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Preparation method of biological deodorant

A technology for biological deodorants and strains, applied in the directions of separation methods, separation of microorganisms, chemical instruments and methods, etc., can solve the problems of weak environmental adaptability of biological deodorants, complicated preparation methods of biological deodorants, and poor deodorization effect. Ideal and other problems, to achieve the effect of low production cost, long-lasting action time, and avoiding adsorption saturation

Pending Publication Date: 2021-03-19
苏州路易兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention provides a preparation method of a biological deodorant, which solves the problem that the preparation method of the current biological deodorant is complicated, and the prepared biological deodorant has weak adaptability to the environment, poor persistence of action, and poor deodorizing effect. suboptimal problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A preparation method of biological deodorant, comprising the following steps:

[0017] Step 1, separation and purification of strains; add the surface soil next to the chicken coop into a sterilized container filled with physiological saline, vibrate and disperse on an oscillator, and dilute with sterile water to a soil mass concentration of 10-2 The solution was coated on the separation medium by the plate coating method, and then placed in an incubator at 37°C for 12 hours at a constant temperature; the colonies with different characteristics on the plate were streaked and separated until pure culture was obtained, and set aside;

[0018] Step 2, screening of strains; inoculate the isolated strains into corresponding liquid medium for expansion; prepare air-dried pig manure into 50% pig manure water, and insert 10% of the inoculum into the expansion For the cultured strains, the deodorizing effect is judged every 4 hours, and the strains are screened through the deodo...

Embodiment 2

[0022] A preparation method of biological deodorant, comprising the following steps:

[0023] Step 1, separation and purification of strains; add the surface soil next to the piggery into a sterilized container filled with physiological saline, shake and disperse on an oscillator, and dilute with sterile water to a soil mass concentration of 10-2 The solution was coated on the separation medium by the plate coating method, and then placed in an incubator at 37°C for 12 hours at a constant temperature; the colonies with different characteristics on the plate were streaked and separated until pure culture was obtained, and set aside;

[0024] Step 2, screening of strains; inoculate the isolated strains into corresponding liquid medium for expansion; prepare air-dried pig manure into 50% pig manure water, and insert 10% of the inoculum into the expansion For the cultured strains, the deodorizing effect is judged every 4 hours, and the strains are screened through the deodorizing ...

Embodiment 3

[0028] A preparation method of biological deodorant, comprising the following steps:

[0029] Step 1, separation and purification of strains; add the surface soil next to the cowshed into a sterilized container filled with physiological saline, vibrate and disperse on a shaker, and dilute with sterile water to a soil mass concentration of 10-2 The solution was coated on the separation medium by the plate coating method, and then placed in an incubator at 37°C for 12 hours at a constant temperature; the colonies with different characteristics on the plate were streaked and separated until pure culture was obtained, and set aside;

[0030] Step 2, screening of strains; inoculate the isolated strains into corresponding liquid medium for expansion; prepare air-dried pig manure into 50% pig manure water, and insert 10% of the inoculum into the expansion After cultivating the strains, the deodorizing effect is judged every 4 hours, and the strains are screened through the 24-hour de...

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PUM

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Abstract

The invention discloses a preparation method of a biological deodorant, and belongs to the field of biology. The preparation method disclosed by the invention of the biological deodorant comprises thesteps of strain separation and purification, strain screening, strain compounding, loading preparation and the like. The invention solves the problems of complex preparation method of the existing biological deodorant, weak adaptive capacity of the prepared biological deodorant to the environment, poor action durability and unsatisfactory deodorization effect, and has the characteristics of highdeodorization efficiency and lasting deodorization performance.

Description

technical field [0001] The invention relates to the technical field of biological deodorization, in particular to a preparation method of a biological deodorant. Background technique [0002] With the development of large-scale animal husbandry, the problem of environmental pollution in livestock and poultry production is becoming more and more serious. The stench produced not only seriously affects the health and production performance of livestock and poultry in the farm, but also pollutes the environment around the farm. The main reason for the generation of malodorous gas from livestock and poultry manure is the volatilization and release of NH3, H2S and other gases during the accumulation process. Biological deodorants are environmentally friendly and can remove odors based on the basis. Therefore, they are gradually used in deodorization of animal husbandry. The basic principle of biological deodorant is to use microorganisms to absorb the malodorous substances in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D53/85B01D53/02B01D53/58B01D53/52B01D53/72C12N1/02C12N11/14
CPCB01D53/85B01D53/02B01D53/58B01D53/52B01D53/72C12N1/02C12N11/14B01D2258/0266B01D2257/702B01D2257/90Y02E50/30Y02A50/20
Inventor 张雅萍卢晨阳
Owner 苏州路易兴生物科技有限公司
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