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Manganese-oxidized pseudomonas T34, method for preparing biogenic manganese oxide and application of pseudomonas or biogenic manganese oxide in degrading ciprofloxacin

A technology of biological manganese oxide and pseudomonas, applied in the field of environmental remediation, can solve the problems of reducing the total reaction rate and electron transfer rate, and achieve the effect of rich performance, large-scale industrial promotion prospect, and simple and easy method

Active Publication Date: 2016-02-17
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, the introduction of electron-withdrawing substituents in the molecule will reduce the electron transfer rate, thereby reducing the overall reaction rate.

Method used

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  • Manganese-oxidized pseudomonas T34, method for preparing biogenic manganese oxide and application of pseudomonas or biogenic manganese oxide in degrading ciprofloxacin
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  • Manganese-oxidized pseudomonas T34, method for preparing biogenic manganese oxide and application of pseudomonas or biogenic manganese oxide in degrading ciprofloxacin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A manganese oxidizing bacteria strain Pseudomonassp.T34 is obtained by screening through the following steps:

[0048] (1). Weigh 1g of ferromanganese ore (Zaoyang, Hunan) sample, add it to a sterile water triangular flask containing 9mL and glass beads, reciprocate and shake at 200r / min, 30°C for 30min, shake to disperse bacteria;

[0049] (2). Make 10 -1 Bacterial suspension of dilution degree, after standing for 10min, take the supernatant and gradiently dilute to obtain the following three dilution degrees: 10 -4 、10 -5 、10 -6 , get three dilutions of 0.1mL dilutions and coat beef extract peptone medium (see Table 1), and each dilution is coated with three flat plates respectively;

[0050] (3). Plates were placed upside down in a 30°C incubator, observed and counted after 24-48 hours of incubation. At the same time, the dominant growth bacteria were picked, separated and purified by streaking, and the basic morphology of the isolated bacteria was observed unde...

Embodiment 2

[0058] Analysis of 16S rRNA in Pseudomonas sp.T34

[0059] The total DNA of the bacteria was extracted, and the 16SrRNA fragment was amplified by using the universal primer 1492r / F27 as a PCR primer. The resulting gene fragment was sequenced, and the sequence (shown in SEQ ID NO.1) was compared with the database, and it was found that the sequence was highly similar to the 16SrRNA sequence of Pseudomonas, thus presuming that the manganese oxidizing strain was Pseudomonas The genus Pseudomonassp. And named it as Pseudomonas sp.T34.

Embodiment 3

[0061] The cultivation of Pseudomonas sp.T34 and the preparation method of manganese oxide, the process is as follows:

[0062] (1) Pick the activated Pseudomonas T34 from the slant and inoculate it in the Mn-containing 2+ (final concentration is 1mmol / L) in the Lept culture medium (5mL), at 30 DEG C shaker, 150r / min cultivates 24h to prepare the bacterial suspension used for inoculation;

[0063] (2) Inoculate the above-mentioned bacterial suspension into the Mn-containing 2+ (Final concentration is 1mmol / L) Lept culture medium (recipe is shown in Table 2) (200ml), in 30 ℃, 150r / min shaker culture 2 days, promptly produce a large amount of manganese oxides;

[0064] (3) Collect the manganese oxide bacteria, 5000 rpm, 5min, pour the supernatant, keep the precipitate, wash the precipitate three times with deionized water, suspend with deionized water, store at 4°C for later use Example.

[0065] Formulation of table 2 Lept medium (each component content in 1L)

[0066]

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Abstract

The invention discloses manganese-oxidized pseudomonas T34, a method for preparing biogenic manganese oxide and application of the pseudomonas or the biogenic manganese oxide in degrading ciprofloxacin, and discloses a pseudomonas strain pseudomonassp. T34 which is capable of generating a manganese oxide, wherein the strain is preserved with preservation number of CCTCC NO: M2014168. The strain, in an environment with presence of low-valence manganese ions, can be used for oxidizing the low-valence manganese ions in the environment, so that a manganese dioxide compound is formed and the formed manganese dioxide compound is coated on the surfaces of bacteria. The biogenic manganese oxide generated from the strain, which is quite strong in catalytic oxidation capacity, is capable of degrading organic macro-molecular substances into micro-substances which can be absorbed and utilized by the bacteria with efficiency much higher than that of chemically synthesized manganese oxide. The manganese oxide generated from the strain has the advantages of being strong in catalysis capacity, environment-friendly, easy for production and the like, so that a new way is created for solving the current antibiotic pollution status in the environment.

Description

technical field [0001] The present invention relates to the field of applied microorganism technology and the field of environmental restoration, in particular to a strain of Pseudomonas T34 capable of producing manganese oxide, and to the preparation method of the bacterium-manganese oxide and the application of the bacterium in degrading ciprofloxacin . Background technique: [0002] Manganese oxides have an important impact on the migration and transformation of pollutants and nutrients in the environment through adsorption, catalysis and oxidation. Therefore, it plays a very important role in the biogeochemical cycle of heavy metal elements and organic carbon. Manganese oxide has adsorption ability and strong oxidation ability for various metal ions, and can also be used as a temporary electron acceptor in the process of cellular respiration in an anaerobic environment to oxidize H2 electron acceptor. Most of the manganese oxidation in the environment is of microbial o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00A62D3/02C02F3/34C12R1/38A62D101/28
Inventor 李林谭璐明王智勇张震
Owner HUAZHONG AGRI UNIV
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