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Banana fusarium oxysporum culture medium and application thereof

A technology of banana fusarium wilt bacteria and culture medium, applied to fungi, methods using spores, methods based on microorganisms, etc., can solve the problems of interference with Foc and low germination rate of Foc conidia, achieve simple preparation methods and promote germination , the effect suitable for promotion and application

Active Publication Date: 2018-01-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these existing mediums of Fusarium wilt of banana, there are many factors that interfere with the study of Foc secreted proteins: Among them, some components in PDA medium and CM medium (potato, peptone and yeast extract, etc.) contain complex Protein components; medium containing inorganic salts (Chaba’s medium and Bilai’s medium), there is a problem of low germination rate of Foc conidia, these factors are important factors that interfere with the analysis of Foc secreted proteins
In the prior art, there are no literature and patent reports that are suitable for the germination of conidia of Fusarium wilt of banana and are also conducive to the analysis of its secreted protein.

Method used

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  • Banana fusarium oxysporum culture medium and application thereof
  • Banana fusarium oxysporum culture medium and application thereof
  • Banana fusarium oxysporum culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A culture medium for Fusarium wilt of banana (coded as NCM medium), containing the following components: the final concentration of glucose is 10g / L, the final concentration of aspartic acid is 4 g / L, 20×nitrate, 200×iron The volume fractions of salt, 1000×vitamins, and 1000×trace elements are 5%, 0.5%, 0.1%, 0.1% respectively, and the balance of deionized water; the pH value of the culture medium is 6.5.

[0028] The composition of the 20× nitrate is: NaNO 3 120 g, KCl 10.4 g, MgSO 4 ·7H 2 O 10.4 g, KH 2 PO 4 30.4 g, add distilled water to make up to 1 L.

[0029] The composition of the 200× iron salt is: FeSO 4 ·7H 2 O 7.5 g, Na 2 EDTA·2H 2 O 5.6 g, add distilled water to 1 L.

[0030] The composition of the 1000×vitamin is: glycine 2 g, niacin 0.5 g, biotin 0.5 g, pyridoxine hydrochloride 0.5 g, thiamine hydrochloride 0.4 g, riboflavin 0.5 g, inositol 0.1 g, plus deionized water to 1 L.

[0031] The composition of the 1000×trace elements is: ZnSO 4 ·7H 2...

Embodiment 2

[0034] A culture medium for Fusarium wilt of banana (coded as NCM-2 medium), containing the following components: the final concentration of glucose is 10g / L, the final concentration of aspartic acid is 2 g / L, 20×nitrate, 200 The volume fractions of ×iron salt, 1000×vitamins, and 1000×trace elements are 5%, 0.5%, 0.1%, 0.1% respectively, and the balance of deionized water; the pH value of the medium is 6.5.

[0035] The composition of the 20× nitrate is: NaNO 3 120 g, KCl 10.4 g, MgSO 4 ·7H 2 O 10.4 g, KH 2 PO 4 30.4 g, add distilled water to make up to 1 L.

[0036] The composition of the 200× iron salt is: FeSO 4 ·7H 2 O 7.5 g, Na 2 EDTA·2H 2 O 5.6 g, add distilled water to 1 L.

[0037] The composition of the 1000×vitamin is: glycine 2 g, niacin 0.5 g, biotin 0.5 g, pyridoxine hydrochloride 0.5 g, thiamine hydrochloride 0.4 g, riboflavin 0.5 g, inositol 0.1 g, plus deionized water to 1 L.

[0038] The composition of the 1000×trace elements is: ZnSO 4 ·7H 2 O ...

Embodiment 3

[0041] A culture medium for Fusarium wilt of banana (the code is NCM-3 medium), which contains the following components: the final concentration of glucose is 10 g / L, the final concentration of aspartic acid is 8 g / L, 20 × nitrate, 200 The volume fractions of ×iron salt, 1000×vitamins, and 1000×trace elements are 5%, 0.5%, 0.1%, 0.1% respectively, and the balance of deionized water; the pH value of the medium is 6.5.

[0042] The composition of the 20× nitrate is: NaNO 3 120 g, KCl 10.4 g, MgSO 4 ·7H 2 O 10.4 g, KH 2 PO 4 30.4 g, add distilled water to make up to 1 L.

[0043] The composition of the 200× iron salt is: FeSO 4 ·7H 2 O 7.5 g, Na 2 EDTA·2H 2 O 5.6 g, add distilled water to 1 L.

[0044] The composition of the 1000×vitamin is: glycine 2 g, niacin 0.5 g, biotin 0.5 g, pyridoxine hydrochloride 0.5 g, thiamine hydrochloride 0.4 g, riboflavin 0.5 g, inositol 0.1 g, plus deionized water to 1 L.

[0045] The composition of the 1000×trace elements is: ZnSO 4...

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Abstract

The invention discloses a banana fusarium oxysporum culture medium and application thereof. The culture medium comprises the following components: 10g / L glucose, 2-8g / L aspartic acid, 5% by volume of20*nitrate, 0.5% by volume of 200*ferric salt, 0.1% by volume of 1000*vitamins and 0.1% by volume of 1000*trace elements, and the pH value of the culture medium is 6.2-6.5. By adopting the culture medium disclosed by the invention, germination of banana fusarium oxysporum conidium is promoted, interference of macromolecule proteins is avoided, and analysis on banana fusarium oxysporum secretion proteins is facilitated.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a culture medium for Fusarium wilt of banana and its application. Background technique [0002] Banana wilt is caused by Fusarium oxysporum Cuban specification ( Fusarium oxysporum f. sp. cubes , Foc) is a fungal disease caused by banana production, which is one of the most important diseases in banana production. It occurs in almost all banana production areas in my country. The incidence of diseased banana orchards is generally 30-50%. Reaching more than 90%, it is a serious threat to the sustainable development of the banana industry. Foc mainly invades from the roots or wounds of bananas through conidia, and then enters the vascular bundles for growth and reproduction, which leads to browning and necrosis of banana vascular bundle cells, and finally leads to the wilting of the whole banana plant. Due to the complex genetic background of Foc, its pathogeni...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12P21/00C12R1/77
Inventor 聂燕芳李云锋李华平周玲菀陈敏王振中
Owner SOUTH CHINA AGRI UNIV
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