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Control method for efficient production of calcium gluconate by microbial fermentation

A microbial fermentation method, calcium gluconate technology, applied in the field of bioengineering, can solve problems such as uncontrolled, and achieve the effect of precise control

Inactive Publication Date: 2013-10-16
山东福洋生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the fermentation of gluconic acid and its salts (such as sodium gluconate, calcium gluconate, etc.) methods such as residual sugar concentration, however, none of these methods can apply the oxygen transfer coefficient (Kla) to realize the online control of the fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The first step, seed cultivation: Add 2.5kg of glucose, 60g of corn steep liquor, 5.2g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L cultivation tank. To 38 ℃, add 250ml eggplant running bacteria suspension, control the dissolved oxygen (DO) ≥ 30, cultivate for 15h to get the seed solution.

[0016] The second step, fermentation: Add 4.0kg of glucose, 2.8g of magnesium sulfate, 1.4g of urea, and 2ml of defoamer to a 50L fermenter, set the volume to 13L, sterilize at 115°C for 15 minutes, then lower it to 38°C, and use 20% The calcium oxide suspension was adjusted to control pH5.4, and fermentation was started.

[0017] The third step, feeding:

[0018] (1) Ingredients: Add 14kg of 500g / L glucose solution, 20g of corn steep liquor, 5.6g of magnesium sulfate, 2.8g of urea, and 1ml of defoamer to a 30L feeding tank. To 38 ℃, aseptic transfer to serum bottles for later use.

[0019] ⑵ Feeding: In the second step, after 12 hours of...

Embodiment 2

[0027] The first step, seed cultivation: Add 2.5kg of glucose, 60g of corn steep liquor, 5.2g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L cultivation tank. To 38°C, add 250ml eggplant bacteria suspension, control the dissolved oxygen (DO) ≥ 30, and cultivate the seed solution for 16h.

[0028] The second step, fermentation: Add 4.0kg of glucose, 2.8g of magnesium sulfate, 1.4g of urea, and 2ml of defoamer to a 50L fermenter, set the volume to 13L, sterilize at 115°C for 15 minutes, then lower it to 38°C, and use 20% The calcium oxide suspension was adjusted to control pH5.4, and fermentation was started.

[0029] The third step, feeding:

[0030] (1) Ingredients: Add 14kg of glucose solution, 20g of corn steep liquor, 5.6g of magnesium sulfate, 2.8g of urea, and 1ml of defoamer to a 30L feeding tank, and set the volume to 20L, sterilize at 115°C for 30 minutes, and then lower it to 38°C , aseptically transferred to a serum bottle fo...

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Abstract

The invention discloses a control method for efficient production of calcium gluconate by microbial fermentation. The method includes: taking a gluconic acid high-producing strain as the starting strain, conducting seed culture, then transferring the strain into a fermentation tank to perform fermentation production of calcium gluconate, and during fermentation, according to an online measured oxygen transfer coefficient (Kla), automatically controlling the adding speed and adding amount of 500g / L glucose and nutrients. The method realizes direct, real-time and accurate control of a calcium gluconate fermentation process.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a control method for efficient production of calcium gluconate by microbial fermentation. Background technique [0002] Gluconic acid is widely used in food, medicine, chemical industry, water treatment, construction and other industries as a bulking agent, coagulant, chelating agent, and sour agent; gluconate (such as with sodium, calcium, zinc, ferrous and other metal oxides) Synthetic gluconate) can be used as food additives and nutritional supplements. [0003] The production of gluconic acid mainly includes microbial fermentation, electrolysis and catalytic oxidation. Due to the mild conditions and obvious energy saving of the microbial method, it is widely used at present. Among them, microbial fermentation includes fungal fermentation and bacterial fermentation process. Since Boutroux first used the oxidation of microorganisms to oxidize glucose into gluconic acid, the ferm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q3/00
Inventor 赵伟张雷达王健曹大鹏宋金松杨健
Owner 山东福洋生物科技股份有限公司
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