Enzyme and microbial inoculum for decomposing lignocellulose

A technology for lignocellulose and Clostridium xylanolyticum, which is applied in the directions of enzymes, enzymes, bacteria, etc., can solve the problems of difficult decomposition of natural lignocellulose, failure to meet production requirements, low degradation efficiency of lignocellulose, etc. Simple preparation and high degradation rate

Inactive Publication Date: 2009-10-21
CHINA AGRI UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, under artificial culture conditions, it is difficult to directly decompose natural lignocellulose by relying on purely cultured microorganisms and enzymes.
At present, the degradation ef...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the composition of the compound bacterial strain of decomposing lignocellulose

[0025] Bacterial composition of complex bacterial strains decomposing lignocellulose:

[0026] Alcaligenes faecalis (purchased from the German Culture Collection of Microorganisms, the strain preservation number is DSM 16503);

[0027] Bacteroides xylanisolvens (Bacteroides xylanisolvens) (purchased from the German Culture Collection of Microorganisms, the strain preservation number is DSM 18836);

[0028] Clostridium xylanolyticum (Clostridium xylanolyticum) (purchased from the German Culture Collection of Microorganisms, the strain preservation number is DSM 6555T);

[0029] Clostridium lentocellum (Clostridium lentocellum) (purchased from the American Culture Collection, the strain preservation number is ATCC 49066);

[0030] Flavobacterium aquatile (Flavobacterium aquatile) (purchased from the American Culture Collection, the culture collection number is ATCC 11947);

...

Embodiment 2

[0032] Embodiment 2, the preparation of bacterial agent

[0033] The bacterial agent was prepared with the composite bacterial system described in Example 1.

[0034] One, the preparation of bacterial agent I

[0035] (1) Preparation of composite bacterial strain culture:

[0036] Inoculate Alcaligenes faecalis DSM 16503 in peptone cellulose culture medium (PCS) modified medium, culture statically at room temperature (30°C) and in the dark for 1 day, and collect all the fermentation broth as culture A;

[0037] Inoculate Bacteroides xylanisolvens DSM 18836 in peptone cellulose culture solution (PCS) modified medium, culture statically at room temperature (30°C) and in the dark for 1 day, and collect all the fermentation broth as culture C;

[0038] Inoculate Clostridium xylanolyticum DSM 6555T in the modified peptone cellulose broth (PCS) culture medium, culture statically at room temperature (30°C) and in the dark for 1 day, and collect all the fermentation broth as culture...

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PUM

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Abstract

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

Description

technical field [0001] The invention relates to an enzyme and bacterial agent for decomposing lignocellulose. Background technique [0002] Composed of lignin, hemicellulose and cellulose, lignocellulose is a potentially renewable resource that can be degraded to produce disaccharides or monosaccharides. Monosaccharide itself is a kind of energy substance, which can also be processed into other energy substances such as alcohol. Lignocellulose accounts for more than 50% of the carbon content in the plant kingdom, and its sources are extensive, such as cotton, wood, hemp, wheat straw, straw, bagasse, etc. are rich sources of cellulose. In today's energy shortage situation, degrading lignin can not only produce energy substances, but also turn waste into treasure, which is a double win. [0003] In recent years, lignocellulose degradation has become a research hotspot. Methods for degrading lignocellulose include hydrolysis, oxidation, and biodegradation. For the biodegrad...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12P19/14C12R1/05C12R1/145C12R1/20C12R1/38C12R1/01
Inventor 崔宗均郭鹏王小芬
Owner CHINA AGRI UNIV
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