Artificial insemination incubation method for sepiella maindroni and special agent used thereof

A technique of Mansfield needleless squid and reagents, which is applied in the field of Mansfield needleless squid artificial insemination incubation method and special reagents, and can solve problems such as no precedent.

Inactive Publication Date: 2009-05-13
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
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  • Artificial insemination incubation method for sepiella maindroni and special agent used thereof

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Embodiment

[0020] Embodiment: a special reagent for artificial insemination of Mansoni squid artificial insemination, which contains a culture container bottom liner and a nutrient solution; the culture container bottom liner is 0.2% agarose; the nutrient solution contains 1 part of serum or 1 part by weight. oviductal gland secretion liquid or its combination; the nutrient solution also contains 500 parts by weight of sterile sea water. In the culture stage 2 days before fertilization, 1 part of the oviductal gland secretion of Squid mansoni was used alone, and after 2 days, 1 part of serum plus 1 part of oviductal gland secretion was used as the nutrient-providing culture solution. Experiments have shown that in this way the embryo can normally complete the entire development process.

[0021] Sterile seawater is well-aerated seawater filtered through micropores, that is, artificially aerated so that its oxygen content is at least not lower than that of ordinary natural seawater. Ster...

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Abstract

The invention discloses a cuttle-fish artificial insemination and incubation method and special agent thereof, wherein the eggs in oviduct of the female cuttle-fish are transferred into a container with nutrient solution to perform external insemination and isolated culture and an agarose layer is set between the container bottom and eggs; the nutrient solution contains the fresh or freeze-dry oviduct gland secretion of the female cuttle-fish. The special agent contains culture container bottom liner and nutrient solution and the culture container bottom liner is 0.2% of agarose and the nutrient solution contains 1-10 portions by weight of blood serum and 1000 portions by weight of sterile sea water. Because the molecular structure of agarose is loose, the nutrient material can freely flow and penetrate, the eggs on the agarose liner has little distortion without nutrition deficiency of the contact surface, The nutrition is provided by the oviduct gland secretion and the blood serum of low concentration, the eggs do not distort, thus the whole development process of the cuttle-fish embryo cultured in vitro is greatly accomplished.

Description

technical field [0001] The invention relates to an artificial insemination incubation method for aquatic animals and a special incubation reagent thereof, in particular to an artificial insemination incubation method for squid mansoni and its special reagent. Background technique [0002] Mansoni squid was once one of the four major fisheries in my country's fishing grounds. It has strong adaptability to temperature and is widely distributed in my country's coastal areas. It is a traditional fishery species with high economic value. Due to overfishing and ecological deterioration, since the 1970s, there have been successive declines. In the 1980s, it was reported that Li Xingjie et al. conducted preliminary research on the resources and multiplication of squid squid, while there were reports on the biology and breeding of squid abroad. In order to carry out artificial propagation and breeding of squid, the basic biological research of squid must be carried out first. The r...

Claims

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Application Information

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IPC IPC(8): A01K61/00A61D19/02
CPCY02A40/81
Inventor 徐梅英桂福坤徐佳晶吴常文
Owner ZHEJIANG OCEAN UNIV
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