Choice for preventing endogenic bacterial strain by potato

Abstract

A select method for strain preventing and curing endotrophic mycorrhiza in potato. Detect width of bacteriostasis braid and select strains with good effects of endotrophic mycorrhiza, do pot incubation test and field practice, detect effects of prevention and cure and fix planting ability. Detect action of the strain to pathogenetic fungi, and induct antiviral ability to potato. Advanced strain is obtained after the steps.

Description

[0001] technical field

[0002] The invention relates to a long-term symbiosis between endophytic bacteria and plants, which can slow down the defense response of host plants, and a method for selecting endophytic bacterial strains for biological control of potatoes. Background technique

[0003] Most of the biocontrol bacteria for plant diseases are isolated from the soil, rhizosphere or plant surface, and most of the bacteria with antagonistic effect determined indoors cannot colonize normally on the host surface or in vivo when applied outdoors, and are affected by the environment. Factors such as changes in conditions and changes in plant rhizosphere microbial populations under different ecological conditions make their biocontrol effects unstable and sometimes even ineffective.

[0004] Production is mainly based on chemical control, which not only makes it difficult for the medicine to be effective for a long time, but also seriously pollutes the environment. Potatoes a...

Examples

Embodiment Construction

[0025] 1. Wash the healthy potato roots, stems and leaves to be separated, dry them, soak them in 75% alcohol for 2-5 minutes, soak them in 0.1% mercury liter for 1-2 minutes, wash them with sterilized water for 4 times, add sterilized water Grind a small amount, let it stand for 10-15min, take the bacterial suspension with a curved glass rod and apply it on the plate, and coat two plates continuously. At the same time, smear the plate with the sterilized water used to wash the isolated material for the last time. If colonies grow on the plate in the future, discard the isolated strain. Cultivate in an incubator at 28°C for 48 hours. Pick a single colony for purification, and transfer the pure strain to a tube for storage until use.

[0026] 2. Plate antagonism test: inoculate the endophytic bacteria of potato and Phytophthora potato oppositely on the rye peptone medium plate, and measure the bacteriostatic bandwidth two days later. The strains with antibacterial effect were ...

[0001] technical field

[0002] The invention relates to a long-term symbiosis between endophytic bacteria and plants, which can slow down the defense response of host plants, and a method for selecting endophytic bacterial strains for biological control of potatoes. Background technique

[0003] Most of the biocontrol bacteria for plant diseases are isolated from the soil, rhizosphere or plant surface, and most of the bacteria with antagonistic effect determined indoors cannot colonize normally on the host surface or in vivo when applied outdoors, and are affected by the environment. Factors such as changes in conditions and changes in plant rhizosphere microbial populations under different ecological conditions make their biocontrol effects unstable and sometimes even ineffective.

[0004] Production is mainly based on chemical control, which not only makes it difficult for the medicine to be effective for a long time, but also seriously pollutes the environment. Potatoes a...

Examples

Embodiment Construction

[0025] 1. Wash the healthy potato roots, stems and leaves to be separated, dry them, soak them in 75% alcohol for 2-5 minutes, soak them in 0.1% mercury liter for 1-2 minutes, wash them with sterilized water for 4 times, add sterilized water Grind a small amount, let it stand for 10-15min, take the bacterial suspension with a curved glass rod and apply it on the plate, and coat two plates continuously. At the same time, smear the plate with the sterilized water used to wash the isolated material for the last time. If colonies grow on the plate in the future, discard the isolated strain. Cultivate in an incubator at 28°C for 48 hours. Pick a single colony for purification, and transfer the pure strain to a tube for storage until use.

[0026] 2. Plate antagonism test: inoculate the endophytic bacteria of potato and Phytophthora potato oppositely on the rye peptone medium plate, and measure the bacteriostatic bandwidth two days later. The strains with antibacterial effect were ...