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Subcutaneous alizarin red S marking method for sepiella maindroni and special marking reagent thereof

A Mansfield needle-free squid and marking method technology, applied in chemical instruments and methods, application, climate change adaptation and other directions, can solve the problems of insignificant marking effect, floating color residue, unfavorable survival, etc., and achieve high recognizability , easy to operate, reduce damage effect

Inactive Publication Date: 2010-06-23
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the marking effect is not obvious, and the floating color of the marking will remain on the body surface for a short period of time. Release the squid marked by this method into the natural sea area will affect its own protective color, which is not conducive to its survival in the natural environment. Dyeing and marking the tail end of the inner shell of the golden squid will no longer be applicable to individuals of the genus Squid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: First, sterilize a 6ml syringe in 90-95% alcohol for 10-15 minutes. Then remove and pipette 3.0ml of pasty ARS marker. Put a cuttlefish individual with a carcass length of 1.1cm into the MS-SSS anesthetic solution with a concentration of 60ppm for about 0.5min, then take it out immediately and put it on the operating table covered with a soaked towel. The syringe needle is pierced through the outer skin obliquely at an angle of about 12° from the horizontal plane from the lower end of the back of the squid individual to the head, and the needle enters the outer skin by about 0.3 cm, so that it is between the middle membrane and the outer skin. In the process of pulling out the needle, push the syringe with proper force to inject the pasty ARS marker in the syringe into strips under the outer skin of the squid. Immediately after needle withdrawal, the marked individual was resuscitated in clean seawater.

Embodiment 2

[0012] Example 2: First, sterilize a 6ml syringe in 90-95% alcohol for 10-15 minutes. Then remove and pipette 3.0ml of pasty ARS marker. Put a cuttlefish individual with a carcass length of 3.5cm into the MS-SSS anesthetic solution with a concentration of 60ppm for about 2.0min, then take it out immediately and put it on the operating table covered with a soaked towel. The needle of the syringe is pierced through the outer skin at an angle of about 12° to the horizontal plane from the front end of the back of the squid individual to the tail, and the needle enters the outer skin about 0.8 cm, so that it is between the middle membrane and the outer skin. In the process of pulling out the needle, push the syringe with proper force to inject the pasty ARS marker in the syringe into strips under the outer skin of the squid. Immediately after needle withdrawal, the marked individual was resuscitated in clean seawater.

Embodiment 3

[0013] Embodiment 3: First, sterilize the 10ml syringe in 90-95% alcohol for 10-15min. Then remove and pipette 3.0ml of pasty ARS marker. Put a cuttlefish individual with a carcass length of 6.2cm into the MS-SSS anesthetic solution with a concentration of 60ppm for about 3.5min, then take it out immediately and put it on the operating table covered with a soaked towel. The needle of the syringe is pierced through the outer skin at an angle of about 12° to the horizontal plane from the front end of the back of the squid individual to the tail, and the needle enters the outer skin about 1.2 cm, so that it is between the middle membrane and the outer skin. In the process of pulling out the needle, push the syringe with proper force to inject the pasty ARS marker in the syringe into strips under the outer skin of the squid. Immediately after needle withdrawal, the marked individual was resuscitated in clean seawater.

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PUM

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Abstract

The invention discloses a subcutaneous alizarin red S marking method for sepiella maindroni. The method comprises the following steps: firstly, anesthesia step: selecting sepiella maindroni to be marked into MS-222 anesthetic solution with a concentration of 40ppm to 70ppm for 0.5 to 4 minutes; secondly, marking step: injecting dyeing liquor into the inner shell of the sepiella maindroni treated in step one with an injector. Because the MS-222 anesthetic solution is used for performing anesthesia processing to the sepiella maindroni, the sepiella maindroni basically has no stress when being injected with dyeing liquor, thereby being convenient for operation and reducing the hurt to the sepiella maindroni. The special marking reagent for the subcutaneous alizarin red S marking method for sepiella maindroni comprises 1 part of alizarin red S powder and 4-8 parts of yoghourt according to part by weight, marks can be formed in relatively concentrated subcutaneous areas and are more highly identifiable than the ordinary dyeing marks. In addition, by using the method, after being resuscitated, the sepiella maindroni is not required to be raised temporarily to remove the floating color but can be freed immediately.

Description

technical field [0001] The invention relates to a method for marking aquatic animals and a special labeling reagent thereof, in particular to a method for labeling subcutaneous Alizarin Red S of squid without needles and a special labeling reagent thereof. Background technique [0002] Mansoni squid was once one of the four major fisheries in my country's fishing grounds. It has strong adaptability to temperature and is widely distributed in my country's coastal areas. It is a traditional fishery species with high economic value. Due to overfishing and ecological deterioration, since the 1970s, there have been successive declines. In the 1980s, it was reported that Li Xingjie et al. conducted preliminary research on the resources and multiplication of squid squid, while there were reports on the biology and breeding of squid abroad. So far, relevant scientific research institutions have initially solved the reproductive regulation, artificial breeding, seedling cultivation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C09B67/42C09B1/02C09K11/06
CPCY02A40/81
Inventor 徐梅英张建设迟长凤吴常文
Owner ZHEJIANG OCEAN UNIV
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