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Method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa

A real-time measurement technology of Microcystis aeruginosa, which can be used in color/spectral property measurement, fluorescence/phosphorescence, material excitation analysis, etc.

Inactive Publication Date: 2013-05-01
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0003] At present, most of the chlorophyll-a standard products used in the fluorescence method are purchased from foreign countries, and the price is expensive and the dosage is small, which is difficult to meet the analysis experiments of chlorophyll content in a large number of water bodies.

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  • Method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa
  • Method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa
  • Method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa

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Embodiment Construction

[0046] The following examples illustrate the invention in detail. Various raw materials and various equipments used in the present invention are conventional commercially available products, and can be directly obtained through market purchase. Among them, the test alga Microcystis aeruginosa can be purchased from Wuhan Institute of Hydrobiology, Chinese Academy of Sciences. Microcystis aeruginosa belongs to the cyanobacteria category, using BG-11 medium, and the formulation of the medium for algae species refers to the formulation provided by the Wuhan Institute of Hydrobiology, Chinese Academy of Sciences. The cultivation method includes: ① inoculation. Absolute aseptic operation is required in the whole process of algae cultivation, and cultivation is carried out under light and temperature conditions suitable for algae growth, and transfer once every 7-10 days. ② Domestication and simultaneous cultivation of algae species, transfer the preserved liquid algae species to fr...

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Abstract

The invention discloses a method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa. The method comprises the following steps: A, preparing the microcystis aeruginosa chlorophyll a standard solution; B, measuring the concentration of chlorophyll a in a stock solution, and calibrating the concentration by the national standard method; C, achieving fluorimetric determination of the stock solution and respectively measuring the fluorescence intensities of all solutions; D, building a microcystis aeruginosa standard fluorescence curve, and drawing a microcystis aeruginosa chlorophyll a fluorescence curve according to the concentration and the fluorescence intensity of the stock solution; and E, building a microcystis aeruginosa calibration fluorescence curve. According to the calibration curve between the fluorescence intensity of the microcystis aeruginosa solution and the concentration of the chlorophyll a, the concentration of the chlorophyll a can be real-timely and quickly obtained only by directly measuring the fluorescence intensity of water samples through a luminoscope.

Description

technical field [0001] The invention relates to the technical field of chlorophyll-a detection in water bodies, in particular to an inexpensive method capable of measuring chlorophyll-a content in water bodies in real time on-line. Background technique [0002] For the determination of chlorophyll a content in water, compared with traditional methods such as ultraviolet spectrophotometry and high performance liquid chromatography, which have the disadvantages of complicated steps, time-consuming and inconvenient online monitoring, direct fluorescence method is easier to operate and has higher sensitivity, and The results were not significantly different from those of traditional methods. The basic principle of measuring chlorophyll by fluorescence method is that when chlorophyll a is excited by light with a wavelength of about 450nm, it will emit fluorescence with a wavelength of about 680nm, and the intensity of fluorescence is related to the content of chlorophyll a. The ...

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Application Information

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IPC IPC(8): G01N21/33G01N21/64
Inventor 崔建升程玉欣王晓辉王改珍
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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