Algae inhibitor for verdigris microcystis aeruginosa

Abstract

The invention discloses an algae inhibitor for verdigris microcystis aeruginosa. The algae inhibitor is composed of n-nonanoic acid and linoleic acid in the volume ratio of 1:1, the applicable concentration range of the algae inhibitor in verdigris microcystis aeruginosa ranges from 0.04mL / L to 0.08mL / L. Compared with the prior art, two different kinds of fatty acids, namely the n-nonanoic acid and the linoleic acid, are selected to be combined, after inhibition effects of the combined fatty acids are evaluated by means of three kinds of different evaluation systems, the result indicates that the combination of the fatty acids has excellent synergic inhibition effects indeed. Further, the algae inhibitor is low in cost, environment-friendly and safe.

Description

technical field

[0001] The invention belongs to an algae inhibitor, in particular to an algae inhibitor for Microcystis aeruginosa. Background technique

[0002] At present, the use of allelochemicals to inhibit the growth of algae is the main research direction of algal bloom control. Because allelochemicals are secondary metabolites produced during the growth of aquatic plants, they can generally be degraded in the natural environment and will not accumulate in the ecosystem for a long time, so they have good ecological security. Allelochemicals can be divided into five categories according to their chemical structure, namely: aromatics, oxygen-containing heterocyclic compounds, terpenoids and nitrogen-containing compounds, and aliphatics. But up to now, it is more to utilize single allelochemical substance as algastatic agent, but the price of some allelochemical substance is relatively high, or has smell, and is all unfavorable for the use of algastatic agent. Content...

Examples

Embodiment 1

[0043] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50 mL Erlenmeyer flask, and then pour it into BG-11 medium (refer to the information of freshwater algae species bank for graduate students of aquatic biology in the Chinese Academy of Sciences) to make the algae cells start. The density is 7.0×10 5 per milliliter, the volume is 20 mL. Prepare different concentrations of algastatic agents (the volume ratio of nonanoic acid and linoleic acid is 1:1), nonanoic acid and linoleic acid, and measure their inhibition rate on Microcystis aeruginosa. The results are shown in Table 1, 2, 3 shows:

[0044] Table 1 Inhibition rate of combination of nonanoic acid and linoleic acid against Microcystis aeruginosa (%)

[0045]

[0046] The data in the table are mean±SE (n=3).

[0047] Table 2: Inhibition rate (%) of nonanoic acid to Microcystis aeruginosa

[0048]

[0049] The data in the table are mean±SE (n=3).

[0050] Table 3 Inhibiti...

Embodiment 2

[0059] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50 mL Erlenmeyer flask, and then pour into the medium to make the initial density of algal cells 7.0×10 5 per milliliter, the volume is 20 mL. Prepare different concentrations of algastatic agents (the volume ratio of nonanoic acid and linoleic acid is 1:1) to measure their effects on the protein content of Microcystis aeruginosa algae liquid, and the results are shown in Table 5: Table 5 Nonanoic acid and Effects of linoleic acid combined on the protein content of Microcystis aeruginosa

[0060]

[0061] The data in the table are mean ± SE (n=3),

[0062] * Compared with the control group in the same period, P** Compared with the control group in the same period, P<0.01

[0063] It can be seen from Table 5 that under the combined action of pelargonic acid and linoleic acid, the protein content of the algae liquid increased with the increase of the concentration, 1.0×10 -2 mL...

Embodiment 3

[0065] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50 mL Erlenmeyer flask, and then pour into the medium to make the initial density of algal cells 7.0×10 5 per milliliter, the volume is 20 mL. Different concentrations of algastatic agents were prepared (the volume ratio of nonanoic acid and linoleic acid was 1:1) to determine their effects on the nucleic acid content of Microcystis aeruginosa liquid. The results are shown in Table 6: Table 6 Nonanoic acid and Effects of Linoleic Acid on the Nucleic Acid Content of Microcystis aeruginosa Liquid

[0066]

[0067] The data in the table are mean±SE (n=3).

[0068] * Compared with the control group in the same period, P** Compared with the control group in the same period, P<0.01

[0069] Table 6 shows the changes in the nucleic acid content of Microcystis aeruginosa liquid. It can be seen from the table that the nucleic acid content of algae liquid increased gradually with the in...

technical field

[0001] The invention belongs to an algae inhibitor, in particular to an algae inhibitor for Microcystis aeruginosa. Background technique

[0002] At present, the use of allelochemicals to inhibit the growth of algae is the main research direction of algal bloom control. Because allelochemicals are secondary metabolites produced during the growth of aquatic plants, they can generally be degraded in the natural environment and will not accumulate in the ecosystem for a long time, so they have good ecological security. Allelochemicals can be divided into five categories according to their chemical structure, namely: aromatics, oxygen-containing heterocyclic compounds, terpenoids and nitrogen-containing compounds, and aliphatics. But up to now, it is more to utilize single allelochemical substance as algastatic agent, but the price of some allelochemical substance is relatively high, or has smell, and is all unfavorable for the use of algastatic agent. Content...

Examples

Embodiment 1

[0043] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50 mL Erlenmeyer flask, and then pour it into BG-11 medium (refer to the information of freshwater algae species bank for graduate students of aquatic biology in the Chinese Academy of Sciences) to make the algae cells start. The density is 7.0×10 5 per milliliter, the volume is 20 mL. Prepare different concentrations of algastatic agents (the volume ratio of nonanoic acid and linoleic acid is 1:1), nonanoic acid and linoleic acid, and measure their inhibition rate on Microcystis aeruginosa. The results are shown in Table 1, 2, 3 shows:

[0044] Table 1 Inhibition rate of combination of nonanoic acid and linoleic acid against Microcystis aeruginosa (%)

[0045]

[0046] The data in the table are mean±SE (n=3).

[0047] Table 2: Inhibition rate (%) of nonanoic acid to Microcystis aeruginosa

[0048]

[0049] The data in the table are mean±SE (n=3).

[0050] Table 3 Inhibiti...

Embodiment 2

[0059] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50 mL Erlenmeyer flask, and then pour into the medium to make the initial density of algal cells 7.0×10 5 per milliliter, the volume is 20 mL. Prepare different concentrations of algastatic agents (the volume ratio of nonanoic acid and linoleic acid is 1:1) to measure their effects on the protein content of Microcystis aeruginosa algae liquid, and the results are shown in Table 5: Table 5 Nonanoic acid and Effects of linoleic acid combined on the protein content of Microcystis aeruginosa

[0060]

[0061] The data in the table are mean ± SE (n=3),

[0062] * Compared with the control group in the same period, P** Compared with the control group in the same period, P<0.01

[0063] It can be seen from Table 5 that under the combined action of pelargonic acid and linoleic acid, the protein content of the algae liquid increased with the increase of the concentration, 1.0×10 -2 mL...

Embodiment 3

[0065] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50 mL Erlenmeyer flask, and then pour into the medium to make the initial density of algal cells 7.0×10 5 per milliliter, the volume is 20 mL. Different concentrations of algastatic agents were prepared (the volume ratio of nonanoic acid and linoleic acid was 1:1) to determine their effects on the nucleic acid content of Microcystis aeruginosa liquid. The results are shown in Table 6: Table 6 Nonanoic acid and Effects of Linoleic Acid on the Nucleic Acid Content of Microcystis aeruginosa Liquid

[0066]

[0067] The data in the table are mean±SE (n=3).

[0068] * Compared with the control group in the same period, P** Compared with the control group in the same period, P<0.01

[0069] Table 6 shows the changes in the nucleic acid content of Microcystis aeruginosa liquid. It can be seen from the table that the nucleic acid content of algae liquid increased gradually with the in...

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