Quantitative fructose assay kit and application thereof as well as quantitative seminal plasma fructose assay method

Abstract

The invention discloses a quantitative fructose assay kit which comprises inorganic acid deproteinized extract A, inorganic base deproteinized extract B, fructose calibration solution, a reagent 1 containing 0.001-0.1mol / L adenosine triphosphate sodium salt, a reagent 2 containing 1-100KU / L hexokinase and 1-100KU / L glucose-6-phosphate dehydrogenase, and a reagent 3 containing 0.001-0.1mol / L nicotinamide adenine dinucleotide. The seminal plasma fructose assay method comprises the following steps: respectively adding the reagent 1 and the reagent 2 to deproteinized seminal plasma and the fructose calibration solution, and mixing uniformly; reacting at the temperature of 10-40 DEG C for 5-120 minutes, then reading the absorbance respectively at the wavelength of 280-400nm; adding the reagent 3 respectively, and mixing uniformly; reacting under the same conditions and reading the absorbance; and calculating the difference between the absorbance read at the first time and the absorbance read at the second time, and comparing or calculating the absorbance of a seminal plasma specimen and the fructose calibration solution to obtain the concentration of the seminal plasma fructose. The kit and the method can be used for quantitative determination of fructose in sera, plasma, body fluid, food and solid extracting solution, the methodology is special, unique, clean and environment-friendly, manual operation and automatic batch assay can be realized, and the kit and the method are easy to popularize and apply clinically.

Description

technical field [0001] The invention relates to a fructose quantitative detection kit and its application, and to a method for using the kit to detect the concentration of seminal plasma fructose in human seminal plasma specimens in vitro. Background technique [0002] Male bilateral ejaculatory duct obstruction, congenital absence of seminal vesicles or dysplasia, fructose was negative. The fructose content decreases in cases of vesiculitis, incomplete ejaculation, or excessive ejaculation. Fructose combined with other detection indicators (such as seminal plasma neutral α-glucosidase) can locate the obstruction site in patients with obstructive azoospermia. Seminal berry fructose is an evaluation function index of seminal vesicle secretion function. The level of testosterone affects the secretion of fructose in the seminal vesicles, and insufficient androgen can cause a decrease in fructose content. Decreased seminal vesicle secretion can lead to decreased semen volume....

AI Technical Summary

Problems solved by technology

The indole method is the methodology recommended by the World Health Organization, but it needs to use concentrated hydrochloric acid during the operation, which will cause harm to the operator and the environment; the resorcinol method has low sensitivity and needs to use concentrated hydrochloric acid, which will cause harm to the operator and the environment. Hazardous, and incubating at 90°C for color development, inconvenient for clinical use

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