Plant bacteriostatic composition and application thereof in cosmetics
A plant antibacterial and composition technology, applied in cosmetics, cosmetic preparations, applications, etc., to achieve good antibacterial effect and reduce the effect of adding amount
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Embodiment 1
[0024] Embodiment 1, preparation of licorice extract, honeysuckle extract and magnolia bark extract
[0025] In this example, the test raw materials licorice, honeysuckle, and magnolia bark were purchased from the common people's pharmacy. Prepare licorice extract, honeysuckle extract and magnolia bark extract respectively according to the following methods:
[0026] The licorice extract is extracted according to the following method: 10 g of dried Chinese medicinal materials (roots and rhizomes) of licorice are crushed through a 100-mesh sieve, and 80% ethanol is used as the extraction solvent according to the ratio of 20 mL of extraction solvent per gram of licorice , extract at 80°C for 2 hours, centrifuge and filter, collect the filtrate, and record it as the first filtrate; the filter residue is uniformly dispersed with ethanol with a concentration of 80% according to the ratio of 15mL extraction solvent per gram of filter residue, extract at 75°C for 3h, centrifuge, Fil...
Embodiment 2
[0029] Embodiment 2 antibacterial experiment
[0030] 1 material
[0031] 1.1 Test material
[0032] The licorice extract, honeysuckle extract and magnolia bark extract prepared in Example 1.
[0033] 1.2 Tested strains
[0034] Bacteria: Staphylococcus aureus: (Staphylococcus aureus) ATCC 6538
[0035] Escherichia coli: (Escherichia coli) ATCC 8739
[0036] Pseudomonas aeruginosa: (Pseudomonas.aeruginosa) ATCC 9027
[0037] Mold and yeast: Aspergillus niger ATCC 16404
[0038] Candida albicans ATCC 10231
[0039] Note: The above strains are provided by the Microbial Strain Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.
[0040] 1.3 Test medium
[0041] Mold culture medium: Cha's culture medium, sterilized under high pressure at 115°C for 30 minutes, and made into a slant for later use.
[0042] Bacterial medium: lecithin Tween 80-nutrient agar medium, sterilized under high pressure at 121°C for 20 minutes, and made into a slope for ...
Embodiment 3
[0066] Embodiment 3, the preparation of plant antibacterial composition
[0067] Take 300ml of licorice extract stock solution, 300ml of honeysuckle extract stock solution and 200ml of magnolia bark extract stock solution prepared according to the method of Example 1, mix and concentrate to 300ml to obtain the plant antibacterial composition of this embodiment. The plant antibacterial composition is tested, wherein the total flavonoid content is greater than 150mg / 100ml; the chlorogenic acid content is greater than 60mg / 100ml; the magnolol content is greater than 25mg / 100ml; the honokiol content is greater than 18mg / 100ml.
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