Fluorescent labeling method for inner shell of Sepiella maindroni

A Man's needle-free squid, fluorescent labeling technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of floating color residue, unfavorable survival, and inconspicuous labeling effect, so as to achieve easy operation and reduce damage. Effect

Inactive Publication Date: 2010-06-16
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the marking effect is not obvious, and the floating color of the marking will remain on the body surface for a short period of time. Release the squid marked by this method into the natural sea area will affect its

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: First, sterilize a 1ml syringe in 90% to 95% alcohol for 10 minutes to 15 minutes. Then take out and absorb 51ppm of alizarin complex indicator (ALC, C 19 h 15 NO 8 ) solution 0.5ml. Put an individual cuttlefish with a carcass length of 1.1cm into the MS-222 anesthetic solution with a concentration of 60ppm for about 0.5min, then take it out immediately and put it on the operating table covered with a water-soaked towel. Pierce the epidermis with the needle of the syringe from the lower end of the back of the squid individual to the head at an angle of about 15° to the horizontal plane. The needle enters the epidermis for about 0.3 cm. When you feel the sound of friction between the needle tip and the inner shell, it means that the needle has penetrated. The inner epidermis of the back of the squid individual is in contact with the inner shell. Gently push the syringe to inject the ALC solution under the epidermis. As the ALC is injected and diffuses o...

Embodiment 2

[0011] Embodiment 2: First, sterilize a 1ml syringe in 90% to 95% alcohol for 10-15 minutes. Then take out and suck 0.5ml of 380ppm ALC solution. Put a cuttlefish individual with a carcass length of 3.5cm into the MS-222 anesthetic solution with a concentration of 60ppm for about 2.0min, then take it out immediately and put it on the operating table covered with a soaked towel. Pierce the epidermis with the syringe needle from the front end of the back of the squid individual to the tail at an angle of about 15° to the horizontal plane. The needle enters the epidermis for about 0.8 cm. When you feel the sound of friction between the needle tip and the inner shell, it means that the needle has penetrated the squid. The inner epidermis of the individual's back is in contact with the inner shell. Gently push the syringe to inject the ALC solution under the epidermis. The needle was withdrawn, and the marked instance was immediately resuscitated in clean seawater.

Embodiment 3

[0012] Embodiment 3: First, sterilize a 1ml syringe in 90% to 95% alcohol for 10-15 minutes. Then take out and suck 0.5ml of 150ppm ALC solution. Put a cuttlefish individual with a carcass length of 6.2cm into the MS-222 anesthetic solution with a concentration of 60ppm for about 3.5min, then take it out immediately and put it on the operating table covered with a soaked towel. Pierce the epidermis with the syringe needle from the front end of the back of the squid individual to the tail at an angle of about 15° to the horizontal plane. The needle enters the epidermis for about 1.2 cm. When you feel the sound of friction between the needle tip and the inner shell, it means that the needle has penetrated the squid. The inner epidermis of the individual's back is in contact with the inner shell. Gently push the syringe to inject the ALC solution under the epidermis. The needle was withdrawn, and the marked instance was immediately resuscitated in clean seawater.

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PUM

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Abstract

The invention discloses a fluorescent labeling method for the inner shell of Sepiella maindroni, comprising the following steps of: (1) anesthesia: selecting Sepiella maindroni to be labeled and placing the Sepiella maindroni into an anesthetic solution MS-222 with the concentration ranging from 40ppm to 70ppm for 0.5 minute to 4 minutes; and (2) labeling: injecting a staining solution into the inner shell of the Sepiella maindroni treated in the step (1) with an injector. Because the invention performs anesthesia treatment on the Sepiella maindroni by using the anesthetic solution MS-222, the Sepiella maindroni basically has no stress when injected, the operation can be conveniently carried out, and the harm to the Sepiella maindroni can be reduced. In addition, a mode of staining solution injection is adopted, the injection is carried out at an acute angle, thus, the staining solution can be uniformly distributed on the inner shell favourably, and the invention is especially suitable for Sepiella. Moreover, the method does not need temporary culture for removing floating color after the Sepiella maindroni recovers, and the Sepiella maindroni can be released immediately.

Description

technical field [0001] The invention relates to a method for marking aquatic animals, in particular to a method for fluorescently marking the inner shell of squid mansoni. Background technique [0002] Mansoni squid was once one of the four major fisheries in my country's fishing grounds. It has strong adaptability to temperature and is widely distributed in my country's coastal areas. It is a traditional fishery species with high economic value. Due to overfishing and ecological deterioration, since the 1970s, there have been successive declines. In the 1980s, it was reported that Li Xingjie et al. conducted preliminary research on the resources and multiplication of squid squid, while there were reports on the biology and breeding of squid abroad. So far, relevant scientific research institutions have initially solved the reproductive regulation, artificial breeding, seedling cultivation and cultivation techniques of squid mansoni, providing an important foundation for th...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 吴常文董智勇郭宝英徐梅英
Owner ZHEJIANG OCEAN UNIV
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