Pseudomonas fluorescens strain, microbial inoculum and use thereof as seedling culture medium for controlling tomato bacterial wilt

A technology for Pseudomonas fluorescens, tomato bacterial wilt disease, applied in application, bacteria, biocide and other directions, can solve the problems of long crop rotation, difficult implementation, large outbreaks, etc., to achieve low cost, avoid secondary pollution, vitality strong effect

Inactive Publication Date: 2010-10-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paddy and dry rotation requires a long rotation period and no other hosts nearby to achieve good results, so it is difficult to implement
After the soil is sterilized, a soil biological vacuum is formed. Once the soil is infected by pathogenic bacteria, it will lead to a large outbreak of pathogenic bacteria and cause more seri

Method used

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  • Pseudomonas fluorescens strain, microbial inoculum and use thereof as seedling culture medium for controlling tomato bacterial wilt
  • Pseudomonas fluorescens strain, microbial inoculum and use thereof as seedling culture medium for controlling tomato bacterial wilt
  • Pseudomonas fluorescens strain, microbial inoculum and use thereof as seedling culture medium for controlling tomato bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1: Obtaining of Pseudomonas fluorescens ②-16' strain

[0014] The present invention provides a strain for controlling tomato bacterial wilt by using pathogenic bacteria antagonistic microorganisms, specifically Pseudomonas fluorescens (Pseudomonas fluorescens) ②-16'. In 2008, experimenters collected soil samples from different regions such as Jinhua, Taizhou, and Lishui, and used bacterial culture medium to isolate more than 300 strains of various bacteria from them by dilution separation method, and then purified the strains. Through the slant and shake flask culture at 28-30℃ and the inoculation effect in a variety of seedling substrates, the strain ②-16' that antagonizes tomato bacterial wilt is screened out.

[0015] 1 medium

[0016] Beef extract peptone agar medium: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, H 2 O 1000ml, pH 7.0-7.2. Sterilize at 121.3°C for 20 minutes.

[0017] 2 test steps

[0018] 2.1 Preparation of medium

[0019] Prepare beef extract ...

Example Embodiment

[0034] Example 2: Plate antagonism test of the isolated bacteria against Ralstonia solanacearum

[0035] 1. Medium

[0036] 1.1 Solid medium

[0037] Ralstonia solanacearum culture medium preparation: MgSO 4 7H 2 O 0.3g, K 2 HPO 4 2.0 g, Yeast extract 4.0 g, Casein hydrolysate 8.0 g, Sucrose 10.0 g, Agar 18 g, and Distilled water 1000 ml.

[0038] Preparation of beef extract peptone agar medium: same as in Example 1.

[0039] 1.2 Liquid medium

[0040] Bacterial liquid shake flask culture medium: Beef extract peptone culture medium does not add agar.

[0041] Tomato Ralstonia solanacearum liquid shake flask culture medium: Ralstonia solanacearum culture medium does not add agar.

[0042] 2. Test procedure

[0043] 2.1 Medium preparation

[0044] Preparation of Ralstonia solanacearum culture medium (solid, liquid), preparation of pathogenic bacterium Ralstonia solanacearum slant; preparation of beef extract peptone agar medium (solid, liquid), preparation of bacterial slant.

[0045] 2.2 Pla...

Example Embodiment

[0084] Example 3: Preparation of antagonist of strain ②-16'

[0085] The microbial agent capable of antagonizing tomato bacterial wilt of the present invention is cultivated and multiplied by Pseudomonas fluorescens (Pseudomonas fluorescens) ②-16' strain.

[0086] The specific operation process is as follows: Pseudomonas fluorescens ②-16' is based on beef extract medium, adding a small amount of inorganic salts such as calcium, magnesium, phosphorus, etc. to carry out liquid shake flask fermentation, culture at 25-35°C, rotating speed 120-220rpm. Use this as a strain to inoculate a liquid fermentor to make a liquid mother seed. The raw materials of the liquid fermentor are bran, vegetable cake, and bean cake powder (ratio 1%~5%:0~3%:0~3%:0~3%) ) And a small amount of inorganic salts, etc., the amount of water added is 99-86%. After autoclaving, when the temperature of the fermenter liquid is cooled to below 35℃, then 1.0-10% of the inoculum will be inoculated into the liquid fermen...

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Abstract

The invention provides a strain namely a pseudomonas fluorescens strain (2)-16' for controlling tomato bacterial wilt by using pathogenic bacteria antagonistic microbes, wherein the preservation serial number of the strain is CGMCC NO.3330. The invention also provides a microbial inoculum prepared from the pseudomonas fluorescens train (2)-16'. The invention further provides use of the pseudomonas fluorescens strain (2)-16' for controlling the tomato bacterial wilt, particularly as a seedling culture medium for controlling the tomato bacterial wilt. The pseudomonas fluorescens strain (2)-16' is directionally separated and selectively cultured, is cultured for propagation first, and then is prepared into a product of the microbial inoculum; mushroom residues after culturing edible fungi are fermented and thoroughly decomposed and then are used for preparing the seedling culture medium together with other base materials in certain proportion; and a raw anti-microbial agent is organically combined with the seedling culture medium directly. The pseudomonas fluorescens strain (2)-16' has a large number of advantages of abundant available resources, low cost and capacity of avoiding secondary pollutions, and provides an effective way for solving a soil-borne disease namely the tomato bacterial wilt.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a tomato bacterial wilt antagonistic strain and the field of biological control of tomato bacterial wilt by a seedling-raising substrate prepared by using the bacterial agent of the bacterial strain. Background technique [0002] Mushroom dregs are mainly discarded mushroom tubes after cultivation such as shiitake mushrooms and enoki mushrooms. The main components are wood dregs and cottonseed shells, which are mainly lignin and have low nutrient content. After being decomposed by edible fungi, the mushroom dregs are slightly acidic and soft , Its water absorption, water retention and fertility, and air permeability are good, and it is more suitable for the growth of seedling roots of flower seedlings. Therefore, mushroom dregs are more suitable for developing container seedling production substrates for flower seedlings. Therefore, the research and development g...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00C05G3/00C12R1/39
Inventor 王卫平薛智勇朱凤香陈晓旸洪春来吴传珍
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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