g / L ; CuSO4.·5H2O, 0.08g / L; Co(NO3)2·6H2O, 0.05g / L.

[0022] Microcystis aeruginosa was expanded for culture. The culture conditions are: light intensity 4000lx, light-dark ratio 12h:12h, temperature (25±1)°C, shaking 3-5 times a day, so that the algae cells enter the logarithmic growth phase.

[0023] The inhibition rate (inhibition rate) was calculated according to the following formula:

[0024] IR=(1-N / N 0 )×100%

[0025] In the formula, IR...

Embodiment 2

[0030] The method is generally the same as in Example 1, except that when preparing the water extract of Houttuynia cordata, Houttuynia cordata powder and distilled water are leached for 6 days at a mass ratio of 1:15.

Embodiment 3

[0032] The method is generally the same as in Example 1, except that when preparing the water extract of Houttuynia cordata, Houttuynia cordata powder and distilled water are leached for 3 days at a mass ratio of 1:5.

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Abstract

The invention relates to an inhibition method of microcystis aeruginosa. A cordate houttuynia extract is used for inhibition. The inhibitor raw material of the inhibition method is rich and easy to get and environment-friendly during the treatment process of cyanobacterial bloom, and does not cause secondary pollution on water.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to a method for inhibiting Microcystis aeruginosa. Background technique [0002] Water bloom is one of the water eco-environmental problems that the society pays more attention to and needs to be solved urgently. Exploring effective prevention and control methods is of great significance to protect the water environment. Microcystis aeruginosa is a common bloom cyanobacteria that can produce microcystins. When it is enriched in fish or shellfish and passed to the human body through the food chain, it will pose a threat to human health and effectively prevent the occurrence of cyanobacteria blooms has important practical significance. [0003] There have been a large number of studies on algae inhibition at home and abroad, among which there are many reports on the use of allelochemicals isolated from plants to inhibit algae. For example, flavonoids extracted from orange peel ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01P1/00C02F1/50
Inventor 张庭廷刘璐李诚夏文彤杨晓辉
Owner ANHUI NORMAL UNIV
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