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57results about How to "Stable living environment" patented technology

Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof

The invention discloses a sodium alginate compound immobilized microbial agent and a preparation method thereof, and the sodium alginate compound immobilized microbial agent solves the problem that an immobilization way of pseudomonas suitable for di-chlorine quinoline acid degradation in the prior art is absent. A carrier culture medium with the pseudomonas and sodium alginate are mixed to obtain a mixture, and the mixture is dropwise added into calcium chloride with concentration being 1-4% to stand for 2-4 hours to prepare a granule; the mass concentration of the sodium alginate is 2-6%; the carrier culture medium comprises an adsorption carrier and an inorganic salt liquid culture medium; a weight ratio of the adsorption carrier to the sodium alginate is (1-3) to 1; and the adsorption carrier consists of corn cob, bamboo charcoal and oil cake in a weight ratio being 1:(1-2):1. According to the invention, the degradation efficiency of bacteria is effectively improved, and the degradation rate of the immobilized microbial agent is much higher than the sum of the physical absorption efficiency of blank spheres and biological degradation efficiency of a free bacterial strain, so that the effect of mutual promotion can be achieved.
Owner:四川润林肆汇中药材有限公司

Microfluidic cell culture chip guaranteeing uniform cell distribution and cell sample introduction method

The invention discloses a microfluidic cell culture chip guaranteeing uniform cell distribution and a cell sample introduction method. A main flow channel and auxiliary flow channels which are cross and vertical to the main flow channel are formed in a substrate, each auxiliary flow channel is a step-shaped flow channel which is granularly widened from the outer end to the middle, and the bottom surfaces of the auxiliary flow channels are higher than the bottom surface of the main flow channel; firstly, a culture solution is injected into a culture chamber, a cell suspension solution is injected into the culture chamber after the bottom of the culture chamber is filled with the culture solution, the culture solution is injected into the main flow channel after a certain quantity of the cell suspension solution is injected into the main flow channel, the culture solution pushes the injected cell suspension solution to the culture chamber, mixed gas enters buffer zones on two sides of the culture chamber through the auxiliary flow channels, the bottom of the culture chamber in the middle of the main flow channel is enabled to be filled with the cell suspension solution while the other space of the main flow channel is filled with the culture solution, therefore, cells are just distributed at the bottom of the culture chamber, no cells remain in the non-culture-chamber zone, and the cells are uniformly distributed at the bottom of the culture chamber.
Owner:南京九川科学技术有限公司

Culture apparatus and method for complete autotrophic nitrogen removal granule sludge

The invention discloses a culture apparatus and a method for complete autotrophic nitrogen removal granule sludge, relating to the technical field of biological sewage treatment, in particular to the treatment field of nitrogen pollution. The culture apparatus and the culture method are used for solving the problems that the anaerobic ammonia oxidation nitrogen removal method has low nitrogen removal efficiency and poor running stability due to low sludge concentration and poor sludge settling effect caused by small sludge flocculation particle size in the existing complete autotrophic nitrogen removal method based on anaerobic ammonia oxidation. The culture apparatus comprises a U-shaped reactor main body, a heat preservation system, a water inflowing system, a drainage system, an aerating system, an automatic control system and a parameter monitoring system. A situation that the whole culture process can be controlled and monitored intelligently is achieved by connection of all parts and setting of a culture environment, strong aeration is carried out firstly during culturing to enable the sludge to be granulated, and then anaerobic culture is performed on anaerobic ammonium oxidation bacteria to culture the complete autotrophic nitrogen removal granule sludge successfully. The culture apparatus and the culture method are suitable for the sewage treatment field.
Owner:HARBIN INST OF TECH

Vertical multistage AO ecological sewage treatment system

The invention relates to a vertical multistage AO ecological sewage treatment system. The vertical multistage AO ecological sewage treatment system is characterized in that sewage is orderly treated by a coarse screen, a lifting pump house, a fine screen, a grit basin, a vertical multistage AO ecological tank, a cloth filter tank and a UV disinfection canal and then is discharged by a standard discharge opening, and sludge produced by the fine screen, the grit basin, the vertical multistage AO ecological tank and the standard discharge opening is transferred into a sludge storage tank, then is treated in a dehydration machine room and can be carted. The treatment system has less sludge amount (of sludge-drainage time of 20-25 years), does not produce odor, realizes thorough removal of organic matters, provides a carbon source produced in sludge stabilization for denitrification demands, reduces an external carbon source, produces water satisfying first-grade standard A or B, can be used for construction of an ecological landscape park, has the advantages of less construction investment and short construction period, can utilize waste and old or existing pits, ponds, trenches and slopes, and has a construction period of 4-6 months.
Owner:FUJIAN GUANGHUILONG ENVIRONMENTAL PROTECTION TECH

Land-saving type efficient nitrogen and phosphorus removal sewage treatment device and method

The invention discloses a land-saving type efficient nitrogen and phosphorus removal sewage treatment device and method. The land-saving type efficient nitrogen and phosphorus removal sewage treatmentdevice comprises a pretreatment unit; the left end of the pretreatment unit is provided with a water inlet; the right end of the pretreatment unit is communicated with the left end of an anaerobic pool; the right end of the anaerobic pool is communicated with the left end of a first-stage aerobic pool; the right end of the first-stage aerobic pool is communicated with the left end of a secondarysedimentation pool; the right end of the secondary sedimentation pool is communicated with the left end of an anoxic pool; the right end of the anoxic pool is communicated with the left end of a secondary-stage aerobic pool; the right end of the secondary-stage aerobic pool is communicated with the left end of a deep treatment unit; the right end of the deep treatment unit is provided with a wateroutlet; and the anoxic pool and the secondary-stage aerobic pool are both filled with a biological membrane filler. The traditional A / A / O technology is improved into combination of front section A / Oadopting an activated sludge method and post A / O adopting a pure biological membrane method; and compared with the activated sludge method and a sludge-membrane mixing technology in the past, the combination greatly saves the occupying area while strengthening the pollutant effect.
Owner:四川天润德环境工程有限公司 +1

Preparation and application of ammonia oxidizing bacteria immobilized bioactive filler based on polyurethane carrier

The invention discloses preparation and an application of an ammonia oxidizing bacteria immobilized bioactive filler based on a polyurethane carrier, and belongs to the technical field of water treatment. The bioactive filler comprises two parts: an embedding body and the polyurethane carrier; a porous structure of the polyurethane foam carrier adsorbs a bacteria-containing embedding liquid, wherein the embedding liquid is prepared by mixing an ammonia oxidizing bacteria concentrated solution and a polyvinyl alcohol solution; a whole sheet of polyurethane foam is used as the embedding carrier, the embedding liquid is immersed to the interior of the polyurethane foam by a squeezing way, the embedding body is formed after boric acid secondary cross-linking and is combined with the polyurethane carrier to obtain a filler shape meeting requirements, and thus the ammonia oxidizing bacteria bioactive filler is obtained. The prepared ammonia oxidizing bacteria immobilized bioactive filler not only solves the problems that ammonia oxidizing bacteria are difficult in advantage establishment, easy to lose and the like, but also improves the processing ability of a reactor, shortens the start-up time, and has broad application prospects.
Owner:BEIJING UNIV OF TECH

Local air-water reversing upflow biological aerated filter and sewage treatment method thereof

The invention discloses a local air-water reversing upflow biological aerated filter and a sewage treatment method thereof, and relates to a water treatment device and a water treatment method. According to the biological aerated filter, a needle punched nonwoven filter material is taken as a packing, and a plurality of aeration branch pipes are arranged in the height direction of an aeration main pipe. The water treatment method comprises the following steps of: regulating the pH value of sewage to be treated to be 7.5-8, pumping into the filter, pumping air into the filter, ensuring that the hydraulic retention time is 1 to 2 hours, and directly discharging the obtained supernate. Small-flow aeration of which the aeration direction is opposite to upward water flow, the water flow is disturbed on the water flow section, air and water are uniformly distributed, a mass transfer effect is good, phenomena of short flow and channeling can be reduced, anaerobic areas in a system can be effectively eliminated, a membrane is formed uniformly, a problem of poor nitrification effect during operation of the traditional biological aerated filter is solved to a great extent, and the average ammonia nitrogen concentration of effluent is 8.63mg / L. The invention is used for treating wastewater.
Owner:HARBIN INST OF TECH

Micro-fluidic gel gas-liquid interface flue gas exposing device

The invention discloses a micro-fluidic gel gas-liquid interface flue gas exposing device. The micro-fluidic gel gas-liquid interface flue gas exposing device comprises an upper-layer chip, a lower-layer chip and a middle-layer chip, wherein the upper-layer chip provides a flue gas inlet and outlet channel; the lower-layer chip provides a cell culture fluid flowing channel; the middle-layer chip is positioned between the upper-layer chip and the lower-layer chip and is sued for loading gel and cells on the gel; cell culture fluid in the lower-layer chip provides a living environment for cells in the middle-layer chip; and the gel in the middle-layer chip becomes a gas-liquid interface and enables the cells to be in direct contact with flue gas. The three layers of chips are combined, the cells are positioned on the gel and do not in direct contact with the cell culture fluid, and direct impact of the culture fluid to the surfaces of the cells is avoided; meanwhile, the culture fluid can be supplied for the cells through the gel, and stability of the living environment of the cells is kept; and on the basis, the cells can be in direct contact with flue gas, so that related biological information of the flue gas stimulating the cells is obtained.
Owner:NANJING UNIV +1

Bioreactor for treating high-salt organic wastewater based on composite salt-tolerant bacterial strain

The invention discloses a bioreactor for treating high-salt organic wastewater based on a composite salt-tolerant bacterial strain. The bioreactor comprises a plurality of anaerobic reactors and aerobic sewage treatment devices which are sequentially communicated in series through communicating pipes, a supporting pore plate is fixedly arranged at the lower end in each anaerobic reactor, a fillerparticle bed layer is laid on each supporting pore plate, salt-tolerant anaerobic bacteria are added into each anaerobic reactor, and a first water distribution pipe is laid at the bottom in each anaerobic reactor; when every two adjacent anaerobic reactors are communicated in series through a communicating pipe fitting, a water filter screen is arranged at an inlet in the front end of the communicating pipe fitting and horizontally extends into the upper end of a previous anaerobic reactor, and an outlet in the rear end of the communicating pipe fitting is communicated with the first water distribution pipe laid at the bottom of the interior of a next anaerobic reactor; after anaerobic treatment, the wastewater flows into an aerobic sewage treatment device for aerobic treatment. The bioreactor disclosed by the invention is relatively high in high-salt organic wastewater treatment efficiency and low in wastewater treatment cost.
Owner:浙江省天正设计工程有限公司

Double sludge oxidation ditch denitrification phosphate-removing apparatus and method thereof

The invention discloses a double sludge oxidation ditch denitrification phosphate-removing apparatus and a method thereof. The apparatus comprises an oxidation ditch; an anaerobic zone, an anoxic zone and a water outlet zone which are communicated in order are arranged in the oxidation ditch, an independent nitrification zone is arranged between the anaerobic zone and the anoxic zone along the sewage flowing direction, a pre-anaerobic zone is arranged between the anoxic zone and the anaerobic zone, the independent nitrification zone is separated with the anaerobic zone and the anoxic zone through a first baffle plate and a second baffle plate, a first mud-separation zone, a filling material zone, a second mud-separation zone and a current stabilization zone are arranged in order along the sewage flowing direction, and nitrobacteria is enriched in the filling material zone. The denitrification phosphate-removing method is characterized in that a main channel of the oxidation ditch is always in an anaerobic/anoxic state, so that the denitrifying phosphorus-removing bacteria can be a dominant bacteria, the filling material is employed for hanging a membrane in the independent nitrification zone, so that a stable living environment is provided for nitrobacteria, nitrobacteria biomass in the independent nitrification zone is increased, nitrification rate is increased, and denitrification phosphate-removing effect can be guaranteed.
Owner:WUHAN UNIV OF TECH

Fish releasing device for water conservancy and hydropower engineering

The invention discloses a fish releasing device for water conservancy and hydropower engineering. The device comprises a pipeline and a fish carrying tank; a flow dividing pipe is arranged under the pipeline, and one side of the flow dividing pipe is provided with flow dividing through pipes; one side of the fish carrying tank is provided with an oxygen bottle, one side of vertical plates is provided with a push-pull plate, and the outer surface of the push-pull plate is provided with a control valve; the inner surface of the fish carrying tank is provided with a heat insulating layer, one side of the heat insulating layer is provided with a partition plate, and the partition plate is fixedly connected with the fish carrying tank through the heat insulating layer; two sides of the partition plate are each provided with buoyancy balls and flexible lines; and the outer surface of the heat insulating layer is provided with fixing bolts. According to the fish releasing device for the waterconservancy and hydropower engineering disclosed by the invention, an anti-slip mat layer, the buoyancy balls and the flexible lines are arranged, so that stability of the whole device can be increased, stability of the living environment of fishes can be maintained, and the fishes can be prevented from jumping out of the fish carrying tank and being hurt; and the device has good ventilation, issuitable for different working conditions, and brings better use prospects.
Owner:傅逅荣

Sea cucumber seedling culture water temperature constant system and use method thereof

The invention discloses a sea cucumber seedling culture water temperature constant system and a using method thereof, and belongs to the technical field of mariculture. The sea cucumber seedling culture water temperature constant system comprises a first water inlet pipeline, a heating pipeline assembly, a cooling pipeline assembly, a second water inlet pipeline, and a control assembly; the heating pipeline assembly is mounted on one water outlet of the first water inlet pipeline; the cooling pipeline assembly is mounted on the other water outlet of the first water inlet pipeline; the second water inlet pipeline is connected with the heating pipeline assembly and the cooling pipeline assembly; and the control assembly is connected to the first water inlet pipeline, the heating pipeline assembly, the cooling pipeline assembly and the second water inlet pipeline and used for executing on-off of a water flow and regulation and control of the temperature of the water flow. According to the sea cucumber seedling culture water temperature constant system, the heating temperature and a stroke of the water flow can be calculated according to the temperature and humidity change of the surrounding environment, heat loss in the water flowing process is compensated, and the water temperature can be accurately controlled.
Owner:烟台市海洋经济研究院

Nucleus pulposus progenitor cell culture medium and preparation method and application thereof

ActiveCN111019896AExcellent self-renewal abilitySimple stepsCulture processNervous system cellsTransferrinBiophysics
The invention provides a nucleus pulposus progenitor cell culture medium as well as a preparation method and an application thereof. The nucleus pulposus progenitor cell culture medium consists of a DMEM culture medium, an F12 culture medium, proline, sodium pyruvate, vitamin C, an insulin growth factor, FGF2, transferrin, a ROCK inhibitor, a p38 inhibitor and a transforming growth factor beta signal channel inhibitor. The preparation method comprises the following steps: mixing the components, adjusting the pH and osmotic pressure of the mixture, and performing sterilizing. The culture mediumdisclosed by the invention does not use animal-derived components; a undifferentiated and pluripotent state of the NPPCs can be maintained; the removal of animal serum in the culture medium greatly avoids the interference and pollution of animal components to nucleus pulposus progenitor cell culture, can completely avoid the induction of immune response in the cell treatment process, simplifies the culture process, increases the credibility of experimental results, and basically solves the key problem of durable culture of NPPCs. The preparation method is simple, raw materials are easy to obtain, and the culture medium is suitable for culture and amplification of nucleus pulposus progenitor cells under feeder-free cell conditions.
Owner:ZHEJIANG UNIV

Planting method of non-toxic rice

The invention provides a planting method for non-toxic rice, and belongs to the technical field of agriculture. The planting method comprises the following steps of preparing a base fertilizer, preparing a paddy field, carrying out paddy field arrangement, planting the rice, and carrying out normal field management, and also comprises the following steps of carrying out pretreatment on the paddy field, carrying out sowing, carrying out field management before seedling pulling, transplanting rice seedlings, carrying out field management in the growth period, carrying out treatment before harvest, and carrying out harvesting. According to the method, a traditional Chinese medicine is used for sterilization and deinsectization, so that the effects of sterilization and deinsectization are achieved under the condition that no chemical pesticides are used, meanwhile, a greenhouse and a temperature control and water control system are used for carrying out controlled planting on the rice in the paddy field, so that the interference of outdoor plant diseases and insect pests to the rice is isolated, meanwhile, the growth environment of the rice can be improved and automatically controlled,the yield of the rice is increased, in addition, a base fertilizer body and traditional Chinese medicine residues are adopted as the base fertilizer, so that soil is improved, salinization of the soil is avoided, and the traditional Chinese medicine residues can be further used as a fertilizer to promote the growth of the rice when being rotted and decomposed.
Owner:兴业县嘉沃生态农业科技有限责任公司

Trigona rearing box and application method

The invention discloses a trigona rearing box. The trigona rearing box comprises a box bottom, the top of the box bottom is provided with a pollen storage box, the top of the pollen storage box is provided with a breeding box, the top of the breeding box is provided with a honey storage box, and the top of the honey storage box is provided with a box cover. The top of the box cover is provided with a protection assembly with sun-proof and rain-proof functions, a first inner box layer is arranged in the pollen storage box, the portion between the inner wall of the pollen storage box and the outer wall of the first inner box layer is filled with a first thermal insulation layer, the inner wall of the first inner box layer is fixedly connected with a plurality of annular pollen storage plates, a second inner box layer is arranged in the breeding box, the portion between the inner wall of the breeding box and the outer wall of the second inner box layer is filled with a second thermal insulation layer, and a plastic honey box is arranged in the honey storage box. According to the trigona rearing box, a trigona comb is functionally partitioned by the pollen storage box, the breeding boxand the honey storage box, the multiple layers of boxes are combined and stacked, the boxes are disassembled to be recombined, and swarming is achieved. Honey is quickly produced through the honey box, and the damage to a swarm is reduced; airflow channels are formed, it is ensured that trigona is normally bred, the feeding box conforms to the life habits of the trigona, and therefore, the production efficiency is improved.
Owner:云南省农业科学院蚕桑蜜蜂研究所
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