Biogel for treating joint diseases and application thereof
A biological gel, joint disease technology, applied in the field of biomedicine, to achieve the effect of maintaining cell growth, prolonging the effective time, and prolonging the growth time
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Embodiment 1
[0047] The preparation of embodiment 1 Chinese medicine extract (one)
[0048] 1. Accurately weigh the fully dried raw materials, including 8 parts of hibiscus flower, 3 parts of licorice, 15 parts of reed root, and 7 parts of chrysanthemum, soak them in 80% ethanol for 60 minutes, and grind them with a tissue breaker.
[0049] 2. Ultrasonic crushing, power 600W, time 30 minutes, 12000rpm centrifugation 15min, take the supernatant, and then dilute with ethanol.
[0050] 3. Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 5 times in total.
[0051] 4. Drain the recovered n-butanol solution with a negative pressure centrifuge, and dissolve the residue with absolute ethanol.
[0052] 5. Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water until the t...
Embodiment 2
[0053] The preparation of embodiment 2 Chinese medicine extract (two)
[0054] 1. Accurately weigh the fully dried raw materials, including 10 parts of hibiscus flower, 5 parts of licorice, 10 parts of reed root, and 5 parts of chrysanthemum, soak them in 80% ethanol for 50 minutes, and grind them with a tissue breaker.
[0055] 2. Ultrasonic disruption, power 500W, time 40 minutes, 15000rpm centrifugation 10min, take the supernatant, and then dilute with ethanol.
[0056] 3. Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 5 times in total.
[0057] 4. Drain the recovered n-butanol solution with a negative pressure centrifuge, and dissolve the residue with absolute ethanol.
[0058] 5. Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water until th...
Embodiment 3
[0059] The preparation of embodiment 3 Chinese medicine extract (three)
[0060] 1. Accurately weigh the fully dried raw materials, including 5 parts of hibiscus flower, 2 parts of licorice, 20 parts of reed root, and 10 parts of chrysanthemum, soak them in 80% ethanol for 70 minutes, and grind them with a tissue breaker.
[0061] 2. Ultrasonic crushing, power 600W, time 50 minutes, centrifugation at 10000rpm for 20min, take the supernatant, and then dilute with ethanol.
[0062] 3. Extract with water-saturated n-butanol equal to the volume of the constant volume solution, and extract 4 times in total;
[0063] 4. Drain the recovered n-butanol solution with a negative pressure centrifuge, and dissolve the residue with absolute ethanol.
[0064] 5. Take 1ml of the product, extract it with petroleum ether, and make a standard curve with astragalus flavonoids. The product is accurately quantified by the absorbance method, and the remaining product is diluted with sterile water u...
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