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Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof

A technology of sodium alginate and bacterial agents, applied in biochemical equipment and methods, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the problem of insufficient degradation efficiency of quinclorac and achieve applicable Wide range of media types, enhanced buffering capacity, and wide temperature adaptation range

Active Publication Date: 2016-07-13
四川润林肆汇中药材有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of optimal bacterial agent immobilization mode, i.e. sodium alginate composite immobilized bacterial agent, and disclose the preparation method and the use of this immobilized bacterial agent, solve the existing immobilization method to dichloroquine The problem that the degradation efficiency of phenolic acid is not high enough

Method used

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  • Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof
  • Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof
  • Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0041] The sodium alginate composite immobilized bacterial agent of the present invention comprises bacterial strains, sodium alginate, calcium chloride, an adsorption carrier composed of corncobs, bamboo charcoal and oil dryness, and an inorganic salt liquid medium.

[0042]In this embodiment, the bacterial strain is Pseudomonas stutzeri purchased from the Guangdong Provincial Microbial Culture Collection Center, and its number is GIM1.446. Since the above-mentioned Pseudomonas stutzeri is used, the present invention preferably uses The inorganic salt liquid medium for bacterial strain growth, the inorganic salt liquid medium is referred to as MSM, the composition of the MSM includes: FeCl 3 0.15g, MnCl 2 0.15g, NaNO 3 0.5g, K 2 HPO 4 1.70g, CaCl 2 ﹒ 2H 2 O0.01g, KH 2 PO 4 1.50g, FeSO 4 ﹒ 7H 2 O0.04g, MgSO 4 ﹒ 7H 2 O0.2g, (NH4) 2 SO 4 1.0g, pH﹦7, H 2 O1000ml.

[0043] The concrete preparation method of immobilized bacterial agent of the present invention is ...

Embodiment 2

[0061] In this example, the immobilized bacterial agent prepared in Example ③ composition ratio in Example 1 is used, and the inoculum amount is 10%. Then the effects of blank pellets, dispersed carriers, free bacteria, bacteria-containing carriers, and sodium alginate-fixed bacteria agents on the removal of quinclorac from soil samples were investigated. The difference between the above dosage form and the immobilized bacterial agent is only that: the blank pellet does not contain the bacterial mother liquid; the dispersed carrier refers to the adsorption carrier in the immobilized bacterial agent; the bacterial carrier refers to the adsorption carrier with the bacterial mother liquid; Sodium acid fixative does not contain adsorption carrier.

[0062] The specific detection method is: remove the gravel and pass through a 3mm sieve to measure the content of quinclorac, and add quinclorac after sieving to make the content reach 1000mg.kg -1 After stabilizing for 5 days, measur...

Embodiment 3

[0067] This embodiment adopts the immobilized bacterial agent made in the composition ratio of example ③ in Example 1, and uses it to detect the removal rate of quinclorac at different temperatures and different initial pH concentrations. The detection method is: 10% of the immobilized bacteria Bacteria and 3% free bacteria were added to MSM containing 800mg / L quinclorac respectively, cultured on a shaker at 140r / min, and the content of quinclorac was measured at regular intervals. The effects of temperature 15℃, 20℃, 25℃, 30℃, 35℃ and initial pH of 4, 5, 6, 7, 8, 9 on the degradation effect were investigated respectively. When examining the influence of temperature, the initial pH is 7, and when examining the initial pH, the temperature is 30°C, and the test results are as follows figure 2 and image 3 shown.

[0068] pass figure 2 It can be seen that:

[0069] The degradation rate of the immobilized bacterial agent was the highest when the temperature was 30℃, reaching...

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Abstract

The invention discloses a sodium alginate compound immobilized microbial agent and a preparation method thereof, and the sodium alginate compound immobilized microbial agent solves the problem that an immobilization way of pseudomonas suitable for di-chlorine quinoline acid degradation in the prior art is absent. A carrier culture medium with the pseudomonas and sodium alginate are mixed to obtain a mixture, and the mixture is dropwise added into calcium chloride with concentration being 1-4% to stand for 2-4 hours to prepare a granule; the mass concentration of the sodium alginate is 2-6%; the carrier culture medium comprises an adsorption carrier and an inorganic salt liquid culture medium; a weight ratio of the adsorption carrier to the sodium alginate is (1-3) to 1; and the adsorption carrier consists of corn cob, bamboo charcoal and oil cake in a weight ratio being 1:(1-2):1. According to the invention, the degradation efficiency of bacteria is effectively improved, and the degradation rate of the immobilized microbial agent is much higher than the sum of the physical absorption efficiency of blank spheres and biological degradation efficiency of a free bacterial strain, so that the effect of mutual promotion can be achieved.

Description

technical field [0001] The invention relates to a bacterial agent, in particular to a sodium alginate composite immobilized bacterial agent and its preparation method and application. Background technique [0002] my country is one of the big countries in the production and use of pesticides. The annual amount of pesticides used is as high as 500,000-600,000 tons, including herbicides, insecticides and fungicides. Due to the low utilization efficiency of pesticides in my country, a large amount of pesticides flow into the environment. Pesticides not only increase crop yield, but also pose a threat to food safety, so they have been widely concerned by researchers in recent years. [0003] Quinclorac is a selective post-emergence treatment herbicide, which belongs to hormone-type quinoline carboxylic acid agents, and is mainly used to control barnyardgrass and other gramineous weeds in rice fields, and is widely used in agricultural production in my country , the agent is stab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C02F3/34B09C1/10C12R1/38C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N11/04C12N11/10
Inventor 张可陈强罗鸿兵陈伟陈佳
Owner 四川润林肆汇中药材有限公司
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