Integral nitrifying bacteria culture and preservation method as well as reactor

A nitrifying bacteria and reactor technology, applied in the field of storage devices for nitrifying bacteria cultivation, can solve problems such as low work efficiency, heavy workload, and increased probability of contamination by bacteria, and achieve cost saving, production process reduction, and domestication time shortening Effect

Inactive Publication Date: 2008-06-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of process has a large workload and low work efficiency, and also increases the probability of contamination by bacteria

Method used

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  • Integral nitrifying bacteria culture and preservation method as well as reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The operation process of the storage method of Nitrobacter victoria at room temperature and low dissolved oxygen is as follows:

[0028] (1) Preparation of culture preservation reactor. The reactor should be a sealable container, supplied by an air pump, the gas is pure oxygen, and the ventilation rate can be adjusted. The liquid filling capacity in the reactor is 75%.

[0029] (2) Medium solution preparation: 1000mL water containing NaHCO 3 1.0g, NaNO 2 2.2g, Na 2 CO 3 0.1g, NaCl0.8g, KH 2 PO 4 0.1g, MgSO 4 0.8g and FeSO 4 0.005g, pH 7.3.

[0030] (3) Nitrobacter victoria culture stage. The air is ventilated by the air pump, and the dissolved oxygen in the medium is ≥5mg / l through the bottom sparger, and the amount of nitrifying bacteria is monitored at the same time.

[0031] (4) Preparation for the preservation stage. When the concentration of Nitrobacter victoria reaches 1.87gMLSS / l, adjust the sparger so that it is 2 / 3 away from the top, and at the same...

Embodiment 2

[0035] The operation process of the storage method of Nitrobacter victoria at room temperature and low dissolved oxygen is as follows:

[0036] (1) Preparation of culture preservation reactor. The reactor should be a sealable container, supplied by an air pump, the gas is filtered air, and the ventilation rate can be adjusted. The liquid filling capacity in the reactor is 70%.

[0037] (2) Medium solution preparation: 1000mL water containing NaHCO 3 2.0g, NaNO 2 1.2g, Na 2 CO 3 1.0g, NaCl0.1g, KH 2 PO 4 0.8g, MgSO 4 0.1g and FeSO 4 0.1 g, pH 7.0.

[0038] (3) Nitrobacter victoria culture stage. The air is ventilated by the air pump, and the dissolved oxygen in the medium is ≥5mg / l through the bottom sparger, and the amount of nitrifying bacteria is monitored at the same time.

[0039] (4) Preparation for the preservation stage. When the amount of nitrifying bacteria reaches 1.83gMLSS / l, adjust the sparger so that it is 2 / 3 away from the top, and at the same time re...

Embodiment 3

[0043] The operation process of the storage method of Nitrobacter victoria at room temperature and low dissolved oxygen is as follows:

[0044](1) Preparation of culture preservation reactor. The reactor should be a sealable container, supplied by an air pump, the gas is filtered air, and the ventilation rate can be adjusted. The liquid filling capacity in the reactor is 70%.

[0045] (2) Culture medium solution preparation: 1000mL water contains NaHCO31.5g, NaNO 2 2.0g, Na 2 CO 3 0.6g, NaCl0.5g, KH 2 PO 4 0.5g, MgSO 4 0.4g and FeSO 4 0.008g, pH 7.5.

[0046] (3) Nitrobacter victoria culture stage. The air is ventilated by the air pump, and the dissolved oxygen in the medium is ≥5mg / l through the bottom sparger, and the amount of nitrifying bacteria is monitored at the same time.

[0047] (4) Preparation for the preservation stage. When the amount of nitrifying bacteria reaches 1.79gMLSS / l, adjust the sparger so that it is 2 / 3 away from the top, and at the same time...

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Abstract

The present invention discloses a method of culturing and storing integrated nitrobacteria and a reaction vessel. The distribution device of the reaction vessel is placed inside a tank body. An air inlet tube and an air outlet tube are respectively extended inside the tank body to be connected with the distribution device. The position of the distribution device inside the tank body can be adjusted up and down by the air inlet tube and the air outlet tube. The culturing step and the storing step are processed inside the same reaction vessel with the air inlet tube, the air outlet tube and an air distribution device. The culturing step is that the air distribution device is placed at the bottom of a vessel; the temperature is kept between 20 DEG C and 35 DEG C; culture medium dissolved oxygen is higher than 2.0mg/L. The storing step is that the temperature is kept between 20 DEG C and 35 DEG C; the air distribution device is adjusted to be higher than one third distance from the bottom; the culture medium dissolved oxygen is less than 0.8mg/L; the glycerin or glucose of 1.8 to 3.0g/L is added according to the cubage of the culture medium. The present invention ensures that the half-life of the nitrobacteria reaches 170 days to 220 days, reduces the production steps, improves the working efficiency and provides technical support for the large scale production of the nitrobacteria.

Description

technical field [0001] The invention relates to a storage device and method for cultivating nitrifying bacteria, in particular to a storage method and a storage reactor for nitrifying bacteria at normal temperature and low dissolved oxygen. Background technique [0002] Nitrifying bacteria (Nitrite Oxidizing Bacteria) are an important class of microorganisms that degrade nitrite into nitrate in the natural nitrogen cycle. Due to the severe damage to the micro-ecological environment of water bodies in recent years, the excessive accumulation of nitrite in large areas and poisoning incidents broke out frequently, which directly or indirectly caused huge economic losses in our country. However, the nitrifying bacteria products on the market in my country are extremely scarce, and most of them are substitutes for other types of low-efficiency mixed microorganisms, such as photosynthetic bacteria and bacillus. One of the main reasons for this situation is that the activity of ni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/20C12R1/01
Inventor 林炜铁任杰
Owner SOUTH CHINA UNIV OF TECH
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