Compound composition for preventing and treatment of grape diseases and application thereof

A compound composition, a technology for grape white rot, applied in the application, biocide, animal repellent and other directions, can solve the problem of drug resistance of bacteria, reduce the input amount, achieve remarkable control effect, and avoid drug resistance. Effect

Inactive Publication Date: 2018-05-11
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term single use of imazalil in production, drug-resistant groups continue to appear, and it is easy to induce drug resistance in bacteria

Method used

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  • Compound composition for preventing and treatment of grape diseases and application thereof
  • Compound composition for preventing and treatment of grape diseases and application thereof
  • Compound composition for preventing and treatment of grape diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Indoor toxicity test and compound ratio screening

[0023] 1.1 Drugs to be tested

[0024] 0.3% eugenol soluble agent, product of Shandong Yijia Agrochemical Co., Ltd., dissolved in water to make 1000mg / L mother liquor for later use.

[0025] 98% Imazalil original drug, provided by Yifan Biotechnology Group Co., Ltd. Dissolve it in acetone first, then make it into 10000 mg / L mother liquor at constant volume for later use.

[0026] 1.2 Tested strains and host plants

[0027] Grape white rot (Coniothyrium diplodiella) was isolated from diseased fruit in the field, and the first-generation strain obtained by monospore culture and purification was stored in a 4°C refrigerator for later use.

[0028] 1.3 Experimental method

[0029] The Mycelium growth rate test recommended by the FAO was used.

[0030] 1.3.1 Preparation of drug-containing tablet

[0031] 1.3.1.1 Drug compounding scheme

[0032] Table 1 Eugenol and Imazalil Compound Ratio

[0033]

[0034] Table 2...

Embodiment 2

[0063] field plot experiment

[0064] According to the indoor compounding ratio screening experiment, the optimal ratio of eugenol:imazalil=1:20 was selected, and we prepared 4.2% eugenol·imazalil composition.

[0065] 2.1 Experimental drug and community arrangement

[0066] According to the "Guidelines for Field Tests of Pesticides (2)" edited by the Ministry of Agriculture's Pesticide Control Institute, Part 121: Fungicides for Grape White Rot Control, "Part 13 of "Guidelines for Field Tests for Pesticides": Fungicides for Control of Grapes Anthracnose" designed a field efficacy test of 4.2% eugenol·imazalil on grape white rot and anthracnose.

[0067] 2.1.1 Drugs to be tested

[0068] 0.3% eugenol soluble solution 600 times, product of Shandong Yijia Agrochemical Co., Ltd.

[0069] 10% Imazalil water solution 800 times, provided by Belgium Janssen Pharmaceutical Company.

[0070] 4.2% Eugenol · Imazalil 800, 900, 1000 times, prepared by Yantai Academy of Agricultural Sc...

Embodiment 3

[0110] safety test

[0111] The content of imazalil in the compound composition is 6 percentage points lower than that of commercially available products, so its toxicity to humans and animals and the safety to crops meet the requirements; the content of eugenol in the compound composition is 0.2%. The results of the toxicity test of the accreditation agency show that the compound drug belongs to the slightly toxic pesticide:

[0112] Rat acute oral toxicity LD50♂>5000mg / kg,♀>5000mg / kg;

[0113] Rat acute dermal toxicity LD50♂>5000mg / kg,♀>5000mg / kg;

[0114] Rabbit eye irritation score is 0 (1h), after 48h it is 0 (1:100) dilution, no irritation to eyes;

[0115] Rabbit eye irritation score is 0 (4h), non-irritating to the skin.

[0116] The present invention establishes the optimal ratio of the two pesticides for use in production through indoor toxicity testing and field tests on the effects of the biological source pesticides eugenol and imazalil on grape white rot and a...

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PUM

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Abstract

The invention discloses application of a compound composition of eugenol and imazalil in the agricultural field, and particularly relates to application research special for prevention and control ofgrape white rot and anthracnose. The invention provides eugenol and application of the compound composition of eugenol and imazalil in prevention and treatment of grape white rot and anthracnose. In the compound composition, the weight ratio of eugenol to imazalil is 20:1-1:60, the synergism proportion is 1:(1-40), and the optimal proportion is 1:20. The compound composition has remarkable performance in the prevention and treatment of grape white rot and anthracnose.

Description

technical field [0001] The invention relates to a compound composition containing eugenol, especially the compound composition of eugenol and imazalil, which is used as a fungicide to prevent and control grape white rot and anthracnose, and belongs to the compound composition for preventing and controlling grape diseases and its technical fields of application. Background technique [0002] Grape white rot (Coniella diplodiella) and anthracnose (Colletotrichumgloeosporioides) are important fruit diseases in various grape producing areas in my country. The incidence is increasing and it is extremely common. Every year, due to improper management or untimely control, the loss of grape production is as high as 20%- 40%, up to 80% or more in severe years, or even no harvest, which directly affects the economic income of fruit farmers. [0003] At present, chemical agents are mainly used to prevent and control the above-mentioned fruit diseases, but in the process of prevention a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/50A01N31/16A01P3/00
CPCA01N31/16A01N43/50
Inventor 李宝燕王英姿王培松刘保友栾炳辉
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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