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Method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using aerobic dynamic drainage method

A technology of polyhydroxyalkanoate and activated sludge, which is applied in the direction of fermentation, etc., can solve the problems of unstable operation of the reactor, high cost of the production method, and low PHA content, and achieve the promotion of industrial application, multiple cycles, and high synthesis speed fast effect

Active Publication Date: 2014-03-26
HARBIN INST OF TECH
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Problems solved by technology

[0004] The purpose of the present invention is to solve the high cost of the existing PHA production method, and the use of activated sludge mixed flora to synthesize PHA has the problems of long acclimation time, unstable operation of the reactor and low content of synthetic PHA, and provides The aerobic dynamic drainage method is used to quickly domesticate the mixed flora of activated sludge to prepare polyhydroxyalkanoate, which can not only save time and cost, but also increase the content of PHA in the mixed flora

Method used

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  • Method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using aerobic dynamic drainage method
  • Method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using aerobic dynamic drainage method

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specific Embodiment approach 1

[0019] Embodiment 1: The aerobic dynamic drainage method of this embodiment is used to quickly domesticate the mixed flora of activated sludge to prepare polyhydroxyalkanoate. The specific steps are as follows:

[0020] 1. Put the activated sludge in the SBR reactor with a height-to-diameter ratio of 10:1, so that the sludge concentration in the reactor reaches 3000-5000mg / L;

[0021] Two, take 6~12h as an operation cycle, run the SBR reactor, the operation process of a cycle of the SBR reactor is as follows: firstly carry out the first water inflow, after the first water inflow is completed, in the SBR reactor When the temperature is 20 ℃ ~ 25 ℃, start aeration, after the dissolved oxygen in the SBR reactor jumps from 2 ~ 3mg / L to 6 ~ 7mg / L, stop the aeration, and then carry out precipitation for 3 ~ 5min. Drainage; carry out the second water intake, continue to aerate for 5-11 hours, and drain after 30 minutes of sedimentation; among them, the first water intake contains car...

specific Embodiment approach 2

[0032] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the aeration is started when the temperature of the reactor is 23° C. as described in step 2. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0033] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the carbon sources are all small molecule organic acids. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using an aerobic dynamic drainage method and relates to a method for preparing the PHA. The invention solves the problems that the acclimatization time for synthesizing PHA through active sludge mixed flora is long, a reactor works unstably and the synthesized PHA is low in content in the prior art. The method comprises the following steps: 1, active sludge is put into a sequencing batch reactor (SBR); 2, 6-12 hours are taken as a cycle of operation, in the cycle of operation, two-time water introduction, precipitation and drainage are carried out, the drainage rate is 1:1, during the first water introduction, a carbon source and various nutrients are introduced, and during the second water introduction, only the supplement of nitrogen nutrient is carried out; 3, 2-4 cycles are operated every day, the sludge detention time is 1-10 days and sludge-water mixed liquid is collected; 4, batch experiments are carried out, half supernatant is discharged, equivalent carbon source is supplemented, aeration, precipitation and drainage are carried out, and the process is repeated for 6 to 8 times. The invention belongs to the field of biodegradable plastic synthesis.

Description

technical field [0001] The invention belongs to the field of utilization of municipal sludge resources and synthesis of biodegradable plastics, and in particular relates to the domestication of activated sludge mixed flora with the ability to synthesize polyhydroxyalkanoate (PHAs) by adopting an SBR aerobic process. Background technique [0002] Polyhydroxyalkanoates (PHAs or PHAs for short) are a class of lipid polymers synthesized by a variety of prokaryotic microorganisms under unbalanced nutritional conditions and stored in cells. They accumulate carbon sources in microbial metabolism. Balance the role of intracellular and extracellular ions. Its chemical and physical properties are similar to chemically synthesized plastics, and PHA can be completely decomposed and utilized by microorganisms in nature. It is a new type of environmentally friendly material with great application prospects and development value. It is expected to solve the current widespread white polluti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62
Inventor 温沁雪郭子瑞陈志强黄龙
Owner HARBIN INST OF TECH
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