Subcutaneous fluorescent labeling method for Sepiella maindroni and special reagent thereof

Abstract

The invention discloses a subcutaneous fluorescent labeling method for Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. A special labeling reagent for the subcutaneous fluorescent labeling method for the Sepiella maindroni contains one portion of alizarin complexometric indicator powder and four to eight portions of yoghurt according to weight portion and forms a label in a subcutaneous area which is relatively concentrated, and the label has higher identifiability than a general staining label. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

Description

technical field [0001] The invention relates to a labeling method for aquatic animals and a special reagent thereof, in particular to a subcutaneous fluorescent labeling method and a special reagent for the squid without needles. Background technique [0002] Mansoni squid was once one of the four major fisheries in my country's fishing grounds. It has strong adaptability to temperature and is widely distributed in my country's coastal areas. It is a traditional fishery species with high economic value. Due to overfishing and ecological deterioration, since the 1970s, there have been successive declines. In the 1980s, it was reported that Li Xingjie et al. conducted preliminary research on the resources and multiplication of squid squid, while there were reports on the biology and breeding of squid abroad. So far, relevant scientific research institutions have initially solved the reproductive regulation, artificial breeding, seedling cultivation and cultivation techniques ...

AI Technical Summary

Problems solved by technology

However, the marking effect is not obvious, and the floating color of the marking will remain on the body surface for a short period of time. Release the squid marked by this method into the natural sea area will affect its own protective color, which is not conducive to its survival in the natural environment. Dyeing and marking the tail end of the inner shell of the golden squid will no longer be applicable to individuals of the genus Squid