Bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis

A technology of Bacillus subtilis and corn leaf spot, applied in the field of microorganisms, can solve the problems of lack of application of Bacillus subtilis, and achieve the effects of reducing the occurrence of corn leaf spot, strong antibacterial properties, and strong stress resistance

Active Publication Date: 2016-07-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is a lack of a Bacillus subtilis with strong ...

Method used

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  • Bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis
  • Bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis
  • Bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis

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Embodiment 1

[0038]A kind of Bacillus subtilis bacterial strain of the present invention, this bacterial strain is DZSY21; Preservation name is Bacillus subtilis; Described Bacillus subtilis DZSY21 (Bacillus subtilis DZSY21) is collected from the Eucommia plant leaf tissue of Anhui Agricultural University campus in Hefei City, Anhui Province It was obtained by isolation of endophytic bacteria and microorganisms, and was preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; preservation date: November 2015 27th; deposit number: CGMCCNO.11749.

[0039] The 16SrDNA gene sequence of the Bacillus subtilis is the nucleotide sequence shown in SEQIDNo.1, and the gyrB gene sequence of the Bacillus subtilis is the nucleotide sequence shown in SEQIDNo.2;

[0040] The single bacterium colony of described Bacillus strain is round on the beef extra...

Embodiment 2

[0052] The difference between embodiment 2 and embodiment 1 is:

[0053] The preparation method of bacillus subtilis bacterial agent of the present invention, comprises the steps:

[0054] In step (1), preparation of Bacillus subtilis seed liquid: Bacillus subtilis DZSY21 was inoculated in the culture solution, 50 mL of the culture solution was placed in a 250 mL conical flask, the stirring rate was 160 r / min, and cultured at 36° C. for 14 hours, to culture medium OD 600 0.9, the seed solution was obtained; the formula of the culture solution was: 10g peptone, 5g yeast extract, 5g sodium chloride, 1L distilled water, pH7.2.

[0055] In step (2), preparation of fermentation agent: the above-mentioned Bacillus subtilis DZSY21 seed liquid was inoculated in the fermentation liquid medium, and 350 mL of culture liquid was placed in a 1L conical flask, and the inoculation amount of the seed liquid was 2.0 %, the stirring rate is 160r / min, cultivated at 36°C for 9 hours, and adjust...

Embodiment 3

[0057] The difference between embodiment 3 and embodiment 1 is:

[0058] The preparation method of bacillus subtilis bacterial agent of the present invention, comprises the steps:

[0059] In step (1), the preparation of Bacillus subtilis seed liquid: inoculate Bacillus subtilis DZSY21 in the culture solution, place 50 mL of the culture solution in a 250 mL conical flask, stir at 160 r / min, and cultivate at 37 ° C for 16 hours, until the OD600 of the culture solution was 1.0, and the seed solution was obtained; the formula of the culture solution was: 10 g of peptone, 5 g of yeast extract, 5 g of sodium chloride, 1 L of distilled water, and pH 7.2.

[0060] In step (2), preparation of fermented bacterial agent: the above-mentioned Bacillus subtilis DZSY21 seed liquid is inoculated in the fermentation broth, 350 mL of culture liquid is placed in a 1L conical flask, and the inoculation amount of the seed liquid is 2.5 %, the stirring rate is 160r / min, cultivated at 37°C for 10 ...

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Abstract

The invention discloses bacillus subtilis and application thereof to prevention and treatment of bipolaris maydis. The strain of the bacillus subtilis for preventing and treating bipolaris maydis is DZSY21, the preservation name is bacillus subtilis, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) which is located in #3, No.1 Courtyard, West Beichen Road, Chaoyang District, Beijing, the preservation date is November 27, 2015, and the preservation No. is CGMCC NO.11749. The 16S rDNA gene sequence of the bacillus subtilis is a nucleotide sequence as shown in SEQ ID No.1, and the gyrB gene sequence of the bacillus subtilis is a nucleotide sequence as shown in SEQ ID No.2. The bacillus subtilis is high in growth speed, large in sporulation quantity, good in stress resistance and good in antibacterial property, and can be quantitatively colonized in leaves of corn plants.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to bacillus subtilis and its application in the prevention and treatment of maize spot disease. Background technique [0002] Southern corn leaf blight (Southern corn leaf blight) is one of the most important diseases that commonly occur in corn producing areas in the world. At present, the prevention and control of small spot disease mainly adopts methods such as planting resistant varieties and chemical control. Among them, chemical control is one of the key technologies to control corn diseases. However, excessive use of chemical fungicides is likely to cause problems such as ecological environment pollution and food safety. Therefore, biological control has gradually developed into an important green control technology for corn, especially fresh corn. In recent years, the important physiological and ecological functions of plant endophytes and their huge application pot...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/125
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/125
Inventor 丁婷苏博顾双月江海洋
Owner ANHUI AGRICULTURAL UNIVERSITY
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