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Biological product containing aerobe combined bacteria and preparation method and application thereof

A technology for aerobic microorganisms and biological products, applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems affecting the promotion of dominant bacteria products, high cost, difficulty in maintaining the depth of dominant bacteria, etc. The effect of acclimation and debugging time, preventing loss and improving effluent quality

Inactive Publication Date: 2015-03-18
房来喜 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this adding method is difficult to maintain the depth of dominant bacteria in the system, because some microorganisms will be discharged from the system together with the remaining sludge during use
Therefore, in order to maintain the concentration of dominant bacteria, it must be replenished regularly, and the required cost is very high, which affects the promotion of dominant bacteria products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The porous carrier used in this embodiment is polyurethane foam, its average pore diameter is about 0.5 ~ 1mm, and its average volume is 20cm 3 . Soak in HCl solution with a concentration of 1% (percentage by weight) for 3 hours before use to remove residual isocyanate. Then, wash with water until neutral, squeeze dry and set aside.

[0039] The prepared bacteria concentration is l0 8 10% (volume percentage) of urban sewage was added to the aerobic microbial combination liquid (the content of Bacillus subtilis and Pseudomonas putida at a ratio of 1:2) per ml, cultivated and acclimatized at room temperature for 5 days. Then take out 200mL bacterial liquid, add 3wt% sodium alginate, dissolve evenly, to increase the viscosity of mixed bacterial liquid, then, add the carrier that volume is 2L through above-mentioned cleaning, make bacterial liquid be attached on carrier as evenly as possible (dipping method load). The average loading capacity of the total bacterial cell...

Embodiment 2

[0044] The used porous carrier of this embodiment is polyurethane foam plastics, and aperture is about 0.5mm, and each volume is about 25cm 3 . Soak in HCl solution with a concentration of 1% (weight percent concentration) for 2 hours before use to remove residual sulfur compounds and the like. Then, wash with water until neutral, and let it dry for later use.

[0045] The prepared concentration of each bacteria is 10 8 Add 5g of sodium alginate to 200ml of aerobic microbial combination bacteria liquid per ml (the content of Bacillus subtilis and Pseudomonas putida is 1:2). The liquid is loaded on the carrier as uniformly as possible. The average loading capacity of the total bacterial cells of the bacterial liquid on each carrier is about 10 7 piece / cm 3 , so as to obtain biological products containing aerobic microbial combination bacteria.

[0046] The above-mentioned products were installed in the small test device of Example 1, and tested after running for one week....

Embodiment 3

[0053] The porous carrier used in this experiment example is cinder, and the particle volume is about 4-10 cm 3 wait. The hole diameter ranges from 0.1 to 4mm. Soak in 1vol% NaOH solution for 12 hours before use, then rinse with water to neutral and dry. Then, according to the method described in Example 2, a biological product containing aerobic microbial combination bacteria was prepared, and the sewage treatment test was carried out using the same process and conditions as in Example 1. The results show that the effects obtained by using these two carriers to treat sewage are similar.

[0054] Water volume 180L / day, hydraulic retention time HRT=8h.

[0055] Influent water: COD=150~500mg / L, average 250mg / L; ammonia nitrogen content=30~50mg / L; average 40mg / L

[0056] Effluent: COD=30~55mg / L, average 45mg / L; ammonia nitrogen content<1.5mg / L.

[0057] No sludge was detected in the secondary settling tank.

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PUM

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Abstract

The invention discloses a biological product containing aerobe combined bacteria and a preparation method and application thereof. The biological product includes aerobe combined bacteria loaded on a porous carrier; and the aerobe combined bacteria includes bacillus and pseudomonas putida. The preparation method includes the steps of: 1) soaking the porous carrier with acid or alkali and rinsing the porous carrier with water to a neutral state to obtain pretreated porous carrier; and 2) loading the aerobe combined bacteria liquid on the porous carrier. The biological product can be used in sewage treatment, and significantly improve the efficiency of biochemical treatment, efficiently remove COD and ammonia nitrogen, improve the outlet water quality and reduced the sludge yield by more than 80%.

Description

technical field [0001] The invention relates to a biological product and its preparation method and application, in particular to a biological product containing aerobic microbial combination bacteria and its preparation method and application. Background technique [0002] Environmental microorganisms are ubiquitous in nature, have the characteristics of wide source, fast reproduction speed, and strong adaptability to the environment, and have been widely used in the field of water treatment. Many aerobic microorganisms oxidize and decompose organic matter into CO through catabolism, anabolism, and material mineralization in the presence of oxygen. 2 and H 2 O, to purify the water quality, and in the process, obtain C, N, P, S and energy to enable itself to reproduce. Among them, the activated sludge method has been developed for nearly a hundred years since it was discovered and applied by British scientists Clark and Cage in 1912. It has been perfected in technology and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08C12N11/04C12N1/20C02F3/34C12R1/40C12R1/125C02F103/20
Inventor 房来喜平郑骅朱炜袁唯
Owner 房来喜
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