Enzyme and microbial inoculum for decomposing lignocellulose

Abstract

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

Description

technical field

[0001] The invention relates to an enzyme and bacterial agent for decomposing lignocellulose. Background technique

[0002] Composed of lignin, hemicellulose and cellulose, lignocellulose is a potentially renewable resource that can be degraded to produce disaccharides or monosaccharides. Monosaccharide itself is a kind of energy substance, which can also be processed into other energy substances such as alcohol. Lignocellulose accounts for more than 50% of the carbon content in the plant kingdom, and its sources are extensive, such as cotton, wood, hemp, wheat straw, straw, bagasse, etc. are rich sources of cellulose. In today's energy shortage situation, degrading lignin can not only produce energy substances, but also turn waste into treasure, which is a double win.

[0003] In recent years, lignocellulose degradation has become a research hotspot. Methods for degrading lignocellulose include hydrolysis, oxidation, and biodegradation. For the biodegrad...