Production technology of high-density composite nitrobacterium inoculant, and high-density fermentation batch culture fermentation tank system of nitrobacteria

A technology of nitrifying bacteria and production process, applied in the field of environmental biology, can solve the problems of lack of production process, hinder the productization and commercialization process of nitrifying bacteria, etc., to increase the biomass of nitrifying bacteria, reduce the sedimentation separation tank and flocculation stirring tank, The effect of increasing cell density and resistance

Active Publication Date: 2017-03-22
HUATIAN ENG & TECH CORP MCC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the lack of production technology for high-density nitrifying bacteria inoculum hinders the productization and commercialization of nitrifying bacteria

Method used

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  • Production technology of high-density composite nitrobacterium inoculant, and high-density fermentation batch culture fermentation tank system of nitrobacteria
  • Production technology of high-density composite nitrobacterium inoculant, and high-density fermentation batch culture fermentation tank system of nitrobacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1) Seed culture of nitrifying bacteria: take nitrifying sludge from urban sewage treatment plant and inoculate it in a 1L Erlenmeyer flask with an inoculum size of 10%. The Erlenmeyer flask is filled with 300mL medium, and each liter of medium contains: NaHCO3 1.5g, CH 3 COONa 0.5g, (NH4) 2 SO 4 5.0g, NaCl 1.0g, FeSO4 0.2g, K 2 HPO 4 1.0g, MgSO 4 0.25g, used as seed liquid after culturing for 5 days;

[0044] 2) Preparation by co-precipitation method: take 43.2g FeCl 3 ·6H 2 O and 24.70 g FeCl 2 4H 2 O was added to a three-necked flask, and mixed with an appropriate amount of sodium citrate (final concentration: 0.02mol / L) as a dispersant, and placed on a constant temperature heating magnetic stirrer. Under the protection of nitrogen, heat up to 60°C, 1000r / min, stir at constant temperature for 8-10min, then add 2mol / L NaOH solution dropwise until the pH of the solution is 11, continue to react for 60min, and cool down to room temperature naturally. Wash thr...

Embodiment 2

[0053] 1) Seed culture of nitrifying bacteria: Take nitrifying sludge from urban sewage treatment plant and inoculate it in a 1L conical flask with an inoculum size of 10%. The conical flask is filled with 300mL medium, and each liter of medium contains: NaHCO 3 1.5g, CH 3 COONa 0.5g, (NH 4 ) 2 SO 4 5.0g, NaCl 1.0g, FeSO 4 0.2g,K 2 HPO 4 1.0g, MgSO4 0.25g, used as seed liquid after culturing for 5 days;

[0054] 2) Preparation by co-precipitation method: take 43.2g FeCl 3 ·6H 2 O and 24.70 g FeCl 2 4H 2 O was added to a three-necked flask, and mixed with an appropriate amount of sodium citrate (final concentration: 0.02mol / L) as a dispersant, and placed on a constant temperature heating magnetic stirrer. Under the protection of nitrogen, heat up to 60°C, 1000r / min, stir at constant temperature for 8-10min, then add 2mol / L NaOH solution dropwise until the pH of the solution is 11, continue to react for 60min, and cool down to room temperature naturally. Wash thre...

Embodiment 3

[0061] Embodiment 3 composite nitrifying bacteria bacterial agent is to the ammonia nitrogen degradation of urban water body

[0062] 1) Refer to steps 1-6 of Example 2 to obtain a composite nitrifying bacteria bacterial agent.

[0063] 2) Take the surface water from Donghu Lake in Maanshan City. The main water quality parameters COD, BOD, ammonia nitrogen, total nitrogen, and total phosphorus concentration are 74-139mg / L, 34.8-41mg / L, 6.47-12.16mg / L, 9.50-13.2mg / L, respectively. L, 0.35~0.86mg / L. Add dry powder of compound nitrifying bacteria agent to 2000-5000mL test water at a concentration of 2-4mg / L, aerate and oxygenate to ensure that the dissolved oxygen concentration in the water is not less than 2mg / L, and after 5-7 days, the ammonia nitrogen in the water will degrade The rate reaches 91.2-98.7%, and the turbidity of the water body drops from the original 22.6-23.9 NTU to 8.4-8.6 NTU (NTU is equivalent to 1 mg of SiO in 1 L of water. 2 Or when it is unbent kaolin or...

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Abstract

The invention discloses a high-density batch culture production technology of a nitrobacterium inoculant for degrading ammonia nitrogen in water, and a high-density fermentation batch culture fermentation tank system of nitrobacteria, used for the technology. The production technology comprises the steps of magnetic nano-powder preparation, nitrobacterium seed liquid culture, magnetically carried nitrobacterium immobilization, batch fermentation culture, flocculation mixed precipitate magnetic separation, freeze drying and preparation compounding. The production technology improves the effective cell density of a nitrobacterium preparation, allows a flocculating agent and a stimulant to be compounded while preparing the nitrobacteria inoculant, and the nitrobacterium inoculant can be used in water restoration, can improve the transparence of water, and guarantees the sufficient nitrobacterium cell density required by water restoration and adaptability to new environment.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and relates to a production process of a bacterial agent and a batch culture fermenter used for high-density fermentation of nitrifying bacteria in the above-mentioned process. Background technique [0002] Various point and non-point source pollution such as urban domestic sewage, industrial wastewater, agricultural sewage, domestic garbage, and initial rainwater have invaded urban water bodies, and the water environment of urban rivers has been polluted to varying degrees. Class IV standard in Environmental Quality Standard (GB3838-2002) (mainly applicable to general industrial water areas and recreational water areas with indirect contact with human body), the water body presents a state of eutrophication, especially the excess of ammonia nitrogen pollutants will cause planktonic The vigorous reproduction of plants consumes the dissolved oxygen in the water, which directly leads ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12M1/42C12M1/34C12M1/12C12M1/04C12M1/02C12M1/00
CPCC12M27/02C12M29/06C12M37/02C12M41/00C12M41/22C12M41/26C12M41/32C12M41/42C12M41/48C12M47/02C12N1/02C12N1/20
Inventor 赵丹程寒飞孟溪冯植飞
Owner HUATIAN ENG & TECH CORP MCC
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