Change feature analysis method of liver mtDNA copy number in sepiella maindroni aging process

Abstract

The invention provides a change feature analysis method of liver mtDNA copy number in the sepiella maindroni aging process. The method includes: sample genome DNA extraction, single copy gene primer design, real-time quantitative PCR standard preparation and standard curve establishing of nuclear genome and mitochondrial genome single copy gene, detection of mitochondrial genome copy number in a sample and content calculation, wherein a sample DNA extraction method includes: adding sepiella maindroni tissue into polybutylene succinate powder and alkyl ether ammonium sulfate, grinding together,and performing extraction, organic solvent separation and precipitation to obtain sample DNA. By applying fluorescent quantitative PCR technology, accurate detection and quantification of mitochondria can be realized, and analyzing change of liver mtDNA copy number in the sepiella maindroni aging process is conducive to providing a solution for increasing spawning rate and providing new referencefor studying human aging.

Description

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AI Technical Summary

Benefits of technology

This patented technique allows us to determine if there're any substances found inside or outside our body called mannitol (a sugar molecule). These substance help with various processes like cell division, growth control, blood clotting, muscle regenerative activity, etc., making them easier to separate from other materials than what they were beforehand.

Problems solved by technology

This patented technical problem addressed in this patents describes how old or damaged piglets may develop necrosis called amputation syndrome which causes dysfunction of various organs like kidneys and lungs. These symptoms include reduced fertility rates among young individuals, increased susceptibility towards environmental factors including pollution, lack of exercise, excess weight gain, decreased immune response, and potential health issues associated with malaria parasites. To address these challengings, the inventors developed an assays technique called Minute Spindler Manorex™ that uses modified nucleosome structures derived from mtDNAs to monitor the activity levels within the body's interior space. It achieves quick and precise analysis through qPCR techniques, allowing us to better predict future risk exposure based upon previous data collected about past incidents involving sea water sedimentation basins.

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