Pseudomonas fluorescens strain, microbial inoculum and use thereof as seedling culture medium for controlling tomato bacterial wilt

Abstract

The invention provides a strain namely a pseudomonas fluorescens strain (2)-16' for controlling tomato bacterial wilt by using pathogenic bacteria antagonistic microbes, wherein the preservation serial number of the strain is CGMCC NO.3330. The invention also provides a microbial inoculum prepared from the pseudomonas fluorescens train (2)-16'. The invention further provides use of the pseudomonas fluorescens strain (2)-16' for controlling the tomato bacterial wilt, particularly as a seedling culture medium for controlling the tomato bacterial wilt. The pseudomonas fluorescens strain (2)-16' is directionally separated and selectively cultured, is cultured for propagation first, and then is prepared into a product of the microbial inoculum; mushroom residues after culturing edible fungi are fermented and thoroughly decomposed and then are used for preparing the seedling culture medium together with other base materials in certain proportion; and a raw anti-microbial agent is organically combined with the seedling culture medium directly. The pseudomonas fluorescens strain (2)-16' has a large number of advantages of abundant available resources, low cost and capacity of avoiding secondary pollutions, and provides an effective way for solving a soil-borne disease namely the tomato bacterial wilt.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a tomato bacterial wilt antagonistic strain and the field of biological control of tomato bacterial wilt by a seedling-raising substrate prepared by using the bacterial agent of the bacterial strain. Background technique [0002] Mushroom dregs are mainly discarded mushroom tubes after cultivation such as shiitake mushrooms and enoki mushrooms. The main components are wood dregs and cottonseed shells, which are mainly lignin and have low nutrient content. After being decomposed by edible fungi, the mushroom dregs are slightly acidic and soft , Its water absorption, water retention and fertility, and air permeability are good, and it is more suitable for the growth of seedling roots of flower seedlings. Therefore, mushroom dregs are more suitable for developing container seedling production substrates for flower seedlings. Therefore, the research and development g...

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Problems solved by technology

The paddy and dry rotation requires a long rotation period and no other hosts nearby to achieve good results, so it is difficult to implement

After the soil is sterilized, a soil biological vacuum is formed. Once the soil is infected by pathogenic bacteria, it will lead to a large outbreak of pathogenic bacteria and cause more serious consequences, and it is not feasible to operate in the field

Cultivating grafted seedlings with disease-resistant wood has obvious effects on the prevention and control of soil-borne diseases, but it can only be achieved with good wood and suitable grafting techniques, and large-scale applications also have certain limitations.

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